Volume 11, Issue 44 (9-2021)
NCMBJ 2021, 11(44): 29-40 |
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Department of Biology, Faculty of Science, Damghan Branch, Islamic Azad University, Damghan, Iran
Abstract: (2910 Views)
Aim and Background: Microorganisms are more important than other sources in dye production because microorganisms have advantages such as rapid growth, higher yields, and easier extraction. Natural pigments are healthier than chemical types and their use is useful in various industries. The aim of this study was to isolate and extract the pyocyanin pigment from Pseudomonas aeruginosa isolates in order to preparation of colored paper and candle.
Material and Methods: In this study, sampling was performed from different hospital environments in order to isolate P. aeruginosa. Environmental isolates of P. aeruginosa were identified using morphological features and differential biochemical methods. Confirmatory identification was performed by tracing the pbo1 gene using colony PCR. After extracting pyocyanin pigment with chloroform, finally, the produced pyocyanin was used to prepare colored paper and candle and the optical stability of pyocyanin was investigated.
Results: From 43 environmental samples collected, 15 isolates of P. aeruginosa were identified and confirmed by molecular method. The mean of optical absorption (OD520) of pyocyanin after extraction was 0.488. Staining of paper and candle with the extracted pyocyanin was successful and the optical stability of pyocyanin was favorable.
Material and Methods: In this study, sampling was performed from different hospital environments in order to isolate P. aeruginosa. Environmental isolates of P. aeruginosa were identified using morphological features and differential biochemical methods. Confirmatory identification was performed by tracing the pbo1 gene using colony PCR. After extracting pyocyanin pigment with chloroform, finally, the produced pyocyanin was used to prepare colored paper and candle and the optical stability of pyocyanin was investigated.
Results: From 43 environmental samples collected, 15 isolates of P. aeruginosa were identified and confirmed by molecular method. The mean of optical absorption (OD520) of pyocyanin after extraction was 0.488. Staining of paper and candle with the extracted pyocyanin was successful and the optical stability of pyocyanin was favorable.
Conclusion: Due to the rapid growth of bacteria and the possibility of its mass production in a short time and based on the results of this study, bacterial pigments can be used in various industries, including paper industry, and replaced by chemical dyes.
Type of Study: Research Article |
Subject:
Cellular and molecular
Received: 2021/02/22 | Accepted: 2021/09/14 | Published: 2021/09/14
Received: 2021/02/22 | Accepted: 2021/09/14 | Published: 2021/09/14
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