Volume 12, Issue 45 (11-2021)
NCMBJ 2021, 12(45): 87-96 |
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Aghaei P, Tabatabaee Bafroee A S, Shahhosseini M H. Polymerase Chain Reaction (PCR) technique for rapid detection of Cytomegalovirus in idiopathic abortions. NCMBJ 2021; 12 (45) :87-96
URL: http://ncmbjpiau.ir/article-1-1443-en.html
URL: http://ncmbjpiau.ir/article-1-1443-en.html
Department of Microbiology, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran
Abstract: (2512 Views)
Aim and Background Cytomegalovirus (CMV) is a member of the herpesviridae family, which is a major cause of intrauterine viral infection. The aim of this study was to evaluate the diagnosis of CMV in women with idiopathic abortions by the polymerase chain reaction (PCR) technique.
Materials and Methods 50 blood samples of women with idiopathic abortions were collected and viral genome was extracted using commercial kit DNG-Plus. The PCR technique was optimized using the standard CMV strain genome and then the Limit of Detection (LOD) and specificity of the test was evaluated using genomes other than CMV. The optimized PCR technique was performed on the samples and positive and negative controls and the results were analyzed by gel electrophoresis.
Results using optimized PCR technique, 257bp product were observed in agarose gel. In the specificity test, positive results were obtained only with the CMV genome and a detection limit of 100 copies per reaction was obtained. Finally, out of 50 samples, two samples were positive for CMV.
Conclusion Idiopathic abortions are abortions that do not seem to have a specific cause. But obviously many factors including infectious agents that can cause abortion are difficult to identify them. One of these factors could be CMV and the results of this study showed that it is best to search and identify CMV in this type of abortion by molecular tests such as PCR, quickly.
Materials and Methods 50 blood samples of women with idiopathic abortions were collected and viral genome was extracted using commercial kit DNG-Plus. The PCR technique was optimized using the standard CMV strain genome and then the Limit of Detection (LOD) and specificity of the test was evaluated using genomes other than CMV. The optimized PCR technique was performed on the samples and positive and negative controls and the results were analyzed by gel electrophoresis.
Results using optimized PCR technique, 257bp product were observed in agarose gel. In the specificity test, positive results were obtained only with the CMV genome and a detection limit of 100 copies per reaction was obtained. Finally, out of 50 samples, two samples were positive for CMV.
Conclusion Idiopathic abortions are abortions that do not seem to have a specific cause. But obviously many factors including infectious agents that can cause abortion are difficult to identify them. One of these factors could be CMV and the results of this study showed that it is best to search and identify CMV in this type of abortion by molecular tests such as PCR, quickly.
Type of Study: Research Article |
Subject:
Microbiology
Received: 2021/08/17 | Accepted: 2021/10/30 | Published: 2021/12/22
Received: 2021/08/17 | Accepted: 2021/10/30 | Published: 2021/12/22
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