Volume 12, Issue 46 (3-2022)                   NCMBJ 2022, 12(46): 89-99 | Back to browse issues page

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Tavakoli A, Kheiri H, Zarrinpour V, Forghanifard M. Evaluation of YKL39 target gene expression and its miR in the tissues of glioblastoma multiforme patients. NCMBJ. 2022; 12 (46) :89-99
URL: http://ncmbjpiau.ir/article-1-1452-en.html
1. Department of Biology, Faculty of Basic Sciences, Islamic Azad University Damghan Branch, Damghan, Iran
Abstract:   (750 Views)
Aim and Background: One of the most malignant and common brain tumors is glioblastoma multiforme (GBM). Patient survival is relatively poor, and due to the difficulty of early diagnosis, most patients die within one year of diagnosis. The aim of this study was to investigate the expression of YKL39 as a possible biomarker in glioblastoma multiforme and to evaluate the miR of this gene.

Materials and Methods: In this study, 25 tissue samples with glioblastoma multiforme before treatment and 25 samples of healthy tissue with glioblastoma multiforme tumor were collected as a control sample. RNA extraction and cDNA synthesis were performed. YKL39 gene expression was assessed using real-time PCR and ACTB gene was used as internal control. Statistical analysis of data was performed using GraphPad Prism software version 8. The Roc curve was used to evaluate the biomarker value of YKL39 gene.

Results: High expression of YKL39 (P **** <0.0001) was observed in glioblastoma tissue samples. Expression showed that YKL39 gene was not statistically significant with the age of patients with P value = 0.2971 and gender with P value = 0.888.

Conclusion: The increase in YKL39 gene expression in glioblastoma tissue samples is significant compared to healthy tumor margin tissue. The Roc curve showed that the YKL39 gene could not have biomarker value.
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Type of Study: Research Article | Subject: Genetics
Received: 2021/11/13 | Accepted: 2021/12/13 | Published: 2022/04/11

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