Aim and Background . The importance of effective parameters on b iosorption of Cu and Mn ions from Sarcheshme Copper Mine Acid Mine Drainage (AMD) was investigated using regression analysis.

 Materials and Methods. Bacillus Thuringiensis was isolated from Sarcheshme AMD water and sorption parameters of that AMD water were investigated.

Results. Correlation between amount of biosorbent and amount of Cu removed (0.99) was greater than the correlation between amount of biosorbent and Mn removed (0.98) from wastewater. Furthermore, increasing pH increased Cu and Mn ions removal up to 19 and 9 times, respectively. It was also observed that increasing contact time raised the Cu and Mn uptake 4.83 and 1.5 times, respectively.

Conclusion. Results showed that parameters like amount of biosorbent, pH, temperature and contact time have the most impact on amount of biosorption respectively and variation of effective parameters have more effects on Cu removal from wastewater.