Volume 5, Issue 17 (3-2015)
NCMBJ 2015, 5(17): 89-95 |
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Behrouznasab K, Razavi M R, Fathalian F, Seirafi H, Nejadsattari T. Diagnosis of skin infections caused by atypical Mycobacterium using PCR method. NCMBJ 2015; 5 (17) :89-95
URL: http://ncmbjpiau.ir/article-1-402-en.html
URL: http://ncmbjpiau.ir/article-1-402-en.html
Kasra Behrouznasab 
, Mohammad Reza Razavi , Fatollah Fathalian , Hassan Seirafi , Taher Nejadsattari
, Mohammad Reza Razavi , Fatollah Fathalian , Hassan Seirafi , Taher Nejadsattari
Science and Research Branch, Islamic Azad University , Kbehrouznasab@gmail.com
Abstract: (9052 Views)
Aim and Background: Granulomatous skin infections probably originated from an environment and through skin wounds, scratching, trauma, surgery into the inoculated skin, and abscess pus appears and sometimes is in form of Ulcerativa. Infection is limited to the skin and in case of immunosuppression would transmit and spread in form of infection. PCR is a rapid sensitive, specific and accurate method for diagnosis of bacterial infections and pathogens in the shortest time.
Materials and Methods: In this study atypical Mycobacterium granulomatous skin infections diagnosed by PCR, using specific primers design for Mycobacterium genes (16srRNA) and confirmation by sequencing methods (sequencing of nucleotides of) atypical mycobacterium and comparing with genome sequencing of 16srRAN genes atypical mycobacterium in Gene Bank and application of this technique in the production of atypical mycobacterium skin infections diagnostic kits.
Result: Among 58 studied paraffin blocks samples of skin tissue, 18 samples were in the range of positive control and established bands showing positive PCR results. Out of 18 positive samples of Mycobacterium granulomatous skin lesions, 83% were men and 17% women. In 13 cases (72%) there were occupational causes and in (28%) lack of occupational causes. 66% of mycobacterium granulomatous skin infections (NTM) were in adults over age 40 years.
Conclusion: PCR is a rapid, specific, sensitive and accurate method for the diagnosis of bacterial infections and pathogens in the shortest time. This method can recognize a few atypical Mycobacterium genomes in samples, which is the superior advantage of this method, compared to conventional pathological methods such as culture and differential biochemical experiments.
Materials and Methods: In this study atypical Mycobacterium granulomatous skin infections diagnosed by PCR, using specific primers design for Mycobacterium genes (16srRNA) and confirmation by sequencing methods (sequencing of nucleotides of) atypical mycobacterium and comparing with genome sequencing of 16srRAN genes atypical mycobacterium in Gene Bank and application of this technique in the production of atypical mycobacterium skin infections diagnostic kits.
Result: Among 58 studied paraffin blocks samples of skin tissue, 18 samples were in the range of positive control and established bands showing positive PCR results. Out of 18 positive samples of Mycobacterium granulomatous skin lesions, 83% were men and 17% women. In 13 cases (72%) there were occupational causes and in (28%) lack of occupational causes. 66% of mycobacterium granulomatous skin infections (NTM) were in adults over age 40 years.
Conclusion: PCR is a rapid, specific, sensitive and accurate method for the diagnosis of bacterial infections and pathogens in the shortest time. This method can recognize a few atypical Mycobacterium genomes in samples, which is the superior advantage of this method, compared to conventional pathological methods such as culture and differential biochemical experiments.
Keywords: Mycobacterial
Type of Study: Research Article |
Subject:
Microbiology
Received: 2013/10/11 | Accepted: 2014/02/23 | Published: 2015/03/1
Received: 2013/10/11 | Accepted: 2014/02/23 | Published: 2015/03/1
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