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Falsafi S, Zaker Bostanabad S, Feizabadi M M, Khavari-Nejad R A. Comparison and optimization of methods for isolation of Non-Tuberculous mycobacteria from surface water. NCMBJ 2014; 4 (15) :115-121
URL: http://ncmbjpiau.ir/article-1-562-en.html

Materials and Methods: 38 samples were collected from surface waters in Tehran. Water sampling was carried out in a volume of 200-100 ml. water sample was transferred immediately to the laboratory and examined. Three methods (Cetylpyridinium chlori, Petroff, Tacquet-Tison) for the isolation of mycobacteria were compared by applying them in parallel to 38 samples of surface water. Each method was defined by a particular combination of decontamination method. The efficacy of each method was determined by calculating the positivity, negativity, and contamination rates, the mean numbers of mycobacterial colonies grown and number of different mycobacterial strains isolated. The last value was determined by subjecting the isolates to PCR. 

Results: Decontamination with CPC appeared to be the best decontamination method, on the one hand, it significantly decreased the level of non-target microorganisms and, on the other hand, it was significantly less lethal for the NTM strains studied. 

Conclusion: Our goal was to measure the effects of various methods known to inhibit the growth of non-target microorganisms, while we also took into consideration the inhibitory effects of these methods on the growth of NTM. We propose that CPC procedure could be used for detection of NTM in aquatic samples.