Volume 5, Issue 20 (10-2015)                   NCMBJ 2015, 5(20): 47-54 | Back to browse issues page

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Asgari F, Falak R, Bahar M A, majd Z, Heydari nasrabadi M, Dashti S et al . The evaluation of oral probiotic feeding on T cell subsets infiltration in chicken as experimental model.. NCMBJ 2015; 5 (20) :47-54
URL: http://ncmbjpiau.ir/article-1-698-en.html
Faculty of Medicine, Iran University of medical science, Tehran, Iran , m_shekarabi@yahoo.com
Abstract:   (8634 Views)
Aims and background: This study was performed to evaluate the effect of lactobacillus acidophilus Bacteria (LAB) on chicken T cell subsets at ileum, bursa and cecal tonsil tissues.
Material and Methods: Thirty chickens were divided into three groups and fed with sterilized cow milk (M), or a mixture of M and LAB, kept as controls. Chickens from each group were sacrificed after 2 or 3 weeks and lymphoid tissues including ileum, bursa and cecal tonsil were removed and cryo preserved. The ratio of T cell subsets including CD4+, CD8+ and gamma delta T cells were studied by immune-histochemistry method.
Results: The ratio of CD4+ T cells was significantly higher in ileum of chicken after 2 weeks of probiotic intake. Moreover, this ratio was even higher than the ratio of these cells in the third week. In the cecal tonsil sections the percentage of gamma delta T cells was higher in milk fed chickens, after 3 weeks.
conclusion: The findings indicate that feeding of chickens with probiotics may alter the distribution of T cells in the lymphoid tissues. However, coincidental increase of CD4+ and gamma delta T cells after 2 and 3 weeks of treatment indicates that these effects are transient and could not have a permanent effect on avian mucosal immunity.
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Type of Study: Research Article | Subject: Physiology
Received: 2015/10/22 | Accepted: 2015/10/22 | Published: 2015/10/22

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