Aim and Background: Nowadays, using nanocarriers has increased in drug delivery industry. Niosome is one of the nanocarriers with non-ionic surfactant which increases the stability of drug in body. Since, using cisplatin in the treatment of cancers has some side effects, it was nanoniosomed.

Materials and Methods: Nanoniosomes were synthesized by reverse phase evaporation and ether injection methods. The mean diameter of particles was determined using Zetasizer. The encapsulation and drug loading efficiency of two formulations which were prepared from both reverse phase evaporation and ether injection methods was measured by spectrophotometric method. Drug release pattern was examined by dialysis in a period of 48 hours. Cytotoxicity effect on nanoniosomal cisplatin was determined on C6 cell line by MTT assay.

Results: The mean diameter of particles was synthesized by reverse phase evaporation and ether injection methods was determined 242.1±5.10 nm and 218.6±3.42 nm, respectively. The encapsulation and drug loading efficiency of two formulations were measured 43.6±4.59%, 52.8±3.22%; 4.36±0.08% and 5.28±0.17%, respectively. The results of drug release showed that released drug from prepared formulation using reverse phase evaporation was 97.53±0.55% and using ether injection was 81.23±1.24%. In addition, the results of cytotoxicity showed that the cytotoxicity of prepared formulation using ether injection more than prepared formulation applying reverse phase evaporation.

Conclusion: This study showed that using reverse phase evaporation to synthesize nanoniosomal cisplatin is more appropriate method than ether that of injection method.