Volume 7, Issue 25 (1-2017)                   NCMBJ 2017, 7(25): 85-90 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Bakhshi Khaniki G, Ali Nejad M, Kazemitabar S K, Hamidi R. An Investigation Of Genetic Diversity Urtica dioica L. in Central area of Mazandaran provenience by RAPD marker. NCMBJ 2017; 7 (25) :85-90
URL: http://ncmbjpiau.ir/article-1-922-en.html
Department of Biology and Microbiology, Islamic Azad University, Parand Branch ,Tehran, Iran , bakhshi@pnu.ac.ir
Abstract:   (8550 Views)
Aim and Background: Urtica dioica L. is used as medicinal plants to cure some disease such as; digestives disease or declined blood sugar and hypertension. It is known that by the increasing of population the confirmed need of pharmacy industries to medicinal plants as well as, the importance of their effective materials in nutritional adornment and hygienic are caused them to deserve special attention and research at the point of cultivation, production and consumption. In this research sample plants were collected central area of Mazandaran province was studied in aspect of genetic variation. Materials and Methods: In order to evaluate the genetic diversity, the genomic DNA was extracted by using modified CTAB protocol. The evaluation of quality and quantity of DNA was examined with agaros gel electrophoresis. Six RAPD markers have been used for PCR analysis. Results: Out of 155 RAPD bands, 68/38% were polymorphism. The data were analyzed with NTSYS programs. The dandrogram was drawn based on UPGMA. Conclusion: Investigation the genetic variation of this plant with RAPD indicated that this marker is suitable to determine the polymorphic loci and to estimate the genetic distance.
Full-Text [PDF 727 kb]   (2425 Downloads)    
Type of Study: Research Article | Subject: Genetics
Received: 2017/04/19 | Accepted: 2017/04/19 | Published: 2017/04/19

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | New Cellular and Molecular Biotechnology Journal

Designed & Developed by : Yektaweb