%0 Journal Article %A Goodarzi, Leila %A Yari, Reza %A Mirzaei, Mohsen %T dentifying antibiotic resistance genes in clinical isolates of Klebsiella pneumoniae producing IMP-1 and TEM β-lactamase %J New Cellularand Molecular Biotechnology Journal %V 9 %N 35 %U http://ncmbjpiau.ir/article-1-1201-en.html %R %D 2019 %K Klebsiella pneumonia, Extended-Spectrum Betalactamase (ESBL), TEM, IMP-1, Antibiotic Resistance, %X Aim &Background: Klebsiella pneumoniae is a common cause of β-lactamase-associated infections in hospitals. The present study aimed to determine the frequency of antibiotic resistance genes in Klebsiella pneumoniae strains producing IMP-1 and TEM β-lactamase. Materials and methods: The present research identified 94 samples of K. pneumoniae, using antibiogram for the phenotypic confirmation of ESBLs. The antibiotic resistance of the isolates and the prevalence of TEM and IMP-1 genes were determined using PCR method. Findings: Of 94 samples, 77.6% were ESBL-positive and 22.3% ESBL-negative. A total of 4.1% of the samples carried the IMP-1 gene and 43.8% the TEM gene, while 43.8% of the samples carried both genes. Conclusion: Given that TEM and IMP-1 genes were commonly present in a large number of the resistant samples, physicians are recommended to use therapeutic measures properly, and to prescribe antibiotics rationally. %> http://ncmbjpiau.ir/article-1-1201-en.pdf %P 25-34 %& 25 %! %9 Research Article %L A-10-61-78 %+ Department of Biology, Borujerd Branch, Islamic Azad University, Borujerd, Iran %G eng %@ 2228-5458 %[ 2019