fa جداسازی‌ سودوموناس آئروژینوزای مقاوم به آنتی‌بیوتیک دارای ژن bla_vim ا ز محیط‌های بیمارستانی میکروبیولوژی Microbiology مقاله پژوهشی Research Article <strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">سابقه و هدف</span></span></strong>: <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مصرف</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">گسترده</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">و</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بی</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">&shy;</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">رویه</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> آنتی&shy;بیوتیک&shy;ها، </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">دزانفکتانت</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">&shy;</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ها</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">وآنتی</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">&shy;</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سپتیک</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">&shy;</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ها</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">در</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مراکز</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">درمانی باعث</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">پیدایش</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مقاومت</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">در</span></span> <em><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سودوموناس</span></span></em> <em><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">آئروژنوزا</span></span></em><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> که </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">یکی</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">از</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مهم&shy;ترین</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">عوامل</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">عفونت</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">در</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بیمارستان می&shy;باشد، شده</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">است</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">. </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">هدف</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">از</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">این</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مطالعه</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بررسی</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> حضور </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ژن</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> &nbsp;</span></span><em><span dir="LTR"><span style="font-family:times new roman,serif;">bla_vim</span></span></em> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">در</span></span> <em><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سودوموناس</span></span></em> <em><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">آئروژنوزای</span></span></em> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مقاوم</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">به</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بتالاکتام</span></span><span dir="LTR"><span style="font-family:tahoma,sans-serif;"><span style="font-size:12.0pt;">&shy;</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ها از</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">محیط بیمارستان</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> بود.</span></span><br> <strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">مواد و</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">روش</span></span></strong><strong><span dir="LTR"><span style="font-family:italic;"><span style="font-size:16.0pt;">&shy;</span></span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">ها :</span></span></strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">120 </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سویه</span></span> <em><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سودوموناس</span></span></em> <em><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">آئروژنوزا</span></span></em> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">از</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">محیط</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">های</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بیمارستان</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">جمع</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">آوری</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">شد</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">. </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">حساسیت</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">این</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سویه</span></span><span dir="LTR"><span style="font-family:italic;"><span style="font-size:12.0pt;">&shy;</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ها</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نسبت به آنتی&rlm;بیوتیک&rlm;های بتالاکتام به روش دیسک گذاری سنجیده شد</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">. </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">حضور</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ژن </span></span><em><span dir="LTR"><span style="font-family:times new roman,serif;">bla_vim</span></span></em> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">با واکنش&rlm;های زنجیره&rlm;ای پلی&shy;مراز بررسی شد.</span></span><br> <strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">یافته &shy;ها</span></span></strong>: <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">در میان120 نمونه جداشده از محیط بیمارستان 50 نمونه <em>سودوموناس آئروژینوزا</em> بود.</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">در</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بین</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">باکتری</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">&shy;</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">های</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مورد</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">آزمایش</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">35 نمونه به آنتی&shy;بیوتیک&shy;های خانواده بتالاکتام&shy;ها مقاوم بودند که از میان آن&shy;ها 21 نمونه (42%) حاوی ژن </span></span><em><span dir="LTR"><span style="font-family:times new roman,serif;">bla_vim</span></span></em> <em><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بودند.</span></span></em><br> <strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">نتیجه&shy; گیری</span></span></strong>: <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نتایج</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">حاصل</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">از</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">این</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بررسی،</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">حضور</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ژن</span></span> <em><span dir="LTR"><span style="font-family:times new roman,serif;">bla_ vim</span></span></em> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">را</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">در</span></span> <em><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سودوموناس</span></span></em> <em><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">آئروژنوزا</span></span></em> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نشان</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">داد</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">. با </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مقایسه</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سایر</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">یافته</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">در</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سراسر</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">جهان،</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">افزایش</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">روز</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">افزون</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مقاومت</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ژنتیکی</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">باکتری</span></span> <em><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سودوموناس</span></span></em> <em><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">آئروژینوزا</span></span></em> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">را</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نسبت به آنتی</span></span><span dir="LTR"><span style="font-size:12.0pt;">&shy;</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بیوتیک</span></span><span dir="LTR"><span style="font-size:12.0pt;">&shy;</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ها به&shy; خصوص بتالاکتام&shy;ها </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مشخص</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">می</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">&shy;</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نماید</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">. </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">این</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نتایج برای کاربرد سیاست</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">&shy;</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">های</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">صحیح</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> استفاده از مواد ضد میکروبی </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">حائز</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">اهمیت</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">است</span></span><strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">.</span></span></strong> <strong>Aim and Background</strong>: Overplay of antibiotics in clinical centers not only did not destroy pathogenic bacterias but also grew a resistance for these kind of compounds in microorganisms such as <em>Pseudomonas aeruginosa</em> that is one of important infection factors in hospitals. The purpose of this study is to investigate the resistant gene to antibiotic,<em> bla_vim</em> gene<em>, </em>which was found among <em>Pseudomonas aeruginosas</em> released from hospitals.<br> <strong>Materials and Methods</strong>: 120 strains of<em> Pseudomonas aeruginosas </em>were collected from hospitals. The sensitivity of these strains to antibiotics was determined by Disc diffusion test. PCR was used for identification of<em> bla_vim </em>gene <em>.</em><br> <strong>Results</strong>: Among 120 samples isolated from hospitals 50 cases were <em>Pseudomonas</em> <em>aeruginosa</em>. 35 <em>Pseudomonas aeruginosa </em>samples were resistant to antibiotics. 21 samples (42%) of 35 samples contained <em>bla_vim</em> gene.<br> <strong>Conclusion</strong>: The results of this study indicated the presence of <em>bla</em><em> _vim</em> gene in <em>Pseudomonas aeruginosa.</em> The comparison of the results with the rest of the world indicates the increasing genetic resistance of <em>Pseudomonas aeruginosa </em>to antibiotics especially beta-lactams. These results are important in taking proper health policies and using effective antibiotics <em>for</em> <em>hospital infections</em><em>.</em> سودوموناس آئروژینوزا, مقاومت به آنتی‌بیوتیک, ژن bla_ vim , بتالاکتام‌ها. Pseudomonas aeruginosa, antibiotic resistance, bla _vim gene, beta-lactams 97 106 http://ncmbjpiau.ir/browse.php?a_code=A-10-954-14&slc_lang=fa&sid=1 Elham Siasi الهام سیاسی emi_biotech2006@yahoo.ca 10031947532846007959 10031947532846007959 Yes Department of Microbiology, School of science, Islamic Azad University, North Tehran Branch, Tehran, Iran گروه میکروبیولوژی، دانشکده علوم زیستی، دانشگاه آزاد اسلامی ، واحد تهران شمال ، تهران ، ایران Robab Rafiei Tabatabaii رباب رفیعی طباطبایی 10031947532846007960 10031947532846007960 No Department of Microbiology, School of science, Islamic Azad University, North Tehran Branch, Tehran, Iran گروه میکروبیولوژی، دانشکده علوم زیستی، دانشگاه آزاد اسلامی ، واحد تهران شمال ، تهران ، ایران Fatemeh MoslehiMehr فاطمه مصلحی مهر 10031947532846007961 10031947532846007961 No Department of Microbiology, School of science, Islamic Azad University, North Tehran Branch, Tehran, Iran گروه میکروبیولوژی، دانشکده علوم زیستی، دانشگاه آزاد اسلامی ، واحد تهران شمال ، تهران ، ایران