fa بررسی ارتباط فراوانی اینتگرون کلاس I و مقاومت دارویی در نمونه‌های بالینی جدایه‌های سودوموناس آئروژینوزا سلولی و مولکولی Cellular and molecular مقاله پژوهشی Research Article <strong><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">سابقه و هدف</span></span></span></strong>: <em><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">Pseudomonas aeroginosa</span></span></span></em> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">یکی</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> از مهم&shy;ترین عوامل عفونت&shy;ها</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ی</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> بیمارستانی است. </span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">این</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مطالعه</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">با</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">هدف</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بررسی</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">فراوانی</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">اینتگرون</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">کلاس</span></span></span> <span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">I</span></span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">&nbsp;در</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ایزو</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">له &shy;ها</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ی</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> بالینی </span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سودوموناس آئروژینوزا</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">&nbsp;و ارتباط آن </span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">با</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">الگوی</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مقاومت</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">آنتی&shy; بیوتیکی</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">انجام</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">شد</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:b zar;"><span style="font-size:12.0pt;">.</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">&nbsp; &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></span></span><br> <strong><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">مواد و روش &shy;ها:</span></span></span></strong><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> 103 سویه سودوموناس</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">آئروژینوزا</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">جدا شده از نمونه&shy;ه ای بالینی مورد استفاده قرار گرفت. </span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ایزوله&shy; ها</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">از نظر حضور</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">اینتگرون</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">کلاس </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">I</span></span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">و ارتباط بین</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">حضور</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">اینتگرون</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">و</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">الگوی</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مقاومت</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">آنتی&shy; بیوتیکی</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">با</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">آزمون</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">2</span></span></span><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">&chi;</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">در</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> سطح معنی&shy;داری 05/0 بررسی شدند. </span></span></span><br> <strong><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">یافته &shy;ها</span></span></span></strong><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">:</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> بیش&shy;ترین مقاومت مربوط به سفوتاکسیم (7/75%) و بیش&shy;ترین حساسیت </span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">به سفتازیدیم-کلاولانیک (7/73%) بود. 7/40% ایزوله&shy; ها</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">دارای</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">اینتگرون</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بوده و ارتباط</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">معنی</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-size:12.0pt;">&shy;</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">داری</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بین</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">حضور</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">اینتگرون</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">کلاس</span></span></span> <span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">I</span></span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">&nbsp;و مقاومت به اغلب آنتی&shy; بیوتیک&shy; ها مشاهده گردید.</span></span></span><br> <strong><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">نتیجه&shy; گیری</span></span></span></strong>: <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">با</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">توجه</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">به فراوانی بالای</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">اینتگرون</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">کلاس</span></span></span> <span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">I</span></span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">در</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ایزوله</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-size:12.0pt;">&shy;</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">های</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سودوموناس آئروژینوزا</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">و</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ارتباط</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">آن</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">با</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مقاومت</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">دارویی؛</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">اعمال</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">راهکارهای</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مناسب</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">کنترل</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">عفونت</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">و</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">درمان</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">در</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بیمارستان</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-size:12.0pt;">&shy;</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ها برای</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">جلوگیری</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">از</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">انتشار</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بیش&shy;تر آن&shy;ها</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ضروری</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">به&shy; نظر می</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-size:12.0pt;">&shy;</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">رسد.</span></span></span> <strong>Aims and Background</strong>:<br> &nbsp;<em>Pseudomonas aeruginosais</em> is one of the most important pathogens for nosocomial infections, particularly in ICU and burn. This bacterium causes diseases such as respiratory infections, sepsis and bacteremia. Since the serious infections caused by Pseudomonas resistance against conventional antibiotics is a serious problem and due to the placement of genes coding for antibiotic resistance on their Integrons and quick release feature identification of strains containing Integrons provide useful information on the extent and development of drug resistance. The aim of this study was to evaluate the frequency of class I integrons in <em>Pseudomonas aeruginosa</em> strains isolated from clinical samples and their relationship to antibiotic resistance.<br> <strong>Materials and Methods</strong>:<br> 103 strains of<em> Pseudomonas aeruginosa</em> isolated from different clinical specimens were used. The drug susceptibility test, using 12 antimicrobial agents, was performed for all the isolates via agar disk diffusion method. The specific PCR was used to identify class I integrons and the relationship between class I integrons presence and antibiotic resistance was evaluated by &chi;2 test.<br> <strong>Results</strong>:<br> Analysis of antibiotic resistance patterns showed 75.7% of the isolates were resistant to cefotaxime and 73.7% of the isolates were sensitive to ceftazidime - clavulanic. The PCR results showed that 40.7% of isolates have class I integron. A significant correlation was obtained between the presence of class I integrons and resistance against most common antibiotics (P <0.05).<br> <strong>Conclusion</strong>:<br> Due to the high frequency of class I integrons and connection with drug resistance in <em>Pseudomonas aeruginosa</em> isolates, apply appropriate strategies for infection control and hospital treatment to prevent further spread is essential. سودوموناس آئروژینوزا, مقاومت آنتی‌بیوتیکی, اینتگرون Pseudomonas aeruginosa, antibiotic resistance, Integrons 49 56 http://ncmbjpiau.ir/browse.php?a_code=A-10-61-77&slc_lang=fa&sid=1 Reza Yari رضا یاری rezayari@yahoo.com 10031947532846008529 10031947532846008529 Yes Department of Biology, Faculty of Sciences, Borujerd Branch, Islamic Azad University, Borujerd, Iran 1- گروه زیست شناسی، دانشکده علوم، واحد بروجرد، دانشگاه آزاد اسلامی، بروجرد، ایران. Mohammad Reza Mehrabi محمدرضا مهرابی 10031947532846008530 10031947532846008530 No Department of Laboratory Sciences, Medical Faculty, Borujerd Branch, Islamic Azad University, Borujerd, Iran. گروه علوم آزمایشگاهی، دانشکده علوم پزشکی، واحد بروجرد، دانشگاه آزاد اسلامی، بروجرد، ایران. Akram Mahdipour اکرم مهدی پور 10031947532846008531 10031947532846008531 No Department of Biology, Faculty of Sciences, Borujerd Branch, Islamic Azad University, Borujerd, Iran 1- گروه زیست شناسی، دانشکده علوم، واحد بروجرد، دانشگاه آزاد اسلامی، بروجرد، ایران. Shahram Nakhjavan شهرام نخجوان 10031947532846008532 10031947532846008532 No Department of Agronomy and Plant Breeding, Borujerd Branch, Islamic Azad University, Borujerd, Iran 3- گروه اصلاح نباتات، دانشکده کشاورزی، واحد بروجرد، دانشگاه آزاد اسلامی، بروجرد، ایران.