New Cellularand Molecular Biotechnology Journal
مجله تازه هاي بيوتكنولوژي سلولي و مولكولي
NCMBJ
Basic Sciences
http://ncmbjpiau.ir
1
admin
2228-5458
2228-6926
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8888
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fa
jalali
1397
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1
gregorian
2018
12
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33
online
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fulltext
fa
اثر آب تهی شده از دوتریم بر روی رشد سلولی و سیستم آنتی اکسیدانی سلول های سرطان سینه
The effect of deuterium depleted water on cell growth and antioxidant systems of breast cancer cells
سلولی و مولکولی
Cellular and molecular
مقاله پژوهشی
Research Article
<strong><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">سابقه و هدف</span></span></span></strong><strong><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">:</span></span></span></strong> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مطالعه­های اخیر نشان می­ دهد که کاهش </span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">طبیعی </span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">میزان دوتریم می­تواند منجر به مهار رشد تومور در هر دو مدل</span></span></span> <span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">in vitro</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">و</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"> in vivo</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">شود. هدف از </span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">این مطالعه</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> بررسی اثر آب</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">تهی</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">شده</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">از</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">دوتریوم</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:13.0pt;"> (</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">DDW</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:13.0pt;">)</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">تنها و در ترکیب با </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">5-FU</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بر</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> روی </span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">رشد و سیستم آنتی اکسیدانی سلول</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">­</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">های سرطان سینه است. </span></span></span><br>
<strong><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">مواد و روش­ ها</span></span></span></strong><strong><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">:</span></span></span></strong> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">آزمایش </span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سمیت سلولی با استفاده از روش </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">MTT</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> انجام شد. سلول</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">­</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">های </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">MCF-7</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> با غلظت های مختلفی از </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">DDW</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> تنها، </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">5-FU</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> تنها و هر دو تیمار شدند. سطح </span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مالون دی الدئید و فعالیت آنزیم</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">­</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">های کاتالاز و سوپر اکسید دیسموتاز به روش اسپکتروفتومتری انجام گردید. </span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">آنالیز </span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">واریانس یک</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">­</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">طرفه </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"> (ANOVA)</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">و </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">t-Test</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> برای</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> تجزیه و تحلیل آماری استفاده شدند. </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">P ≤ 0.05</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> سطح معنی­داری در نظر گرفته شد.</span></span></span><br>
<strong><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">یافته ­ها:</span></span></span></strong><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> تیمار سلول</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">­</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ها با غلظت­های مختلف </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">DDW</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> تنها به­ویژه در غلظت­های 100 و </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">ppm</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> 125 باعث مهار رشد سلول</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">­</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">های</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">MCF-7</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> گردید. </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">5-FU</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> تنها نیز به</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">­</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">طور قابل توجهی میزان بقاء سلول</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">­</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">های </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">MCF-7</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> را کاهش داد و استفاده از </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">DDW</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> به همراه </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">5-FU</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> اثر مهاری </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">5-FU</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> بر روی سلول</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">­</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ها را افزایش داد. در مقایسه با گروه کنترل (</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">ppm</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> 150)، مشاهده گردید که در سلول</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">­</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">های تیمار شده با غلظت­های </span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">پایین</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">­</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">تر</span></span></span> <span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">DDW</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">، فعالیت آنزیم</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">­</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">های کاتالاز و سوپراکسید دیسموتاز افزایش ولی سطح مالونیل دی آلدهید کاهش یافته است. </span></span></span><br>
<strong><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">نتیجه­ گیری:</span></span></span></strong> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">آب تهی شده از دوتریوم می</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;">­</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">تواند به عنوان یک کمک کننده در داروهای ضد سرطان در نظر گرفته شود. </span></span></span><br>
<strong>Aim and Background:</strong><br>
Recent studies show that the depletion of naturally occurring deuterium can result in tumor regression in both in vitro and in vivo models. The purpose of this study was to investigate the effect of deuterium depleted water (DDW) discretely and in combination with 5-FU on cell growth and anti-oxidant system of breast cancer cells.<br>
<strong>Mateial and Methods:</strong><br>
The cellular cytotoxicity test was performed using MTT assay. The MCF-7 cells were treated with decreasing deuterium concentrations of DDW alone, 5-FU alone and both. The malondialdehyde (MDA) level and the catalase (CAT) and superoxide dismutase (SOD) enzyme activities were identified spectrophometrically. One way analysis of variance (ANOVA) and t- test were used for statistical analysis. P ≤ 0.05 was considered significant.<br>
<strong>Results:</strong> Treatment the cells with different concentrations of DDW alone especially in concentrations of 100 and 125ppm imposed growth inhibitory effect on MCF-7 cells. Also, 5-FU alone significantly decreased the survival fractions of MCF-7 cells and DDW in combination with 5-FU augmented 5-FU inhibitory effects on the cells. Compared to the control group (150 ppm), it was observed that in the cells treated with Lower concentrations of DDW, the activities of catalase and superoxide dismutase enzymes increased, but the level of malonyldialdehyde decreased.<br>
<strong>Conclusion</strong><strong>:</strong> Deuterium depleted water can be considered as an adjuvant in anticancer drugs.<br>
آب تهی شده از دوتریوم, سرطان سینه, آنزیمهای آنتی اکسیدان, مهار رشد سلولی, MTT
Deuterium Depleted Water, Breast Cancer, Anti-Oxidants Enzymes, Cell Growth Inhibition, MTT
87
98
http://ncmbjpiau.ir/browse.php?a_code=A-10-336-3&slc_lang=fa&sid=1
kamal
yavari
کمال
یاوری
kyavari@aeoi.org.ir
100319475328460011052
100319475328460011052
Yes
پژوهشگاه علوم و فنون هسته ای، تهران، ایران
lida
koshesh
لیدا
کوشش
100319475328460011053
100319475328460011053
No
دانشکده علوم زیستی، واحد تهران شمال ، دانشگاه آزاد اسلامی، تهران ،ایران
Monireh
Movahedi
منیره
موحدی
100319475328460011054
100319475328460011054
No
دانشکده علوم زیستی، واحد تهران شمال ، دانشگاه آزاد اسلامی، تهران ،ایران