New Cellularand Molecular Biotechnology Journal
مجله تازه هاي بيوتكنولوژي سلولي و مولكولي
NCMBJ
Basic Sciences
http://ncmbjpiau.ir
1
admin
2228-5458
2228-6926
-
-
-
8888
-
fa
jalali
1398
9
1
gregorian
2019
12
1
10
37
online
1
fulltext
fa
ارتباط پلیمورفیسم rs10573247 در 3ˈ-UTR ژن HMGA2 با خطر ابتلا به سرطان معده
The association of rs10573247 polymorphism in 3ˈ-UTR of HMGA2 gene with risk of gastric cancer
سلولی و مولکولی
Cellular and molecular
مقاله پژوهشی
Research Article
<strong><span style="font-family:B Nazanin;"><span style="font-size:16.0pt;">چکیده</span></span></strong><br>
<strong><span style="font-family:B Nazanin;"><span style="font-size:16.0pt;">سابقه وهدف: </span></span></strong><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">پروتئین </span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:11.0pt;">HMGA2</span></span></span></em><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> یکی از فاکتورهای رونویسی تنظیم کننده تکامل و تمایز سلول است. بررسی</span></span><strong><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:11.0pt;">­</span></span></span></strong><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">ها نشان میدهد که تغییرهای بیان ژن </span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:11.0pt;">HMGA2</span></span></span></em><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> می</span></span><strong><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:11.0pt;">­</span></span></span></strong><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">تواند نقش مهمی در جلوگیری از پیشرفت تومور و جلوگیری از متاستاز آن داشته باشد.</span></span><br>
<strong><span style="font-family:B Nazanin;"><span style="font-size:16.0pt;">مواد و روش</span></span></strong><span dir="LTR"><span style="font-family:Times New Roman,serif;">­</span></span><strong><span style="font-family:B Nazanin;"><span style="font-size:16.0pt;">ها:</span></span></strong> <span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">از نمونه خون 100 فرد مبتلا به سرطان معده و 100 فرد سالم </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;">DNA</span></span> <span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> ژنومی استخراج شد. سپس بخشی از ژن </span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;">HMGA2</span></span></em><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> که</span></span> <span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">در برگیرنده پلی­ مورفیسم </span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;">rs10573247</span></span></em> <span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">بود با تکنیک </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;">PCR</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> تکثیر شد. در ادامه محصول </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;">PCR</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> با آنزیم محدودالاثر</span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;">EaR1 </span></span></em><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> تیمار شد و ژنوتیپ هر فرد مشخص </span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">گردید</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">. در نهایت با تست آماری <sup>2</sup></span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;">χ</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> و رگرسیون لجستیک ارتباط این واریانت با خطر ابتلا به بیماری سرطان معده بررسی شد.</span></span><br>
<strong><span style="font-family:B Nazanin;"><span style="font-size:16.0pt;">یافته</span></span></strong><span dir="LTR"><span style="font-family:Times New Roman,serif;">­</span></span><strong><span style="font-family:B Nazanin;"><span style="font-size:16.0pt;">ها:</span></span></strong> <span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">بررسی آماری ارتباط آللی و ژنوتیپی پلی­ مورفیسم </span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;">rs10573247</span></span></em> <span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">با خطر ابتلا به سرطان معده نشان داد که ارتباط معناداری بین آلل</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;">­</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">های مختلف پلی­ مورفیسم </span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;">rs10573247</span></span></em><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> (127/0</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;">P =</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">) و هیچ یک از ژنوتیپ­ های آن با خطر ابتلا به سرطان معده وجود ندارد. </span></span><br>
<strong><span style="font-family:B Nazanin;"><span style="font-size:16.0pt;">نتیجه­ گیری</span></span></strong><span style="font-family:B Nazanin;"><span style="font-size:16.0pt;">:</span></span> <span style="font-family:B Nazanin;"><span style="font-size:12.0pt;">عدم ارتباط پلی­ مورفیسم </span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;">rs10573247</span></span></em><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> در ژن </span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;">HMGA2</span></span></em><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"> با خطر ابتلا به سرطان معده ارتباطی ندارد که می­تواند ناشی از حجم کم نمونه مورد بررسی باشد.</span></span><span style="font-family:B Nazanin;"><span style="font-size:12.0pt;"></span></span>
<strong>Aim and Background</strong><strong>:</strong> The HMGA2 protein is one of the transcription factors that regulates the evolution and differentiation of the cells. Studies have shown that changes in the expression of the HMGA2 gene can play an important role in preventing tumor progression and its metastasis. In this study, we investigated the association between rs10573247 polymorphism in the 3-UTR region of HMGA2 gene and the risk of gastric cancer.<br>
<strong>Material and Methods:</strong> Genomic DNA from blood sample of 100 patients with gastric cancer and 100 healthy individuals were extracted A part of the HMGA2 gene, including rs10573247 polymorphism, was amplified by PCR technique. After, the PCR product was treated with EaR1 enzyme and the genotype of each individual was determined. Finally, the risk of gastric cancer was assessed with the statistical tests of χ2 and logistic regression.<br>
<strong>Results:</strong> The statistical analysis of allelic and genotypic association of rs10573247 polymorphism with the risk of gastric cancer showed that there was not significant relationship between different alleles of rs10573247 polymorphism (P = 0.127) and none of its genotypes with gastric cancer risk.<br>
<strong>Conclusion</strong><strong>:</strong> It seems none of the rs10573247 polymorphism genotypes in the HMGA2 gene were associated with the risk of gastric cancer.
سرطان معده, پلیمورفیسم rs10573247, ژن , HMGA2,IAU-science
Gastric Cancer, Polymorphism rs10573247, HMGA2 gene,IAU-science
9
20
http://ncmbjpiau.ir/browse.php?a_code=A-10-1383-3&slc_lang=fa&sid=1
Hanie
Heidari
هانیه
حیدری
100319475328460010910
100319475328460010910
Yes
Department of biology, Faculty of Science, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
گروه زیست شناسی، دانشکده علوم پایه، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران
Parisa
Mohamadynejad
پریسا
محمدی نژاد
parisa_mohamadynejad@yahoo.com
100319475328460010911
100319475328460010911
No
Department of biology, Faculty of Science, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
گروه زیست شناسی، دانشکده علوم پایه، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران