fa افزایش بیان ژنB7-H4 درسرطان معده و نقش آن بعنوان یک بیومارکر بالقوه تشخیصی سلولی و مولکولی Cellular and molecular مقاله پژوهشی Research Article <strong><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">سابقه و هدف</span></span></strong>: <span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">سرطان معده یکی از متداولترین سرطانهای شایع با مرگ و میر بالاست. </span></span></span><em><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">B7-H4</span></span></span></span></em> <span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">بعنوان یک </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">بیومارکر جدید تشخیصی </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">بالقوه</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> در </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">سرطان معده</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> ذکر شده است</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">. اما اهمیت بالینی آن در این زمینه هنوز نامشخص است</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">. هدف از مطالعه حاضر، بررسی </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">سطح</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> بیان </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">mRNA</span></span></span></span> <span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">ژن </span></span></span><em><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">B7-H4</span></span></span></span></em> <span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">در نمونه&shy;های توموری</span></span></span> <span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">متاستازی و غیرمتاستازی در مقایسه با حاشیه تومور بافتهای مربوطه و ارزیابی نقش آن </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:10.0pt;">در</span></span></span> <span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">پیشرفت و پیش&shy;آگهی تومور بود.</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"></span></span></span><br> <strong><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">مواد و روشها</span></span></strong><strong><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">:</span></span></span></strong><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> در این مطالعه، </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">بلوکهای پارافینه از60 بیمار با گاستریک آدنوکارسینوما ( 30 مورد همراه با متاستاز در زمان تشخیص و30 مورد بدون شواهد متاستاز) </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">که طی </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">سالهای 1387 تا</span></span></span> <span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">1397 در بیمارستان امام خمینی تهران تحت گاستروکتومی قرار گرفته بودند،</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> مورد </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">استفاده قرار گرفت. </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">نمونه&shy;ها به روش پانچ ( شامل تومور و حاشیه تومور برای هر نمونه) از بلوکهای پارافینه بعد از مشخص شدن ناحیه مد نظر توسط پاتولوژیست گرفته شد.</span></span></span> <span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">استخراج </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">RNA</span></span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> از بخشهای توموری و حاشیه تومور انجام شد. بیان </span></span></span><em><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">B7-H4</span></span></span></span></em> <span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">mRNA</span></span></span></span> <span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">از طریق</span></span></span> <span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">qRT-PCR</span></span></span><span style="color:black;"><span style="font-family:B Lotus;">مورد ارزیابی قرار گرفت.</span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"></span></span></span><br> <strong><span style="color:#242021;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">یافته &shy;ها: </span></span></span></strong><span style="color:#242021;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">افزایش بیان </span></span></span><em><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">B7-H4</span></span></span></span></em> <span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">mRNA</span></span></span></span> <span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">در </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">5</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">/81% از بافتهای توموری </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">مشاهده شد. </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">اختلاف </span></span></span><span style="color:#242021;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">معنی&shy;دار در بیان </span></span></span><em><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">B7-H4</span></span></span></span></em> <span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">mRNA</span></span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> در </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">بافتهای توموری موارد همراه با متاستاز </span></span></span><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">(001/0></span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">p</span></span></span></em><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">)</span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"> و</span></span></span> <span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">بدون متاستاز </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">(003</span></span></span><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">/0></span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">p</span></span></span></em><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">) </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">در مقایسه با حاشیه تومور دیده شد. </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">اما مقایسه </span></span></span><strong><span style="color:#242021;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">بیان </span></span></span></strong><em><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">B7H4</span></span></span></span></em> <span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">mRNA</span></span></span></span> <span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">بین بافتهای توموری افراد با متاستاز یا بدون &shy;متاستاز، تفاوت معنی&shy;داری </span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">(</span></span></span><span style="font-family:Times New Roman,serif;">0.732</span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;">p</span></span></em><span dir="LTR"><span style="font-family:Times New Roman,serif;">=</span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">) را نشان نداد.</span></span></span> <span style="font-family:B Lotus;"><span style="font-size:12.0pt;">همچنین </span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">آنالیزها ارتباط معنی&shy;دار بین بیان </span></span></span><em><span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">B7-H4</span></span></span></span></em> <span dir="LTR"><span style="color:black;"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">mRNA</span></span></span></span> <span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">و متغیرهای کلینیکال وهسیتوپاتولوژی را نشان نداد.</span></span></span><span style="color:black;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"></span></span></span><br> <strong><span style="color:#242021;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">نتیجه گیری: </span></span></span></strong><span style="font-family:B Lotus;"><span style="font-size:12.0pt;">یافته&shy;های ما حاکی از نقش احتمالی </span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:10.0pt;">B7-H4</span></span></span></em> <span style="font-family:B Lotus;"><span style="font-size:12.0pt;">بعنوان یک بیومارکر، در بیماران با سرطان معده است. اگر چه تحقیقات بیشتر در این زمینه مورد نیاز است. </span></span><strong><span style="color:#242021;"><span style="font-family:B Lotus;"><span style="font-size:12.0pt;"></span></span></span></strong> <strong>Aims and </strong><strong>Background:</strong> Gastric cancer is one of the most common cancers with high mortality. <em>B7-H4</em> has been identified as potential novel diagnostic biomarker in gastric cancer. But, its clinical significance in this area is still unclear. The purpose of the present study was to examine the expression level of <em>B7-H4</em> mRNA in tumor tissue of gastric cancer patient with and without history in comparison with their marginal tissue. Also we evaluate its role in tumor progression and prognosis. &nbsp;<br> <strong>Materials and Methods</strong>: In this study, paraffin blocks were studied from 60 patients with gastric adenocarcinoma (30 with metastasis at the time of diagnosis and 30 without evidence of metastasis) who underwent gastrectomy during 1387-97 in Imam Khomeini Hospital in Tehran. Samples were taken from paraffin blocks by punching method (including tumor and tumor margin for each sample) after the area was identified by the pathologist. RNA extraction was performed from tumor sections and tumor margins. <em>B7-H4</em> mRNA expression was assessed by quantitative Real-time polymerase chain reaction (qRT-PCR)<span dir="RTL">.</span><br> <strong>Results: </strong>Increased expression of <em>B7-H4</em> mRNA was observed in 81.5% of tumor tissues. There was a significant difference in <em>B7-H4</em> mRNA expression in tumor tissues of cases with metastasis (p <0.001) and without metastasis (p <0.003) compared to tumor margin. However, comparison of <em>B7-H4</em> mRNA expression between tumor tissues of metastatic and non-metastatic individuals did not show a significant difference (p = 0.732). Analyzes did not show a significant relationship between B7-H4 mRNA expression and clinical and histopathological variables.<br> <strong>Conclusion:</strong> Our findings indicate the possible role of B7-H4 as a potential diagnostic biomarker in patients with gastric cancer. Although more research is needed in this area.<br> &nbsp; سرطان معده, qRT-PCR , B7-H4 و Iau Science. Gastric cancer, B7H4, qRT-PCR, Iau Science. 83 90 http://ncmbjpiau.ir/browse.php?a_code=A-10-1654-2&slc_lang=fa&sid=1 Khadijeh Fanaei خدیجه فنائی 100319475328460012111 100319475328460012111 No Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran گروه زیست شناسی، دانشکده علوم پایه، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات، تهران، ایران. Iman Salahshourifar ایمان سلحشوری فر isalahshouri@gmail.com 100319475328460012112 100319475328460012112 No Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran گروه زیست شناسی، دانشکده علوم پایه، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات، تهران، ایران. Shiva Irani شیوا ایرانی 100319475328460012113 100319475328460012113 Yes Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran گروه زیست شناسی، دانشکده علوم پایه، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات، تهران، ایران. Mohsen Esfandbod محسن اسفند بد 100319475328460012114 100319475328460012114 No Department of Hematology and Oncology, Imam Khomeini Hospital complex, Tehran University of Medical Sciences TUMS. گروه هماتولوژی و انکولوژی، مجتمع بیمارستانی امام خمینی، دانشکده علوم پزشکی دانشگاه تهران. Fereshteh Ameli فرشته عاملی 100319475328460012115 100319475328460012115 No Pathology Department, Cancer Institute, Imam Khomeini Hospital complex, Tehran University of Medical Sciences گروه پاتولوژی، انستیتو کانسر، مجتمع بیمارستانی امام خمینی، دانشکده علوم پزشکی دانشگاه تهران.