fa ارزیابی تأثیر نانوکامپوزیت‌ مبتنی بر کربن‌کوانتوم‌دات حاوی زنیان بر بیان ژن gtf در استرپتوکوکوس موتانس میکروبیولوژی Microbiology مقاله پژوهشی Research Article <br> <br> <span style="font-size:11pt"><span style="background:white"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">سابقه و هدف:</span></span></span></b><span style="background-color:#ffffff;"> </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">باکتری</span></span></span><i><span style="background-color:#ffffff;"> </span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">استرپتوکوکوس</span></span></span></i><i><span style="background-color:#ffffff;">&shy;</span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">موتانس</span></span></span></i><span style="background-color:#ffffff;"> </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">عامل</span></span></span><span style="background-color:#ffffff;"> </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">اصلی</span></span></span><span style="background-color:#ffffff;"> </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">ایجاد</span></span></span><span style="background-color:#ffffff;"> </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">پوسیدگی </span><span style="background:yellow"><span style="background-color:#ffffff;">دندان</span></span></span></span><span style="background-color:#ffffff;"> </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">است.</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;"> ایجاد بیوفیلم&shy;های باکتری مذکور منوط به حضور آنزیم گلوکوزیل ترانسفراز است که توسط </span><span style="background:yellow"><span style="background-color:#ffffff;">ژن</span></span></span></span><span style="background-color:#ffffff;">&nbsp;</span><span dir="LTR" lang="EN" style="background:yellow"><span style="color:#202122"><span style="background-color:#ffffff;">Glycosyltransferase</span></span></span><i><span style="background-color:#ffffff;"> </span></i><span dir="LTR" style="background:yellow"><span style="background-color:#ffffff;">(</span><i><span style="background-color:#ffffff;">gtf</span></i><span style="background-color:#ffffff;">)</span></span><i><span style="background-color:#ffffff;"> </span></i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">کدگذاری می&shy;شود. هدف از این </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">مطالعه </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">ارزیابی تأثیر</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;"> نانوکامپوزیت پلی&shy;ساکاریدی</span></span></span><span style="background-color:#ffffff;"> </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">زیستی</span></span></span><span style="background-color:#ffffff;"> </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">زنیان/</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">کیتوزان/کربوکسی&shy;متیل&shy;نشاسته/کربن&shy;کوانتوم&shy;دات-منیزیم </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="background-color:#ffffff;">Trachyspermum</span></span><span dir="LTR" style="font-size:10.0pt"><span style="background-color:#ffffff;">-Cs-CMS-CQD.Mg)</span></span><b><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">) </span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">بر میزان </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">بیان ژن‌های </span></span></span><span style="background-color:#ffffff;">&nbsp;</span><i><span style="background-color:#ffffff;">gtfD</span></i><i><span style="background-color:#ffffff;">, </span></i><i><span style="background-color:#ffffff;">gtfC</span></i><span style="background-color:#ffffff;">, </span><i><span style="background-color:#ffffff;">gtfB</span></i><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;"> در </span><i><span style="background-color:#ffffff;">استرپتوکوکوس موتانس</span></i></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;"> با استفاده از روش </span></span></span><span style="background-color:#ffffff;">&nbsp;Real Time-PCR</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">در تشکیل بیوفیلم دندانی است.</span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="background:white"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">مواد و روش&shy;ها</span></span></b><b><span dir="LTR" style="font-size:16.0pt">:</span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">پس از تهیه نانوکامپوزیت</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">زنیان/</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">کیتوزان/کربوکسی&shy;متیل&shy;نشاسته/کربن&shy;کوانتوم&shy;دات-من<span style="background-color:#ffffff;">یزیم، </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">اثر</span></span></span><span style="background-color:#ffffff;"> </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">ضدمیکروبی آن&shy; روی باکتری </span><i><span style="background-color:#ffffff;">استرپتوکوکوس</span></i></span></span><i><span style="background-color:#ffffff;">&shy;&nbsp;</span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">موتانس</span></span></span></i><span style="background-color:#ffffff;"> ATCC 35668</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">با روش میکرودایلوشن بررسی گردید. مورفولوژی</span></span></span><span style="background-color:#ffffff;"> </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">و</span></span></span><span style="background-color:#ffffff;"> </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">خصوصیات</span></span></span><span style="background-color:#ffffff;"> </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">ساختاری نانوکامپوزیت&shy; توسط </span><span style="background:yellow"><span style="background-color:#ffffff;">طیف سنجی&shy; مادون قرمز </span></span></span></span><span dir="LTR" style="background:yellow"><span style="color:#202124"><span style="background-color:#ffffff;">Fourier Transform Infrared Spectroscopy&nbsp;</span></span></span><span dir="LTR" style="background:yellow"><span style="background-color:#ffffff;">(FTIR</span></span><span style="background-color:#ffffff;">)</span><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;"> و</span><span style="background:yellow"><span style="background-color:#ffffff;"> میکروسکوپ الکترونی روبشی</span></span><span style="background-color:#ffffff;"> </span></span><span style="background-color:#ffffff;">(SEM) </span><b><span style="background-color:#ffffff;">&nbsp;</span></b><span dir="LTR" style="background:yellow"><span style="color:#202124"><span style="background-color:#ffffff;">Scanning Electron Microscop</span></span></span><span dir="LTR" style="background:white"><span style="color:#202124"><span style="background-color:#ffffff;">y</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">بررسی</span></span></span><span style="background-color:#ffffff;"> </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">گردید.</span></span></span><i><span style="background-color:#ffffff;"> </span></i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">بیان &shy;ژن‌های</span></span></span><span style="background-color:#ffffff;"> </span><i><span style="background-color:#ffffff;">gtfD</span></i><i><span style="background-color:#ffffff;">, </span></i><i><span style="background-color:#ffffff;">gtfC</span></i><i><span style="background-color:#ffffff;">, </span></i><i><span style="background-color:#ffffff;">gtfB</span></i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">با تکنیک</span></span></span><span dir="LTR" style="background:yellow"><span style="background-color:#ffffff;">Real time-Polymerase Chain Reaction</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">مورد بررسی قرار گرفت</span></span></span><span style="background-color:#ffffff;">.</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;"> تحلیل</span></span></span><span style="background-color:#ffffff;"> </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">داده &shy;ها</span></span></span><span style="background-color:#ffffff;"> </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">با</span></span></span><span style="background-color:#ffffff;"> </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">نرم</span></span></span><span style="background-color:#ffffff;">&shy;</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">افزار </span></span></span><span style="background-color:#ffffff;">&nbsp;SPSS</span>_{<span style="background-color:#ffffff;">22</span>}<span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">و مقایسه</span></span></span><span style="background-color:#ffffff;"> </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">میانگین &shy;ها</span></span></span><span style="background-color:#ffffff;"> </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">براساس </span></span></span><span style="background-color:#ffffff;">(ANOVA) &nbsp;</span><span dir="LTR" style="background:yellow"><span style="background-color:#ffffff;">Analysis of variance</span></span><span style="background-color:#ffffff;">&nbsp;</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">در سطح</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="background:yellow"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;"> اطمینان</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;"> 05/0 انجام شد.</span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="background:white"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">یافته&shy;ها</span></span></span></b><b><span dir="LTR" style="font-size:16.0pt"><span style="background-color:#ffffff;">:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">اندازۀ</span></span></span><span style="background-color:#ffffff;"> </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">ذرات</span></span></span><span style="background-color:#ffffff;"> </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">نانوکامپوزیت</span></span></span><span style="background-color:#ffffff;"> </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">زنیان/</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="background-color:#ffffff;">کیتوزان/کربوکسی &shy;متیل&shy; نشاسته/کربن&shy; کوانتوم&shy; دات-منیزی</span>م </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">&shy;درمحدوده 40-36 نانومتر به&shy;دست آمد.</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">نتایج طیف </span></span></span>&nbsp;<span dir="LTR" style="color:black">FTIR</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">نشان&shy;دهنده</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">تشکیل پیوند </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بین </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">کربن&shy;کوانتوم&shy;دات-منیزیم</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> و اسانس زنیان </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">در</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">نانوکامپوزیت&shy;</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">می&shy;باشد.</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> اثر ضدمیکروبی نانوکامپوزیت&shy;</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">زنیان/</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">کیتوزان/کربوکسی&shy;متیل&shy;نشاسته/کربن&shy;کوانتوم&shy;دات-منیزیم</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> (168/0میلی&shy;گرم&shy;برمیلی&shy;لیتر) به&shy; دست&shy;آمد. </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج </span></span>&nbsp;RT-PCR<span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نشان داد، بیان ژن&shy;</span></span> <i>gtfC</i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">به&shy;طور معناداری</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">(049/0</span></span>P=<span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) در سلول&shy;های تیمار شده با نانوکامپوزیت کاهش یافته</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">، که نشان&shy;دهنده اثر قوی نانوکامپوزیت </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">زنیان/</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">کیتوزان/کربوکسی&shy;متیل&shy;نشاسته/کربن&shy;کوانتوم&shy;دات-منیزیم</span></span><b> </b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">در</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">سرکوب ژن &shy;</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">&shy;</span></span> <i>gtfC</i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">و </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">کاهش تشکیل بیوفیلم است. </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ارتباط معناداری در </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">بیان ژن&shy;های </span></span></span><i>gtfB</i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و</span></span></i><i>gtfD</i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، در سلول&shy;های تیمار شده با نانوکامپوزیت نسبت به گروه کنترل مشاهده نشد (067/0</span></span>, P=<span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">187/0</span></span>P=<span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">).</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="background:white"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">نتیجه&shy; گیری</span></span></b><b><span dir="LTR" style="font-size:14.0pt">:</span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">طبق نتایج </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">میزان بیان ژن</span></span> <i>gftC</i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">در سلول&shy;های تحت درمان با نانوکامپوزیت</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> حاوی زنیان<span style="color:black"> کاهش داشته و نانوکامپوزیت داری اثر قوی در </span>سرکوب ژن&shy;ها <span style="color:black">و </span>کاهش تشکیل بیوفیلم میکروبی دندان است.<b> </b></span></span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:8.0pt"></span></b></span></span></span></span></span> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt"><span style="line-height:107%">Aim and Background</span></span></b><span style="font-size:11.0pt"><span style="line-height:107%">: The bacterium <i>Streptococcus mutans</i> is the main cause of dental caries. The formation of biofilms of this bacterium depends on the presence of the enzyme glucosyltransferase, which is encoded by the <i>gtf</i> gene. The aim of this study was evaluated the effect of polysaccharide nanocomposites of Chitosan(Cs)/Carboxymethyl Starch(CMS) /</span></span><span style="font-size:11.0pt"><span style="line-height:107%">CQD.Mg</span></span><span style="font-size:11.0pt"><span style="line-height:107%"> /Trachyspermum on <i>gtf</i> gene expression in <i>Streptococcus mutans </i>using Real Time-PCR method in the formation of dental biofilm.</span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt"><span style="line-height:107%">Materials and Methods:</span></span></b><span style="font-size:11.0pt"><span style="line-height:107%"> After preparation of Chitosan(Cs)/Carboxymethyl Starch(CMS) /</span></span><span style="font-size:11.0pt"><span style="line-height:107%">CQD.Mg</span></span><span style="font-size:11.0pt"><span style="line-height:107%"> /Trachyspermum nanocomposite, its antimicrobial effect on <i>Streptococcus mutans</i> ATCC 35668 was investigated by microdilution method. The morphology and structural properties of the nanocomposite were investigated by FTIR and SEM</span></span> analysis<span style="font-size:11.0pt"><span style="line-height:107%">. The expression of <i>gtfD, gtfC, gtfB</i> genes was examined by Real Time-PCR technique. Data analysis was performed by SPSS₂₂ software and the means were compared based on ANOVA at the level of 0.05.</span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt"><span style="line-height:107%">Results:</span></span></b><span style="font-size:11.0pt"><span style="line-height:107%"> The particle size of Chitosan(Cs)/Carboxymethyl Starch(CMS)/</span></span><span style="font-size:11.0pt"><span style="line-height:107%">CQD.Mg</span></span><span style="font-size:11.0pt"><span style="line-height:107%">/Trachyspermum nanocomposite was obtained in the range of 36-40 nm. The results of FTIR spectrum indicate the formation of a bond between carbon-quantum materials-magnesium and Trachyspermum essential oil in the nanocomposite. The antimicrobial effect of Cs/ CMS/</span></span><span style="font-size:11.0pt"><span style="line-height:107%">CQD.Mg</span></span><span style="font-size:11.0pt"><span style="line-height:107%">/Trachyspermum (0.168 mg/ ml) was obtained. RT-PCR results showed that <i>gtfC</i> gene expression was significantly reduced (P=0.049) in nanocomposite-treated cells, indicating the strong effect of Cs/CMS/</span></span><span style="font-size:11.0pt"><span style="line-height:107%">CQD.Mg</span></span><span style="font-size:11.0pt"><span style="line-height:107%">/Trachyspermum nanocomposite suppresses <i>gtfC</i></span></span> <span style="font-size:11.0pt"><span style="line-height:107%">gene and reduces biofilm formation. No significant relationship was observed in the expression of <i>gtfB</i> and <i>gtfD</i> genes in nanocomposite-treated cells compared to the control group (P = 0.067, P = 0.187).</span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:11.0pt"><span style="line-height:107%">Conclusion:</span></span></b><span style="font-size:11.0pt"><span style="line-height:107%"> According to the results, the expression of <i>gftC</i> gene in cells treated with nanocomposite containing Trachyspermum has decreased and nanocomposite has a strong effect in suppressing genes and reducing the formation of microbial biofilm of teeth. </span></span></span></span></span></span><br> <span style="font-size:10pt"><span style="unicode-bidi:embed"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><span style="font-size:11.0pt"><span style="line-height:107%">.</span></span></span></span></span></span><br> &nbsp; ژن gtf,‌ استرپتوکوکوس‌موتانس, نانوکامپوزیت, بیوفیلم دندانی, Iau Science Gene expression, Streptococcus mutans, Polysaccharide, Dental biofilm, Iau Science 23 36 http://ncmbjpiau.ir/browse.php?a_code=A-10-1224-3&slc_lang=fa&sid=1 Shokofeh Mazhab jafari شکوفه مذهب جعفری shokofemj@yahoo.com 100319475328460013459 100319475328460013459 No Faculty of Bioscience, Tehran North Branch, Islamic Azad University, Tehran, Iran واحد تهران شمال، دانشگاه آزاد اسلامی Fatemeh Yazdian فاطمه یزدیان yazdian@ut.ac.ir 100319475328460013460 100319475328460013460 Yes Department of Life Science Engineering, Faculty of New Science and Technologies, University of Tehran, Tehran, Iran دانشگاه تهران Farzaneh Hosseini فرزانه حسینی Hosseinimicrobiology@gmail.com 100319475328460013461 100319475328460013461 No Faculty of Bioscience, Tehran North Branch, Islamic Azad University, Tehran, Iran دانشگاه آزاد اسلامی، واحد تهران شمال Behnam Rasekh بهنام راسخ rasekhb@ripi.ir 100319475328460013462 100319475328460013462 No Director Environment & Biotechnology Research Division, Research Institute of Petroleum Industry (RIPI), Tehran, Iran پژوهشگاه صنعت نفت ، تهران، ایران