fa تاثیرنانوذرات نقره سنتزشده ازعصاره هیدروالکلی گیاه انگشتانه (Digitalis nevrosa ) بر رده سلولی سرطان پستان MCF-7 فیزیولوژی Physiology مقاله پژوهشی Research Article <span style="font-size:11pt"><span style="line-height:115%"><span style="tab-stops:486.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">سابقه و هدف</span></span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">: یکی از روش&shy;های سنتز دوستدار محیط زیست سنتز نانوذرات با استفاده از گیاهان است. از نانوذرات فلزی دارای فعالیت ضدسرطانی، نانوذرات نقره می&shy;باشد. هدف از این مطالعه بررسی تاثیر نانوذارت <i>نقره </i>سنتز شده از عصاره &nbsp;هیدروالکلی گیاه گل&shy;انگشتانه(</span></span></span></span><i><span dir="LTR" style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Digitalis nervosa</span></span></span></i><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">) بر رده سلولی سرطان پستان </span></span></span></span><i><span dir="LTR" style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">&nbsp;MCF-7</span></span></span></i><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black"> می باشد.</span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:115%"><span style="tab-stops:486.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">مواد و روش&shy;ها</span></span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">:</span></span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">دراین مطالعه تجربی، نانوذرات نقره از عصاره هیدروالکلی گیاه &nbsp;گل&shy;انگشتانه سنتز شد. </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">سپس سلول&shy;های رده</span></span></span></span><span dir="LTR" style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> MCF-7 </span></span></span><span style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">&nbsp;با غلظت&shy;های متفاوت نانوذره نقره</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">(</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">125/3، 25/6، 5/12، 25، 50</span></span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">و</span></span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">100</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">میکرو</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">گرم بر میلی لیتر)</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black"> تیمار شدند. اثرات سمیت نانوذر</span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">ات </span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">نقره با روش</span></span></span></span><span dir="LTR" style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> MTT </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">و </span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">بعد از تعیین غلظت&shy;50درصد کشندگی نانودرات نقره،</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">تغییرات بیان ژن&shy;های</span></span></span></span> <i><span dir="LTR" style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">P53</span></span></span></i> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">، </span></span></span></span><i><span dir="LTR" style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Bax</span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">، </span></span></span></span></i><i><span dir="LTR" style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Bcl2</span></span></span></i><span style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black"> و </span></span></span></span><i><span dir="LTR" style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">CDH1</span></span></span></i> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">با روش</span></span></span></span><span dir="LTR" style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> Real-time PCR &nbsp;</span></span></span><span style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">&nbsp;بررسی شد. داده&shy;های حاصل با استفاده از نرم افزار</span></span></span></span><span dir="LTR" style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">SPSS</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black"> و آزمون آماری واریانس یک طرفه و تست توکی آنالیز شد. </span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:115%"><span style="tab-stops:486.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">یافته</span></span></span></span></b><b><i><span dir="LTR" style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">&shy;</span></span></span></i></b><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">ها</span></span></span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">: </span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">نتایج حاصل از </span></span></span></span><span dir="LTR" style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">MTT</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black"> نشان داد اثر نانوذرات نقره بر زنده&shy;مانی رده سلولی </span></span></span></span><span dir="LTR" style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">MCF-7</span></span></span><span style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black"> وابسته به دوز بوده و بیشترین خاصیت سیتوتوکسیسیتی مربوط به غلظت 100 و کمترین مربوط به25/6میکروگرم بر میلی&shy;لیتر به صورت معنادار می باشد </span></span></span></span><span dir="LTR" style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">.(P<0.001) </span></span></span><span style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">&nbsp;همچنین تاثیر نانو ذرات نقره با غلظت</span></span></span></span><span dir="LTR" style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">&shy;</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">50 درصد کشندگی( 55/19میکروگرم بر میلی&shy;لیتر) بر بیان ژن&shy;های</span></span></span></span> <i><span dir="LTR" style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">P53 </span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">، </span></span></span></span></i><i><span dir="LTR" style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Bax </span></span></span></i><i><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">،&nbsp; </span></span></span></span></i><i><span dir="LTR" style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Bcl2</span></span></span></i><i><span style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black"> و </span></span></span></span></i><i><span dir="LTR" style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">CDH1</span></span></span></i><span style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black"> در مقایسه با ژن کنترل تغییرات معناداری را نشان داد.</span></span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:115%"><span style="tab-stops:486.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">نتیجه گیری</span></span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">:</span></span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">نانو ذرات نقره سنتز شده به روش سبز از گیاه <i>گل انگشتانه</i> باعث القاء آپوپتوز در </span></span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">رده سلول</span></span></span></span> <span dir="LTR" style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">MCF-7</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">می</span></span></span></span><span dir="LTR" style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">&shy;</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">شود و استفاده از این نانو ذرات می</span></span></span></span><span dir="LTR" style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">&shy;</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black">تواند به عنوان یک انتخاب امیدوار کننده در درمان سرطان پستان مورد توجه قرار گیرد.</span></span></span></span></span></span></span></span></span></span><br> <br> &nbsp; <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times=""><span style="color:black">Background and aim:</span></span></b><span new="" roman="" style="font-family:" times=""><span style="color:black"> One of the environmentally friendly synthesis methods is the synthesis of nanoparticles using plants. Silver nanoparticles are metal nanoparticles with anticancer activity</span></span><span dir="RTL" lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">.</span></span></span><span new="" roman="" style="font-family:" times=""><span style="color:black"> This study aims&nbsp; to investigate the effect of silver nanoparticles synthesized from <i>Digitalis nervosa</i> hydroalcoholic extract on MCF-7 breast cancer cell line.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><span new="" roman="" style="font-family:" times=""><span style="color:black"></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:115%"><span style="tab-stops:486.0pt"><span calibri="" style="font-family:"><b><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Materials and Methods:</span></span></span></b><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black"> In this experimental study, silver nanoparticles were synthesized from the hydro alcoholic extract of the </span></span></span><i><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Digitalis nervosa</span></span></span></i><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">.MCF-7 cells were then treated with different concentrations of silver(3.125, 6.25, 12.5,25,50 and 100</span></span></span> <span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">&mu;g /ml).The toxicity effects of silver nanoparticles were analyzed by MTT method and after determining the concentration of 50% lethality of silver nanoparticles, the expression changes of P53,Bax, Bcl2 and CDH1 genes were investigated by Real - time PCR method. The obtained data were analyzed using SPSS software and one-way variance statistical test and Tukey&#39;s test.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:115%"><span style="tab-stops:486.0pt"><span calibri="" style="font-family:"><b><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Results: </span></span></span></b><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">The results of MTT showed that the effect of silver nanoparticles on the viability of MCF-7 cell line is dose-dependent and the highest cytotoxicity is related to the concentration of 100 and the lowest is related to 6.25 &mu;g /ml in a significant way. (P<0.001) Also, the effect of silver nanoparticles with a concentration of 50% lethality (19.55 micrograms/ml) on the expression of P<i>53, Bax, Bcl2 </i>and<i> CDH1</i> genes showed significant changes compared to the control gene.</span></span></span><span dir="RTL" lang="AR-SA" style="font-size:14.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:115%"><span style="tab-stops:486.0pt"><span calibri="" style="font-family:"><b><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Conclusion: </span></span></span></b><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Silver nanoparticles synthesized by green method from </span></span></span><i><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">Digitalis nervosa</span></span></span></i><span style="line-height:115%"><span new="" roman="" style="font-family:" times=""><span style="color:black">&nbsp; induce apoptosis in MCF-7 cell line and the use of these nanoparticles can be considered as a promising choice in the treatment of breast cancer. </span></span></span><span dir="RTL" lang="AR-SA" style="font-size:14.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span></span><br> <br> &nbsp; نانو­ذرات نقره, گل انگشتانه, زنده­مانی, آپوپتوز, سرطان پستان , ,Science Iau silver nanoparticles, Digitalis nervosa, viability, apoptosis, breast cancer, Science Iau 97 109 http://ncmbjpiau.ir/browse.php?a_code=A-10-1664-5&slc_lang=fa&sid=1 Fatemeh Javadi فاطمه جوادی 100319475328460014293 100319475328460014293 Yes Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran گروه زیست شناسی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران. Nastaran Asgharei Moghaadm نسترن اصغری مقدم 100319475328460014294 100319475328460014294 No Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran گروه زیست شناسی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران. Zahra Keshtmand زهرا کشتمند zkeshtmand2001@gmail.com 100319475328460014295 100319475328460014295 No Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran گروه زیست شناسی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران.