fa بررسی سلول‌های T تنظیم‌کننده (CD4+/FOXP3+) و میزان بیان ایندولامین 2 و3 دی اکسیژناز در لوسمی حاد لنفوئیدی فیزیولوژی Physiology مقاله پژوهشی Research Article <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">سابقه و هدف:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">با توجه به اینکه میزان آنزیم مهارکننده پاسخ‌های ایمنی به نام </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ایندول آمین 3،2-دی اکسیژناز (</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">IDO</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) و&nbsp; فعالیت</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> سلول‌های </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">Treg</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در برخی از بیماران سرطانی و همچنین لوسمی‌های حاد افزایش می‌یابد بطوری که این حالت می‌تواند منجر به مهار پاسخ‌های ایمنی گردد. </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">به </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ه</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مین منظور این مطالعه با هدف </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بررسی سلول‌های </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">T</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> تنظیم‌کننده </span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">(</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">CD4⁺/FOXP3⁺</span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">)</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و میزان بیان </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">IDO</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در لوسمی حاد</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">لنفوئیدی(</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">ALL</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) به روش فلوسایتومتری و </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">PCR</span> <span dir="LTR" new="" roman="" style="font-family:" times="">Time </span><span b="" nazanin="" style="font-family:">-</span><span dir="LTR" new="" roman="" style="font-family:" times="">Real </span>&nbsp;<span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">صورت گرفته‌است.</span></span> <b><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></b></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مواد و روش ها:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در این تحقیق از نمونه مغز استخوان 30 بیمار مبتلا به </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">ALL</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و 20 فرد سالم استفاده شد. سپس به کمک دو روش فلوسایتومتری و </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">Time PCR</span><span b="" nazanin="" style="font-family:">-</span><span dir="LTR" new="" roman="" style="font-family:" times="">Real</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> به ارزیابی میزان بیان </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">IDO</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و درصد سلول های </span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Treg</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> پرداخته شد. <b>یافته ها:</b> نتایج مطالعه حاضر نشان داد که درصد </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">Treg</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در لنفوسیت های کل، </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">T</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">Th</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در بیماران مبتلا به </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">ALL</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> به ترتیب به طور معناداری بیش از گروه نرمال بوده است (</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">P <0.001</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">). بعلاوه میزان بیان </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">IDO</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در گروه نرمال بطور معناداری کمتر از گروه </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">ALL</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> بوده‌است (</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">P =0.016</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">). </span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجه گیری:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">میزان بیان </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">IDO</span> <span b="" nazanin="" style="font-family:">درگروه</span><span dir="LTR" new="" roman="" style="font-family:" times="">ALL</span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">&nbsp;بطور معناداری بیشتر از افراد سالم بوده است. همچنین درگروه بیماران مبتلا به </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">ALL</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، ارتباط مثبت و غیرمعناداری میان میزان بیان </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">IDO</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> با درصدهای سلول</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های </span></span>&nbsp;<span dir="LTR" new="" roman="" style="font-family:" times="">Treg</span><span b="" nazanin="" style="font-family:">وجود</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> داشته است</span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">.</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> لذا بنظر می رسد انجام مطالعات بیشتر برای رفتار این لوسمی در بیان آنزیم </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">IDO</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و درصد سلول های </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">Treg</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و ارتباط آن ها با یکدیگر لازم و ضروری می باشد.</span></span><span dir="LTR" style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span><br> <br> &nbsp; <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></b></span></span></span><br> <span style="font-size:11pt"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Aim and Background:</span></span></span></b> <span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">The level of an immune response inhibitory enzyme, called indoleamine 2,3-dioxygenase (IDO), and the activity of Treg cells increase in some patients with cancer and acute leukemias such that it can lead to the inhibition of immune responses. Therefore, the very aim of the present study was to evaluate the regulatory T cells (CD4 + / FOXP3 +) and the level of the IDO expression in acute leukemias using flow cytometry and Real-Time PCR. </span></span></span>&nbsp;<b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Materials and Methods:</span></span></span></b> <span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">This study used bone marrow samples taken from&nbsp; 30 patients with acute lymphoid leukemia (ALL), and 20 healthy individuals. Then, the level of</span></span></span> <span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">the IDO expression and the percentage of Treg cells were evaluated using flow cytometry and Real-Time PCR. The results revealed that the percentage of Treg in the total lymphocytes, T and Th in the ALL group was significantly higher than that of the normal groups the respectively (P <0.001). </span></span></span><b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Results:</span></span></span></b> <span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">In addition, the level of the IDO expression</span></span></span> <span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">in the normal group was significantly lower than that of the ALL group (P= 0.016). </span></span></span><b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""> The level of the IDO expression</span></span></span> <span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">in the ALL groups was significantly higher than the normal group. Moreover, there was a positive and non-significant relationship between the level of the IDO expression</span></span></span> <span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">and the percentages of Treg cells in the ALL group. Therefore, it seems necessary to conduct more studies for the behavior of this leukemia in the expression of IDO enzyme and the percentage of Treg cells and their relationship with each other.</span></span></span><span style="font-size:12.0pt"><span style="line-height:107%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:107%"><span calibri="" style="font-family:"><span dir="RTL" lang="AR-SA" style="font-size:16.0pt"><span style="line-height:107%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span><br> &nbsp; ALL, فلوسایتومتری, Real -Time PCR Keywords: ALL, Flow cytometry, Real-Time PCR 45 57 http://ncmbjpiau.ir/browse.php?a_code=A-10-1845-2&slc_lang=fa&sid=1 Raziyeh Hakak راضیه حکاک 100319475328460015315 100319475328460015315 No Departman of Biology,Science Engineering Branch,Islamic Azad University Tehran north Branch,Tehran,Iran گروه زیست شناسی، دانشکده علوم زیستی،دانشگاه آزاد اسلامی واحد تهران شمال،تهران،ایران Behzad Poopak بهزاد پوپک bpoopak@gmail.com 100319475328460015316 100319475328460015316 Yes Islamic Azad University Tehran Medical Sciences, Tehran,Iran دانشگاه آزاد اسلامی، واحد علوم پزشکی،تهران،ایران Ahmad majd احمد مجد 100319475328460015317 100319475328460015317 No Departman of Biology, Islamic Azad University Tehran north Branch,Tehran,Iran گروه زیست شناسی، دانشگاه آزاد اسلامی واحد تهران شمال،تهران،ایران Monireh Movahedi منیره موحدی 100319475328460015318 100319475328460015318 No Departman of Biology, Islamic Azad University Tehran north Branch,Tehran,Iran گروه زیست شناسی، دانشگاه آزاد اسلامی واحد تهران شمال،تهران،ایران