New Cellularand Molecular Biotechnology Journal
مجله تازه هاي بيوتكنولوژي سلولي و مولكولي
NCMBJ
Basic Sciences
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تعیین دوز موثر کادمیوم بر میزان زیستایی و بیان ژن کانکسین 43 در رده سلولی RAW264-7
Determining the effective dose of cadmium on viability and connexin43 gene expression in RAW264-7 cell line
فیزیولوژی
Physiology
مقاله پژوهشی
Research Article
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">سابقه و هدف: </span></span></b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">فلزات سمی مانند کادمیوم و استرس اکسیداتیو ناشی از آن</span></span><span dir="LTR" style="font-family:"Times New Roman",serif">­</span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">ها می­تواند موجب پیشرفت بسیاری از بیماری­ها همچون آترواسکلروزیس شوند. آترواسکلروزیس یک پدیده­ی پیش رونده التهابی مزمن است که با تجمع چربی­ها در محل انشعاب عروق منجر به بروز بیماری­های ایسکمی دهنده می­شود. اختلال در عملکرد ژن­های بسیاری همچون کانکسین­ها که در هموستاز چربی­ها نقش دارند می­تواند موجب بروز آترواسکلروزیس شود. مطالعه حاضر به بررسی اثر کادمیوم بر میزان بیان ژن کانکسین 43 در رده سلولی </span></span><span dir="LTR" style="font-family:"Times New Roman",serif">RAW264-7</span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""> می­پردازد .</span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">مواد و روش ها: </span></span></b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">در این پژوهش<b> </b>رده سلولی </span></span><span dir="LTR" style="font-family:"Times New Roman",serif">RAW264-7</span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""> کشت داده و تحت تاثیر دوزهای مختلف کلرید کادمیوم قرار داده شد. </span></span><span dir="LTR" style="font-family:"Times New Roman",serif">IC50</span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""> با استفاده از تست </span></span><span dir="LTR" style="font-family:"Times New Roman",serif">MTT</span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""> تعیین، </span></span><span dir="LTR" style="font-family:"Times New Roman",serif">RNA</span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""> استخراج و میزان بیان ژن کانکسین 43 به روش </span></span><span dir="LTR" style="font-family:"Times New Roman",serif">Real-time PCR </span><span style="font-size:12.0pt"><span style="font-family:"B Nazanin""> ارزیابی شد. نتایج با استفاده از نرم­افزار آماری </span></span><span dir="LTR" style="font-family:"Times New Roman",serif"> SPSS</span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">با روش آماری </span></span><span dir="LTR" style="font-family:"Times New Roman",serif">one way ANOVA </span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""> آنالیز گردید. </span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">یافته ­ها: </span></span></b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">نتایج نشان می­دهد که بیان ژن کانکسین 43 در سلول­های آلوده شده توسط دوز 3/6 میکرومولار کادمیوم در زمان کوتاه نسبت به گروه کنترل افزایش معنی­داری نشان می­دهد. همچنین نتایج </span></span><span dir="LTR" style="font-family:"Times New Roman",serif">IC<sub>50</sub></span><span style="font-size:12.0pt"><span style="font-family:"B Nazanin""> بیانگر کاهش زیستایی سلول­های در این غلظت­ می­باشد.</span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="tab-stops:301.3pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">نتیجه­ گیری: </span></span></b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">به نظر می­رسد کادمیوم در غلظت­های متوسط و در زمان کوتاهی می­تواند با افزایش بیان ژن کانکسین 43 در رده سلولی</span></span><span dir="LTR" style="font-family:"Times New Roman",serif">RAW264-7 </span><span style="font-size:12.0pt"><span style="font-family:"B Nazanin""> اثرات سایتوتوکسیک بر گیرنده­های </span></span><span dir="LTR" style="font-family:"Times New Roman",serif">Scavenger</span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""> سلول­های ماکروفاژی گذاشته و منجر به پیشرفت آتروسکلروزیس شود.</span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="EN" style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif">Aim and Background:</span></span></b> <span style="font-family:"Times New Roman",serif">Toxic</span><span lang="EN" style="font-family:"Times New Roman",serif"> metals such as cadmium and the oxidative stress caused by them can develop many Disease such as atherosclerosis. Atherosclerosis is a progressive chronic inflammatory disease that leads to the coronary ischemic with the accumulation of fats at the branching point of the vessels. Dysfunction of many genes, such as connexin, which play a role in lipid homeostasis, can cause atherosclerosis. The present study investigates the effect of cadmium on the expression level of connexin 43 gene in RAW264-7 cell line. </span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><span lang="EN" style="font-family:"Times New Roman",serif"></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="EN" style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif">Material and methods:</span></span></b><span lang="EN" style="font-family:"Times New Roman",serif"> In this study, RAW264-7 cell line was cultured and affected by different doses of cadmium chloride. IC<sub>50</sub> was determined using MTT test, RNA was extracted and connexin 43 gene expression was evaluated by Real Time PCR method. The results were analyzed using SPSS software with </span><span style="font-family:"Times New Roman",serif">o</span><span lang="EN" style="font-family:"Times New Roman",serif">neway ANOVA statistical method.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"> </span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="EN" style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif">Result:</span></span></b><span lang="EN" style="font-family:"Times New Roman",serif"> Our findings showed that the expression of connexin 43 gene in polluted cells by 6.3 μM cadmium had a significant increase compared with control group, especially in a short time. Also, the IC<sub>50</sub> results indicate a decrease in cell viability in this concentration. </span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><span lang="EN" style="font-family:"Times New Roman",serif"></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="EN" style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif">Conclusion:</span></span></b><span lang="EN" style="font-family:"Times New Roman",serif"> It seems that cadmium in moderate concentrations and in a short time can exert cytotoxic effects on the scavenger receptors of macrophage cells by increasing the expression of connexin 43 gene in RAW264-7 cell line and lead to the development of atherosclerosis.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span><br>
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<span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><span lang="EN" style="font-family:"Times New Roman",serif"></span></span></span></span></span><br>
کادمیوم , کانکسین 43 , رده سلولیRAW264-7 , آتروسکلروزیس, Iau Science.
cadmium, connexin 43, cell line RAW264-7, atherosclerosis, Iau Science.
43
52
http://ncmbjpiau.ir/browse.php?a_code=A-10-121-1&slc_lang=fa&sid=1
Vahid
Naseh
وحید
ناصح
Vahid55vet@yahoo.com
100319475328460014576
100319475328460014576
Yes
Department of Biology, Parand Branch, Islamic Azad university, Tehran, Iran.
گروه زیست شناسی، واحد پرند، دانشگاه آزاد اسلامی. تهران. ایران
Masoud
Salehypour
مسعود
صالحی پور
100319475328460014577
100319475328460014577
No
Ameneh
Moghadas
آمنه
مقدسی
100319475328460014578
100319475328460014578
No
Sahar
Farzaneh
سحر
فرزانه
100319475328460014579
100319475328460014579
No