other بررسی اثرات سمیت سلولی نانوذرات اکسید تیتانیوم (TiO2) بر روی رده سلولی Caco-2 سرطان روده سلولی و مولکولی Cellular and molecular مقاله پژوهشی Research Article <p><span style="font-size:11pt"><span style="font-family:Calibri,sans-serif"><strong><span style="font-size:12.0pt">سابقه و هدف:</span></strong> <span style="font-size:12.0pt">استفاده از ترکیبات نانو به‌عنوان </span><span style="font-size:12.0pt">کاندید در</span><span style="font-size:12.0pt">اثربخشی علیه سلول‌های سرطانی مطرح‌شده است. هدف از این مطالعه بررسی اثرات سمیت سلولی نانو ذرات</span><span style="font-size:12.0pt"> اکسید تیتانیوم</span> <span dir="LTR" style="font-size:10.0pt">(TiO₂)</span><span style="font-size:12.0pt"> بر روی رده سلولی سرطان روده </span><span dir="LTR" style="font-size:10.0pt">)</span> <span dir="LTR" style="font-size:10.0pt">&nbsp;&nbsp;(Caco-2</span><span style="font-size:12.0pt">است. </span></span></span><br> <span style="font-size:11pt"><span style="font-family:Calibri,sans-serif"><strong><span style="font-size:12.0pt">مواد و روش‌ها:</span></strong><span style="font-size:12.0pt"> ویال حاوی &nbsp;سلول&nbsp; </span><span dir="LTR" style="font-size:10.0pt">Caco-2</span><span style="font-size:12.0pt"> &nbsp;در محیط کشت سلولی </span><span dir="LTR" style="font-size:10.0pt">RPMI 1640</span><span style="font-size:12.0pt">&nbsp; کشت داده شد. </span><span style="font-size:12.0pt">جهت ارزیابی اثرات سمی، نانو ذرات اکسید تیتانیوم &nbsp;در </span><span style="font-size:12.0pt">غلظت</span><span style="font-size:12.0pt">‌</span><span style="font-size:12.0pt">های 1 ، 5 ،10، 25 ،50 و 100 </span><span style="font-size:12.0pt">میکروگرم به ازای میلی‌لیتر </span><span style="font-size:12.0pt">در فواصل زمانی 24, 48 و 72 ساعت برروی رده سلولی سرطان روده </span><span style="font-size:12.0pt">بررسی و میزان سمیت به روش </span><span dir="LTR" style="font-size:10.0pt">MTT</span> <span style="font-size:12.0pt">اندازه‌گیری شد.</span></span></span><br> <span style="font-size:11pt"><span style="font-family:Calibri,sans-serif"><strong><span style="font-size:12.0pt">یافته&shy; ها</span></strong><strong><span style="font-size:12.0pt">:</span></strong><span style="font-size:12.0pt"> نتایج نشان داد که درصد زنده ماندن سلول‌های </span><span dir="LTR" style="font-size:10.0pt">Caco-2</span><span style="font-size:12.0pt"> در </span><span style="font-size:12.0pt">غلظت‌های مورد استفاده به ترتیب 14/96، </span><span style="font-size:12.0pt">41/90،&nbsp; ،44/69 </span><span style="font-size:12.0pt">، </span><span style="font-size:12.0pt">44/59</span><span style="font-size:12.0pt"> ، </span><span style="font-size:12.0pt">80/37</span><span style="font-size:12.0pt"> و51/33 </span>&nbsp;<span style="font-size:12.0pt">در صد می‌باشد</span><span style="font-size:12.0pt">. نانو ذرات اکسید تیتانیوم در غلظت&shy;های 1 و 5 میکروگرم بر میلی‌لیتر، اثر قابل‌توجهی بر </span><span style="font-size:12.0pt">تکثیر </span><span style="font-size:12.0pt">سلول&shy;های </span><span dir="LTR" style="font-size:10.0pt">Caco-2</span> <span style="font-size:12.0pt">ندارد. با افزایش غلظت نانوذرات تیتانیوم از 10 تا 100 میکروگرم بر میلی‌لیتر، اثرات سمیت سلولی &nbsp;قابل‌توجهی در سلول&shy;های </span><span dir="LTR" style="font-size:10.0pt">Caco-2</span><span style="font-size:12.0pt"> نسبت به سلول‌های کنترل (تیمارنشده) </span><span style="font-size:12.0pt">مشاهده شد</span><span style="font-size:12.0pt">. میزان </span><span dir="LTR" style="font-size:10.0pt">IC₅₀</span><span style="font-size:12.0pt">50 میکروگرم به ازای میلی تر بدست آمد. </span></span></span><br> <span style="font-size:11pt"><span style="font-family:Calibri,sans-serif"><strong><span style="font-size:12.0pt">نتیجه‌گیری: </span></strong><span style="font-size:12.0pt">مطالعه حاضر نشان می‌دهد که نانوذرات </span><span dir="LTR" style="font-size:10.0pt">TiO₂</span>_&nbsp;<span style="font-size:12.0pt">می‌توانند اثر سمی بر روی سلول‌های </span><span dir="LTR" style="font-size:10.0pt">Caco-2</span><span style="font-size:12.0pt"> داشته باشند و در توسعه داروهای ضد سرطان موثر باشند. </span></span></span><br> <br> &nbsp;</p> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Aim and Background:</span></span></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> The use of nano materials has been proposed as a candidate for cancer cells treatments. The aim of this study was to investigate the cytotoxic effects of titanium oxide nanoparticles (TiO₂) on colon cancer cell line (Caco-2).</span></span><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Material and Methods:</span></span></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> For the cell culture, the vial containing Caco-2 cells was cultured in RPMI 1640 medium. In order to evaluate the cytotoxic effects, titanium dioxide nanoparticles, in concentrations of 1, 5, 10, 25, 50 and 100 micrograms per milliliter were used on Caco-2 cells at time intervals of 24, 48 and 72 hours. Toxicity was measured by MTT method.&nbsp; </span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Results:</span></span></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> The results showed that the survival percentage of Caco-2 cells in the used concentrations of TiO₂ were 96</span></span><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">.</span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">14%, 90</span></span><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">.</span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">41%, 69</span></span><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">.</span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">44%, 59</span></span><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">.</span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">44%, 37</span></span><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">.</span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">80, and 31</span></span><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">.</span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">51%, respectively. Based on the obtained results, it was determined that titanium oxide nanoparticles in concentrations of 1 and 5 micrograms/ml have no significant effect on Caco-2 cells. However, by increasing the concentration of titanium nanoparticles from 10</span></span> <span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">&mu;g/mL to 100 &mu;g/mL, significant cytotoxic effects were observed in Caco-2 cells. Increasing titanium nanoparticle concentration from 10 to 100 &mu;g/mL showed significant cytotoxic effects in Caco-2 cells compared to control (untreated) cells. The IC₅₀ value was obtained at the concentration (50 &mu;g/mL).</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Conclusion: </span></span></b><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Overall, the present study shows that TiO₂ can have a toxic effect on Caco-2 cells and be effective in development of anticancer drugs.</span></span></span></span></span></span></span><br> &nbsp; نانو ذرات اکسید تیتانیوم, رده سلول Caco-2, اثرات سمیت سلولی, درصد زنده ماندن.  Titanium oxide Nanoparticles, Caco-2 cell line, Cytotoxic effects, Survival percentage. ​​​​​​​ 71 78 http://ncmbjpiau.ir/browse.php?a_code=A-10-1504-3&slc_lang=other&sid=1 Mohammad Faezi Ghasemi محمد فائزی قاسمی faezi_m@yahoo.com 100319475328460015158 100319475328460015158 Yes Department of Microbiology, Lahijan Branch, Islamic Azad University, Lahijan, Iran گروه میکروبیولوژی، واحد لاهیجان، دانشگاه آزاد اسلامی، لاهیجان، ایران Ali Deshmir علی دشمیر 100319475328460015159 100319475328460015159 No Department of Microbiology, Lahijan Branch, Islamic Azad University, Lahijan, Iran گروه میکروبیولوژی، واحد لاهیجان، دانشگاه آزاد اسلامی، لاهیجان، ایران