New Cellularand Molecular Biotechnology Journal
مجله تازه هاي بيوتكنولوژي سلولي و مولكولي
NCMBJ
Basic Sciences
http://ncmbjpiau.ir
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مطالعه مولکولی ژنهای مقاومت آنتیبیوتیکی در سویه های اشریشیاکلی جدا شده از لاشه های طیور گوشتی درکرمان به روش PCR چندگانه
Molecular study of antibiotic resistance genes in Escherichia coli strains from broiler chickens in Kerman by multiplex PCR technique
سلولی و مولکولی
Cellular and molecular
مقاله پژوهشی
Research Article
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="color:black">سابقه و هدف:</span></span></span></b> <i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">اشریشیاکُلی</span></span></i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">، بیماری</span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">زای پرندگان</span></span> <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">مسئول ایجاد طیف وسیعی از بیماری</span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">های خارج روده</span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">ای طیور مانند <i>کلی</i></span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">باسیلوز، سلولیت، کلی</span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">گرانولوما</span></span></i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""> و عفونت کیسۀ زرده می</span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">باشد. هدف از این مطالعه بررسی مولکولی ژن</span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">های مقاومت آنتیبیوتیکی از قبیل </span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">aac</span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">، </span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">aad</span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">، </span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">qnr</span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">، </span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">tet</span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">، </span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">sul</span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">، </span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">bla</span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""> و</span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">cat </span></span></i><span style="font-size:12.0pt"><span style="font-family:"B Nazanin""> به</span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">روش </span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">PCR</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""> چندگانه<i> </i>و تعیین حساسیت آنتیبیوتیکی میباشد.<span style="color:black"></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="color:#262626">مواد و روش</span></span></span></b><b><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"><span style="color:#262626"></span></span></span></b><b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="color:#262626">ها:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""> نمونه</span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">برداری از کُشتارگاه صنعتی کرمان طی 8 ماه در سال1400 انجام و تعداد 112 جدایه از نمونه</span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">های مبتلا به کلی</span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">باسیلوز و 56 نمونۀ سلولیتی جدا گردید و پس از تأیید بیوشیمیایی، آزمون</span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">های </span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"> PCR</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">چندگانه</span></span> <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">و تست حساسیت آنتی</span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">بیوتیکی روی نمونه</span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">ها صورت گرفت. </span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="color:#262626">یافته ­ها:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""> نتایج نشان داد که 85/65 درصد نمونه</span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">ها برای ژن </span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">tetA</span></span></i><i> </i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">و 65/64 درصد برای </span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">tetB</span></span></i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""> مثبت بودند. درصد فراوانی ژن</span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">های </span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">qnr<sub>S</sub></span></span></i><i> </i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">(3/47)، </span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">qnr<sub>B</sub></span></span></i> <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span><span style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span><m:omath><m:ssub><m:ssubpr><span style="font-size:10.0pt"><span style="font-family:"Cambria Math",serif"><span style="color:#171717"><span style="font-style:italic"><m:ctrlpr></m:ctrlpr></span></span></span></span></m:ssubpr><m:e><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Cambria Math",serif"><m:r><m:rpr><m:scr m:val="roman"><m:sty m:val="p"></m:sty></m:scr></m:rpr></m:r></span></span></m:e><m:sub><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Cambria Math",serif"><m:r><m:rpr><m:scr m:val="roman"><m:sty m:val="p"></m:sty></m:scr></m:rpr></m:r></span></span></m:sub></m:ssub></m:omath><span style="font-size:11.0pt"><span style="line-height:107%"><span style="font-family:"Calibri",sans-serif"><span style="position:relative"><span style="top:3.0pt"> </span></span></span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">(09/24)، </span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">sul<sub>I</sub></span></span></i> <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span><span style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span><m:omath><m:ssub><m:ssubpr><span style="font-size:10.0pt"><span style="font-family:"Cambria Math",serif"><span style="color:#171717"><span style="font-style:italic"><m:ctrlpr></m:ctrlpr></span></span></span></span></m:ssubpr><m:e><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Cambria Math",serif"><m:r><m:rpr><m:scr m:val="roman"><m:sty m:val="p"></m:sty></m:scr></m:rpr></m:r></span></span></m:e><m:sub><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Cambria Math",serif"><m:r><m:rpr><m:scr m:val="roman"><m:sty m:val="p"></m:sty></m:scr></m:rpr></m:r></span></span></m:sub></m:ssub></m:omath><span style="font-size:11.0pt"><span style="line-height:107%"><span style="font-family:"Calibri",sans-serif"><span style="position:relative"><span style="top:3.0pt"> </span></span></span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">(91/28)، </span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">dhfr<sub>V</sub></span></span></i> <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span><span style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span><m:omath><m:ssub><m:ssubpr><span style="font-size:10.0pt"><span style="font-family:"Cambria Math",serif"><span style="color:#171717"><span style="font-style:italic"><m:ctrlpr></m:ctrlpr></span></span></span></span></m:ssubpr><m:e><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Cambria Math",serif"><m:r><m:rpr><m:scr m:val="roman"><m:sty m:val="p"></m:sty></m:scr></m:rpr></m:r></span></span></m:e><m:sub><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Cambria Math",serif"><m:r><m:rpr><m:scr m:val="roman"><m:sty m:val="p"></m:sty></m:scr></m:rpr></m:r></span></span></m:sub></m:ssub></m:omath><span style="font-size:11.0pt"><span style="line-height:107%"><span style="font-family:"Calibri",sans-serif"><span style="position:relative"><span style="top:3.0pt"> </span></span></span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">(32/41)،</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span><span style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span><m:omath><m:ssub><m:ssubpr><span style="font-size:10.0pt"><span style="font-family:"Cambria Math",serif"><span style="color:#171717"><span style="font-style:italic"><m:ctrlpr></m:ctrlpr></span></span></span></span></m:ssubpr><m:e><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Cambria Math",serif"><m:r><m:rpr><m:scr m:val="roman"><m:sty m:val="p"></m:sty></m:scr></m:rpr></m:r></span></span></m:e><m:sub><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Cambria Math",serif"><m:r><m:rpr><m:scr m:val="roman"><m:sty m:val="p"></m:sty></m:scr></m:rpr></m:r></span></span></m:sub></m:ssub></m:omath><span style="font-size:11.0pt"><span style="line-height:107%"><span style="font-family:"Calibri",sans-serif"><span style="position:relative"><span style="top:3.0pt"> </span></span></span></span></span> <i><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">dhfr<sub>A</sub></span></span></i><i> </i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">(5/26)، 1-(3) </span></span><i> </i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">aac</span></span></i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">(66/25)، </span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">aad<sub>A</sub></span></span></i><i> </i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span><span style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span><m:omath><m:ssub><m:ssubpr><span style="font-size:10.0pt"><span style="font-family:"Cambria Math",serif"><span style="color:#171717"><span style="font-style:italic"><m:ctrlpr></m:ctrlpr></span></span></span></span></m:ssubpr><m:e><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Cambria Math",serif"><m:r><m:rpr><m:scr m:val="roman"><m:sty m:val="p"></m:sty></m:scr></m:rpr></m:r></span></span></m:e><m:sub><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Cambria Math",serif"><m:r><m:rpr><m:scr m:val="roman"><m:sty m:val="p"></m:sty></m:scr></m:rpr></m:r></span></span></m:sub></m:ssub></m:omath><span style="font-size:11.0pt"><span style="line-height:107%"><span style="font-family:"Calibri",sans-serif"><span style="position:relative"><span style="top:3.0pt"> </span></span></span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">(89/32)، </span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">flor</span></span></i><span style="font-size:12.0pt"><span style="font-family:"B Nazanin""> (95/28)، </span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">sul<sub>II</sub></span></span></i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""> (31/43)</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""> و </span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">qnr<sub>A</sub></span></span></i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span><span style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span><m:omath><m:ssub><m:ssubpr><span style="font-size:10.0pt"><span style="font-family:"Cambria Math",serif"><span style="color:#171717"><span style="font-style:italic"><m:ctrlpr></m:ctrlpr></span></span></span></span></m:ssubpr><m:e><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Cambria Math",serif"><m:r><m:rpr><m:scr m:val="roman"><m:sty m:val="p"></m:sty></m:scr></m:rpr></m:r></span></span></m:e><m:sub><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Cambria Math",serif"><m:r><m:rpr><m:scr m:val="roman"><m:sty m:val="p"></m:sty></m:scr></m:rpr></m:r></span></span></m:sub></m:ssub></m:omath><span style="font-size:11.0pt"><span style="line-height:107%"><span style="font-family:"Calibri",sans-serif"><span style="position:relative"><span style="top:3.0pt"> </span></span></span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""> (30/22) درصد بود. هیچ</span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">کدام از نمونه</span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span></i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">ها از نظر ژن</span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">های </span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">bla<sub>SHV</sub></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">، </span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">bla<sub>CTX</sub></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">، </span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">bla<sub>TEM</sub></span></span></i><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">، </span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">bal<sub>OXA</sub></span></span></i><i> </i><i> </i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span><span style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span><m:omath><m:ssub><m:ssubpr><span style="font-size:10.0pt"><span style="font-family:"Cambria Math",serif"><span style="color:#171717"><span style="font-style:italic"><m:ctrlpr></m:ctrlpr></span></span></span></span></m:ssubpr><m:e><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Cambria Math",serif"><m:r><m:rpr><m:scr m:val="roman"><m:sty m:val="p"></m:sty></m:scr></m:rpr></m:r></span></span></m:e><m:sub><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Cambria Math",serif"><m:r><m:rpr><m:scr m:val="roman"><m:sty m:val="p"></m:sty></m:scr></m:rpr></m:r></span></span></m:sub></m:ssub></m:omath><span style="font-size:11.0pt"><span style="line-height:107%"><span style="font-family:"Calibri",sans-serif"><span style="position:relative"><span style="top:3.0pt"> </span></span></span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span><span style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span><m:omath><m:ssub><m:ssubpr><span style="font-size:10.0pt"><span style="font-family:"Cambria Math",serif"><span style="color:#171717"><span style="font-style:italic"><m:ctrlpr></m:ctrlpr></span></span></span></span></m:ssubpr><m:e><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Cambria Math",serif"><m:r><m:rpr><m:scr m:val="roman"><m:sty m:val="p"></m:sty></m:scr></m:rpr></m:r></span></span></m:e><m:sub><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Cambria Math",serif"><m:r><m:rpr><m:scr m:val="roman"><m:sty m:val="p"></m:sty></m:scr></m:rpr></m:r></span></span></m:sub></m:ssub></m:omath><span style="font-size:11.0pt"><span style="line-height:107%"><span style="font-family:"Calibri",sans-serif"><span style="position:relative"><span style="top:3.0pt"> </span></span></span></span></span> <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">مثبت نبودند. همچنین 50 تا 85 درصد جدایهها به یک یا چند آنتیبیوتیک مقاوم بودند. بیشترین درصد مقاومت به آنتی</span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">بیوتیک</span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">های تتراسایکلین (18/94)، سولفامتوکسازول (24/61)،</span></span> <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">کلرامفنیکل (96/45) و تریمتوپریم (36/46) درصد بود.</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="color:#262626"></span></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""><span style="color:#262626">نتیجه­ گیری: </span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">در این مطالعه مشخص گردید که جهت درمان قطعی و جلوگیری از بروز مقاومت</span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">های روزافزون در سویه</span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">های پاتوژن، تعیین الگوی مقاومتی میکروارگانیسم و انجام تست حساسیت آنتی</span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">بیوتیکی قبل از تجویز دارو نقش مؤثری دارد. </span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-family:"Times New Roman",serif">Aim and Background:</span></b><span style="font-size:10.0pt"><span style="background:white"><span style="font-family:"Times New Roman",serif"><span style="color:black"> Avian pathogenic <i>E. coli</i> is responsible for an extremely wide range of extraintestinal diseases in poultry, such as</span></span></span></span> <i><span style="font-size:10.0pt"><span style="background:white"><span style="font-family:"Times New Roman",serif"><span style="color:black">colibacillosis, cellulitis, coligranuloma</span></span></span></span></i><span style="font-size:10.0pt"><span style="background:white"><span style="font-family:"Times New Roman",serif"><span style="color:black">, and yolk sac infection. The aim of this study is the molecular study of antibiotic-resistant resistance genes, such as <i>bla</i>, <i>aac</i>, <i>aad</i>, <i>qnr</i>, <i>sul</i>, <i>tet,</i> and <i>cat</i>, by the multiplex PCR method and the determination of antibiotic susceptibility.</span></span></span></span><span style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-family:"Times New Roman",serif">Materials and Methods:</span></b> <span style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">One hundred and twelve strains of <i>E. coli</i> from colibacillosis cases and fifty-six strains from <i>cellulitis</i> cases were isolated from poultry slaughterhouses in Kerman province during eight months in 2021. The strains of <i>E. coli</i> were confirmed by biochemical methods, and then the PCR experiment and antibiotic susceptibility test were performed on the samples.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-family:"Times New Roman",serif">Results</span></b><b><span style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">:</span></span></b><span style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"> The results of the studies showed that 65.85% of the samples for the <i>tetA</i> gene and 64.65% of those for the <i>tetB</i> gene were positive. Percentage of genes <i>qnrS</i> (47.3), <i>qnrB</i> (24.09), <i>sulI</i> (28.91), <i>dhfrV</i> (41.32), <i>dhfrA</i> (26.5), <i>aac (3)-1</i> (25.66), <i>aadA</i> (32.89), <i>floR</i> (28.95), <i>sulII</i> (43.31),</span></span> <span style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">and <i>qnrA</i> (22.30) %. None of the samples were positive with regard to <i>blaoxa</i>, <i>blaCTX</i>, <i>blasHV</i> and <i>blaTEM</i> genes. In this study, it was specified that 50-85 percent of isolates of all strains were resistant to one or more antibiotics. The highest percentage of resistance to antibiotics was, respectively, to tetracycline (94.18%), sulfamethoxazole (61.24), chloramphenicol (45.96), and trimethoprim (46.36) %.</span></span><span style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-family:"Times New Roman",serif">Conclusion:</span></b> <span style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">In this study. Recognition of resistance patterns and microorganisms to susceptibility to antibiotics plays an effective role in the correct and suitable selection of antibiotics and infection control.</span></span><span style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span><br>
اشریشیاکُلی, سلولیت, کلیباسیلوز, جوجههای گوشتی, مقاومت آنتیبیوتیکی.
Escherichia coli, celluitis, collibacillosis, Broiler chicken, Antibiotic resistance.
39
48
http://ncmbjpiau.ir/browse.php?a_code=A-10-1438-6&slc_lang=other&sid=1
Akbar
Asadi
اکبر
اسدی
dr.asadi44@yahoo.com
100319475328460015329
100319475328460015329
Yes
Islamic Azad University, Shahr Babak Branch
دانشگاه آزاد اسلامی واحد شهربابک
Navid
Asadi
نوید
اسدی
100319475328460015330
100319475328460015330
No
Shahid Bahonar University of Kerman
دانشگاه شهید باهنر کرمان