other بررسی اثرات ضد باکتریایی وآنتی اکسیدانی نانوذرات اکسید روی سنتز شده از Arthrospira platensis با هدف ترمیم زخم سلولی و مولکولی Cellular and molecular مقاله پژوهشی Research Article <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">سابقه و هدف:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> مراقبت از زخم</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ها بار مالی قابل</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">توجهی بر نظام سلامت جامعه تحمیل می</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نماید. از مهم</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ترین عوامل کلیدی و مؤثر تأخیر در بهبود زخم، عفونت زخم و سطوح بالای گونه</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های اکسیژن فعال (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">ROS</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) می</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">باشد. مطالعۀ حاضر به بررسی سنتز سبز نانوذرات اکسید روی (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">ZnO</span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">NPs</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) با استفاده از عصارۀ آبی <i>آرتروسپیرا</i> <i>پلاتنسیس</i> (</span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Arthrospira</span></span></i><i> </i><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">platensis</span></span></i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) می</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">پردازد.</span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مواد و روش</span></span></b><b><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span></b><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ها:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> سنتز نانوذرات </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">ZnO</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> با طیف</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">سنجی </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">UV-Vis</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">FTIR</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> موردبررسی قرار گرفت و درنهایت برای فعالیت ضدباکتریایی به</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">روش انتشار در چاهک آگار و فعالیت آنتی</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">اکسیدانی با سنجش </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">DPPH</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> آزمایش گردید. </span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">یافته&shy; ها:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> نتایج سنتز بیولوژیکی با استفاده از عصارۀ آبی <i>آرتروسپیرا پلاتنسیس</i>، شکل</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">گیری یکنواخت و تشکیل گروه</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های عاملی مؤثر بر نانوذرات </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">ZnO</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و اندازۀ متوسط نانوذرات تشکیل</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">شده و پایداری ذرات معلّق را توسط طیف</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">FTIR</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">, </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">UV-VIS</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">, </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">DLS</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">, </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">ZETA Potential</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">&nbsp; تأیید کرد. نانوذرات سنتز شده، فعالیت ضدباکتریاییِ خوبی در برابر باکتری</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">های مؤثر در عفونت زخم مانند</span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Escherichia coli</span></span></i><i> </i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">(</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">PTCC1399</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">)</span></span><b>&nbsp;</b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Pseudomonas aeruginosa</span></span></i><i> </i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">(</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">PTCC1430</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) را از خود نشان دادند. حداکثر قطر هالۀ عدم</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">رشد باکتری </span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">E</span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">. </span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">coli</span></span></i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> برابر با 32 میلی</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">متر در غلظت 40 میلی</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">گرم بر میلی</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">لیتر و در غلظت مشابه برای باکتری </span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P</span></span></i><i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">. </span></span></i><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">aeruginosa</span></span></i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> برابر با 34 میلی</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">متر برآورد شد. همچنین فعالیت آنتی</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">اکسیدانی نانوذرات سنتز شده، بیشترین مهار رادیکال آزاد </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">DPPH</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> با میزان 60/66 درصد در غلظت 25/9 میلی</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">گرم بر میلی</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">لیتر را نشان داد.</span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجه</span></span></b><b><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span></b><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">گیری:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> استفاده از چنین روش بیولوژیکیِ ساده و مقرون</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">به</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">صرفه</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ای می</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تواند الگوی جایگزینِ مناسبی برای سنتز نانومواد در مقیاس بزرگ باشد، همچنین از نانوذرات سنتز شده به این روش می</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">توان در تسریع روند بهبود زخم استفاده نمود.</span></span></span></span></span></span></span></span><br> &nbsp; <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="font-family:Calibri,sans-serif"><b><span lang="EN" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Aim and Background:</span></span></b><span lang="EN" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> Wound care imposes a significant financial burden on the health system<b>. </b>One of the most important and effective factors in delaying wound healing is wound infection and high levels of reactive oxygen species (ROS). The present study examines the green synthesis of zinc oxide nanoparticles (ZnO NPs) using the aqueous extract of Arthrospira platensis. </span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:12.95pt"><span style="text-autospace:none"><span style="font-family:Calibri,sans-serif"><b><span lang="EN" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Materials and Methods:</span></span></b><span lang="EN" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> The synthesis of ZnO nanoparticles was investigated by UV-Vis and FTIR spectroscopy and finally tested for antibacterial activity by diffusion method in agar well and antioxidant activity by DPPH assay.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:12.95pt"><span style="text-autospace:none"><span style="font-family:Calibri,sans-serif"><b><span lang="EN" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Results:</span></span></b><span lang="EN" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> The results of biological synthesis using the aqueous extract of Arthrospira platensis confirmed the uniform formation. Also, the formation of effective functional groups on ZnO nanoparticles, the average size of the formed nanoparticles and the stability of suspended particles were assessed by FTIR, UV-VIS, DLS, and ZETA Potential spectra. Synthesized nanoparticles showed good antibacterial activity against bacteria involved in wound infection, such as <i>E. coli</i> (PTCC1399) and Pseudomonas aeruginosa (PTCC1430). The maximum diameter of the halo of non-growth of <i>E. coli</i> and <i>P. aeruginosa</i> bacteria was estimated to be 32 and 34 mm, respectively, at a concentration of 40 mg/mL. Also, the antioxidant activity of the synthesized nanoparticles showed the highest DPPH free radical inhibition with a rate of 66.60 % at a concentration of 9.25 mg/mL. </span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="font-family:Calibri,sans-serif"><b><span lang="EN" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></b><span lang="EN" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> Using such a simple and cost-effective biological method can be a suitable alternative model for synthesizing nanomaterials on a large scale, accelerating the wound-healing process.</span></span></span></span></span></span><br> &nbsp; نانوذرات اکسید روی, آرتروسپیرا پلاتنسیس, اثر آنتی‌اکسیدانی, اثر ضد‌باکتریایی. Zinc oxide, green synthesis, antioxidant, antibacterial, wound healing. 49 58 http://ncmbjpiau.ir/browse.php?a_code=A-10-2010-2&slc_lang=other&sid=1 shabnam Akbari شبنم اکبری 100319475328460015343 100319475328460015343 No Department of Cognitive Science, Science and Research Branch, Islamic Azad University, Tehran, Iran گروه علوم‌شناختی˓ واحد علوم و تحقیقات˓ دانشگاه آزاد اسلامی، تهران، ایران Shiva Khalil-Moghadam شیوا خلیل مقدم shiva.moghaddam@yahoo.com 100319475328460015344 100319475328460015344 Yes Department of Biology, Faculty of Basic Sciences, Yadegar-e-Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran گروه زیست‌شناسی، واحد یادگار امام خمینی (ره) شهر ری، دانشگاه آزاد اسلامی، تهران، ایران Maryam Ghobeh مریم قبه 100319475328460015345 100319475328460015345 No Department of Cognitive Science, Science and Research Branch, Islamic Azad University, Tehran, Iran گروه علوم‌شناختی˓ واحد علوم و تحقیقات˓ دانشگاه آزاد اسلامی، تهران، ایران Maryam Bikhof Torbati مریم بی خوف تربتی 100319475328460015346 100319475328460015346 No Department of Biology, Yadegar-e-Imam Khomeini (RAH) Shahr-e- Rey Branch, Islamic Azad University, Tehran, Iran گروه زیست‌شناسی، واحد یادگار امام خمینی (ره) شهر ری، دانشگاه آزاد اسلامی، تهران، ایران Ashraf Sadat Shah Velayati اشرف سادات شاه ولایتی 100319475328460015347 100319475328460015347 No Department of Chemistry, Faculty of Basic Sciences, Yadegar-e-Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran گروه شیمی، دانشکده علوم پایه، واحد یادگار امام خمینی(ره) شهرری، دانشگاه آزاد اسلامی، تهران، ایران