other ارتباط بین حضور پلی مرفیسم rs34173062ژن شارپین و بیماری آلزایمر با روش Tetra ARMS PCR سلولی و مولکولی Cellular and molecular مقاله پژوهشی Research Article <span style="font-size:12pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span b="" lang="FA" nazanin="" style="font-family:">سابقه و هدف</span></b><span b="" lang="FA" nazanin="" style="font-family:">: مکانیسم</span><span dir="LTR" style="font-size:10.0pt"><span calibri="" style="font-family:">‌</span></span><span b="" lang="FA" nazanin="" style="font-family:">های مولکولی ایجادکنندۀ بیماری آلزایمر هنوز ناشناخته هستند. عوامل ژنتیکی و محیطیِ مختلف می</span><span dir="LTR" style="font-size:10.0pt"><span calibri="" style="font-family:">‌</span></span><span b="" lang="FA" nazanin="" style="font-family:">توانند در ایجاد بیماری آلزایمر مؤثر باشند و برای تشخیص و پیش</span><span dir="LTR" style="font-size:10.0pt"><span calibri="" style="font-family:">‌</span></span><span b="" lang="FA" nazanin="" style="font-family:">آگهی از این بیماری استفاده شوند. هدف از این مطالعه بررسی حضور پلی</span><span dir="LTR" style="font-size:10.0pt"><span calibri="" style="font-family:">‌</span></span><span b="" lang="FA" nazanin="" style="font-family:">مورفیسم</span><span dir="LTR" style="font-size:10.0pt">rs34173062 </span><span b="" nazanin="" style="font-family:">&nbsp;در ژن <i>شارپین</i> و ارتباط آن با ایجاد آلزایمر در جمعیتی از بیماران ایرانی بود. </span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span b="" lang="FA" nazanin="" style="font-family:">مواد و روش</span></b><b><span dir="LTR" style="font-size:10.0pt"><span calibri="" style="font-family:">‌</span></span></b><b><span b="" lang="FA" nazanin="" style="font-family:">ها</span></b><span b="" lang="FA" nazanin="" style="font-family:">: این مطالعه در سال 1400 به‌صورت مورد-شاهدی بر روی 50 فرد مبتلا به بیماری آلزایمر و 50 فرد سالم انجام گرفت. پس از استخراج </span><span dir="LTR" style="font-size:10.0pt">DNA </span><span b="" lang="FA" nazanin="" style="font-family:">&nbsp;از نمونه</span><span dir="LTR" style="font-size:10.0pt"><span calibri="" style="font-family:">‌</span></span><span b="" lang="FA" nazanin="" style="font-family:">های خون با روش </span><span dir="LTR" style="font-size:10.0pt">Tetra-ARMS PCR</span><span b="" lang="FA" nazanin="" style="font-family:"> ژنوتایپینگ انجام گردید و داده</span><span dir="LTR" style="font-size:10.0pt"><span calibri="" style="font-family:">‌</span></span><span b="" lang="FA" nazanin="" style="font-family:">ها آنالیز آماری شدند.</span></span></span></span></span></span><br> <span style="font-size:12pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span b="" lang="FA" nazanin="" style="font-family:">یافته&shy; ها:</span></b><span b="" lang="FA" nazanin="" style="font-family:"> نتایج ژنوتایپینگ برای پلی</span><span dir="LTR" style="font-size:10.0pt"><span calibri="" style="font-family:">‌</span></span><span b="" lang="FA" nazanin="" style="font-family:">مورفیسم</span><span dir="LTR" style="font-size:10.0pt">rs34173062 </span><span b="" lang="FA" nazanin="" style="font-family:">&nbsp;نشان داد که فراوانی ژنوتایپ</span><span dir="LTR" style="font-size:10.0pt"><span calibri="" style="font-family:">‌</span></span><span b="" lang="FA" nazanin="" style="font-family:">های</span><span dir="LTR" style="font-size:10.0pt">AA </span><span b="" lang="FA" nazanin="" style="font-family:">، </span><span dir="LTR" style="font-size:10.0pt">AG</span><span b="" lang="FA" nazanin="" style="font-family:"> و </span><span dir="LTR" style="font-size:10.0pt">GG</span><span b="" lang="FA" nazanin="" style="font-family:"> در گروه بیماران به</span><span dir="LTR" style="font-size:10.0pt"><span calibri="" style="font-family:">‌</span></span><span b="" lang="FA" nazanin="" style="font-family:">ترتیب 62/0، 10/0 و&nbsp; 28/0 و در گروه کنترل به</span><span dir="LTR" style="font-size:10.0pt"><span calibri="" style="font-family:">‌</span></span><span b="" lang="FA" nazanin="" style="font-family:">ترتیب 48/0، 18/0 و 34/0 بود. بین پلی</span><span dir="LTR" style="font-size:10.0pt"><span calibri="" style="font-family:">‌</span></span><span b="" lang="FA" nazanin="" style="font-family:">مورفیسم</span><span dir="LTR" style="font-size:10.0pt">rs34173062 </span><span b="" lang="FA" nazanin="" style="font-family:">&nbsp;&nbsp;و احتمال ابتلا به بیماری آلزایمر ارتباط معنی</span><span dir="LTR" style="font-size:10.0pt"><span calibri="" style="font-family:">‌</span></span><span b="" lang="FA" nazanin="" style="font-family:">داری وجود نداشت (313/0 </span><span dir="LTR" style="font-size:10.0pt">=</span> <span dir="LTR" style="font-size:10.0pt">P</span><span b="" lang="FA" nazanin="" style="font-family:">). از عوامل دموگرافیک موردمطالعه تنها رابطۀ بین سن و ابتلا به بیماری معنی</span><span dir="LTR" style="font-size:10.0pt"><span calibri="" style="font-family:">‌</span></span><span b="" lang="FA" nazanin="" style="font-family:">دار بود (029/0 </span><span dir="LTR" style="font-size:10.0pt">=</span> <span dir="LTR" style="font-size:10.0pt">P</span><span b="" lang="FA" nazanin="" style="font-family:">).</span></span></span></span></span></span><br> <span style="font-size:14pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجه</span></span></b><b><span dir="LTR" style="font-size:10.0pt"><span calibri="" style="font-family:">‌</span></span></b><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">گیری</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">: نتایج نشان داد بین حضور پلی</span></span><span dir="LTR" style="font-size:10.0pt"><span calibri="" style="font-family:">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مورفیسم</span></span><span dir="LTR" style="font-size:10.0pt">rs34173062 </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">&nbsp;در ژن <i>شارپین</i> و بروز بیماری آلزایمر در جمعیت ایرانیِ موردمطالعه رابطۀ معنی</span></span><span dir="LTR" style="font-size:10.0pt"><span calibri="" style="font-family:">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">داری یافت نشد. برای تأیید نتایج این پژوهش بررسی جمعیت</span></span><span dir="LTR" style="font-size:10.0pt"><span calibri="" style="font-family:">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">هایی با قومیت متفاوت و تعداد نمونۀ بیشتر توصیه می</span></span><span dir="LTR" style="font-size:10.0pt"><span calibri="" style="font-family:">‌</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">گردد.</span></span></span></span></span></span></span><br> &nbsp; <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Aim and Background:</span></span></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> However, the molecular mechanisms of Alzheimer&#39;s disease are unknown. Various genetic and environmental factors can cause Alzheimer&#39;s disease and are used to diagnose and predict the disease. The aim of this study was to investigate the presence of rs34173062 polymorphism in the <i>SHARPIN</i> gene and its association with triggering Alzheimer&#39;s disease in the Iranian patient population.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Materials and Methods: </span></span></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">This case-control study was conducted in 2021 on 50 people with Alzheimer&#39;s disease and 50 healthy controls. After DNA extraction from blood samples, genotyping was performed using the Tetra-ARMS PCR method and the data were statistically analyzed.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Results: </span></span></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">The genotype results for rs34173062 polymorphism showed that the frequencies of AA, AG and GG genotypes in the patient group were 62%, 10% and 28%, respectively, and in the control group were 48%, 18% and 34%, respectively. There was no significant association between rs34173062 polymorphism and the risk of Alzheimer&#39;s disease (P=0.313). Among the demographic factors studied, only the association between age and affected disease was significant (P=0.029).</span></span><b><span style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"></span></span></b></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Conclusion: </span></span></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">The results showed that there was no statistically significant association between the rs34173062 polymorphism of the <i>SHARPIN</i> gene and Alzheimer&#39;s disease in the Iranian population studied. To confirm the results of the present study, it is recommended to study populations from different societies and a larger number of samples.</span></span></span></span></span><br> &nbsp; پلی مرفیسم تک نوکلئوتیدی, بیماری آلزایمر,  ژن, واکنش زنجیره ایی پلیمراز. Single nucleotide polymorphism, Alzheimer's disease, gene, PCR. 71 80 http://ncmbjpiau.ir/browse.php?a_code=A-10-954-27&slc_lang=other&sid=1 Fariyal Pourbabaee فریال پور بابایی 100319475328460015348 100319475328460015348 No Department of Genetic, School of science, Islamic Azad University, North Tehran Branch, Tehran, Iran گروه ژنتیک، دانشکده علوم زیستی، دانشگاه آزاد اسلامی ،واحد تهران شمال، تهران، ایران Elham Siasi الهام سیاسی emi_biotech2006@yahoo.ca 100319475328460015349 100319475328460015349 Yes Department of Genetic, School of science, Islamic Azad University, North Tehran Branch, Tehran, Iran. گروه ژنتیک، دانشکده علوم زیستی، دانشگاه آزاد اسلامی ،واحد تهران شمال، تهران، ایران Robab Rafiei Tabatabaii رباب رفیعی طباطبایی 100319475328460015350 100319475328460015350 No Department of Genetic, School of science, Islamic Azad University, North Tehran Branch, Tehran, Iran. گروه ژنتیک، دانشکده علوم زیستی، دانشگاه آزاد اسلامی ،واحد تهران شمال، تهران، ایران