New Cellularand Molecular Biotechnology Journal
مجله تازه هاي بيوتكنولوژي سلولي و مولكولي
NCMBJ
Basic Sciences
http://ncmbjpiau.ir
1
admin
2228-5458
2228-6926
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8888
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fa
jalali
1404
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1
gregorian
2026
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1
fulltext
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سمیّت انتخابی و القای آپوپتوز توسط نانوذراتِ نقرۀ زیستیِ سنتزشده با عصارۀ نخلک ارهای در ردههای سلولیِ سرطانِ پستان و پروستات
Selective cytotoxicity and apoptosis induction by biosynthesized silver nanoparticles using )Serenoa repens( extract in breast and prostate cancer cell lines
سلولی و مولکولی
Cellular and molecular
مقاله پژوهشی
Research Article
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">سابقه و هدف: </span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">نانوذرات نقره</span></span> <span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">(AgNPs) </span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">بهدلیل خواص ضدسرطانی شناخته شدهاند و عصارۀ گیاه <i>نخل</i> </span></span><i><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">ارهای</span></span></i> <span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">(</span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">Serenoa</span></span></i><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif"> <i>repens</i></span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">)</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">نیز اثرهای مهاری بر رشد سلولهای سرطانی دارد. این مطالعه به بررسی سمیّت انتخابی نانوذرات نقرۀ سنتزشده با عصارۀ <i>نخل</i> <i>ارهای</i> بر سلولهای سرطانی پستان</span></span> <span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">(MCF-7)</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">و پروستات</span></span> <span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">(PC-3) </span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">در مقایسه با سلولهای نرمال</span></span> <span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">MCF-10A</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">و</span></span> <span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">RWPE-1</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">میپردازد</span></span><span dir="LTR" style="font-size:12.0pt">.</span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">مواد و روشها</span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">: نانوذرات نقره با عصارۀ آبی <i>نخل</i> <i>ارهای</i> تهیه شد. اثر آنها بر بقای سلولی در زمانهای </span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">۲۴</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">، </span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">۴۸</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""> و </span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">۷۲</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""> ساعت با آزمون</span></span> <span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">MTT</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">بررسی گردید. مرگ سلولی و آپوپتوز با استفاده از آنالیز</span></span> <span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">Annexin V/PI</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">ارزیابی شد. سلولها با غلظتهای 10 تا 80</span></span> <span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">µg/mL </span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">تیمارشده و مقدار</span></span> <span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">IC<sub>50</sub></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">تعیین شد. دادهها با آزمونهای آماری تحلیل شدند</span></span><span dir="LTR" style="font-size:12.0pt">.</span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">یافته ها</span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">: نانوذراِت سنتزشده اثرهای سمّی وابسته به دوز بر سلولهای</span></span> <span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">MCF-7</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">و</span></span> <span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">PC-3</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">داشتند و مقادیر</span></span> <span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">IC<sub>50</sub></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">بهترتیب </span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">۲۵</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""> و </span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">۵۰</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""> میکرومول تعیین شد. تحلیل رگرسیون خطی کاهشِ معنیدار بقای سلولهای سرطانی در مقایسه با سلولهای نرمال را تأیید کرد </span></span> <span dir="LTR" style="font-size:12.0pt">.</span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman",serif">(p<0.05)</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">نتیجهگیری: </span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">نانوذراتِ نقرۀ حاصل از عصارۀ <i>نخل</i> <i>ارهای</i> قابلیت هدفگیری انتخابیِ سلولهای سرطانی را دارند و آسیب کمتری به سلولهای طبیعی وارد میکنند، که نشاندهندۀ پتانسیلِ درمانیِ بالای آنها در سرطانهای پستان و پروستات است. تحقیق</span></span><span dir="LTR" style="font-size:12.0pt"></span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin"">های بیشتری برای تعیین دوز بهینه و بررسیِ دقیقتر مکانیسمهای آپوپتوز موردنیاز است</span></span><span dir="LTR" style="font-size:12.0pt">.</span><span lang="AR-SA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif">Aim and Background:</span></span></b><span style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"> Silver nanoparticles (AgNPs) are known for their anticancer properties, while <i>Serenoa repens</i> (Saw Palmetto) extract has demonstrated inhibitory effects on cancer cell growth. This study investigates the selective cytotoxicity of AgNPs synthesized with <i>S. repens</i> extract on breast (MCF-7) and prostate (PC-3) cancer cell lines, compared to normal cells (MCF-10A and RWPE-1).</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif">Materials and Methods:</span></span></b><span style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"> AgNPs were synthesized using an aqueous extract of <i>S. repens</i>. Cell viability was assessed at 24, 48, and 72 hours using the MTT assay. Apoptosis and cell death were analyzed through Annexin V/PI staining. Cells were treated with 10–80 µg/mL of AgNPs, and IC<sub>50</sub> values were calculated. Statistical analyses were used to compare group differences.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif">Results:</span></span></b><span style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"> The synthesized AgNPs exhibited dose-dependent cytotoxic effects on MCF-7 and PC-3 cells, with IC<sub>50</sub> values of 25 and 50 μM, respectively. Linear regression analysis confirmed a significant decrease in cancer cell viability with increasing nanoparticle concentrations, while minimal effects were observed on normal cell lines (p<0.05).</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif">Conclusion:</span></span></b><span style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"> AgNPs synthesized with <i>S. repens</i> extract selectively target cancer cells while sparing normal cells, indicating high therapeutic potential against breast and prostate cancers. Further studies are needed to determine optimal dosing and clarify apoptotic mechanisms.</span></span></span></span></span><br>
نانوذرات نقره, نخل ارهای, سرطان پستان, سرطان پروستات, آپوپتوز, سمیّت انتخابی
Silver Nanoparticles, Serenoa Repens, Breast Cancer, Prostate Cancer, Apoptosis, Selective Cytotoxicity
83
100
http://ncmbjpiau.ir/browse.php?a_code=A-10-2084-8&slc_lang=fa&sid=1
Behzad
Amraei
بهزاد
امرائی
100319475328460015763
100319475328460015763
Yes
Assistant Professor, Department of Biology, Payame Noor University, Tehran, Iran;
استادیار گروه زیستشناسی، دانشگاه پیام نور، تهران، ایران؛