New Cellularand Molecular Biotechnology Journal
مجله تازه هاي بيوتكنولوژي سلولي و مولكولي
NCMBJ
Basic Sciences
http://ncmbjpiau.ir
1
admin
2228-5458
2228-6926
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8888
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fa
jalali
1390
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gregorian
2011
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online
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fulltext
fa
جداسازی و شناسایی یک باکتری بومی تولید کننده بیوپلیمر پلی هیدروکسی آلکانوات (PHA) و بررسی تولید آن تحت شرایط مختلف رشد سلول
Isolation and characterization of a PHA-producing bacterial strain: biopolymer production under different growth conditions
میکروبیولوژی
Microbiology
مقاله پژوهشی
Research Article
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<!--stripped--></div><div style="text-align: justify"> </div><p style="margin: 0in 2.15pt 0.0001pt 0in line-height: 120% vertical-align: middle direction: rtl unicode-bidi: embed font-family: times new roman,times,serif text-align: justify" dir="rtl" class="MsoNormal"><font size="2"><b><span lang="FA" style="font-size: 12pt line-height: 120% color: black">سابقه وهدف:</span></b><span lang="FA" style="font-size: 12pt line-height: 120% color: black"> امروزه با توجه به مصرف بسیار زیاد
پلیمرهای مقاوم به تخریب زیستی از جمله انواع پلاستیک ها در بسته بندی مواد غذایی،
کالا و همچنین استفاده از کیسه های پلاستیکی برای جابجایی و حمل زباله مشکل بزرگ
تجمع ضایعات پلیمری در طبیعت به وجود آمده است. یکی از راه ها برای حل این مشکل
تولید پلیمر های زیست تخریب پذیر می باشد</span><span dir="ltr"></span><span style="font-size: 12pt line-height: 120% color: black" dir="ltr"><span dir="ltr"></span>.</span><span dir="rtl"></span><span style="font-size: 12pt line-height: 120% color: black"><span dir="rtl"></span> <span lang="FA"></span></span><b><span lang="FA" style="font-size: 12pt line-height: 120% color: black"><br></span></b></font></p><p style="margin: 0in 2.15pt 0.0001pt 0in line-height: 120% vertical-align: middle direction: rtl unicode-bidi: embed font-family: times new roman,times,serif text-align: justify" dir="rtl" class="MsoNormal"><font size="2"><b><span lang="FA" style="font-size: 12pt line-height: 120% color: black">مواد و روش ها: </span></b><span lang="FA" style="font-size: 12pt line-height: 120% color: black">باکتری<span> </span>تولید کننده پلیمر پلی هیدروکسی آلکانوآت </span><span dir="ltr"></span><span style="font-size: 12pt line-height: 120% color: black" dir="ltr"><span dir="ltr"></span>(PHA)</span><span dir="rtl"></span><span lang="FA" style="font-size: 12pt line-height: 120% color: black"><span dir="rtl"></span> از نمونه های محیطی جدا
سازی شد. این سویه باکتریایی با روش های شیمیایی و مولکولی از طریق تعیین توالی
ژن<span> </span></span><span dir="ltr"></span><span style="font-size: 12pt line-height: 120% color: black" dir="ltr"><span dir="ltr"></span>16s rDNA</span><span dir="rtl"></span><span lang="FA" style="font-size: 12pt line-height: 120% color: black"><span dir="rtl"></span> شناسایی
گردید. تولید </span><span style="font-size: 12pt line-height: 120% color: black" dir="ltr">PHA</span><span dir="rtl"></span><span lang="FA" style="font-size: 12pt line-height: 120% color: black"><span dir="rtl"></span> توسط این باکتری با استفاده از
کروماتوگرافی گازی و ستون </span><span style="font-size: 12pt line-height: 120% color: black" dir="ltr">HP-5</span><span dir="rtl"></span><span lang="FA" style="font-size: 12pt line-height: 120% color: black"><span dir="rtl"></span> تحت شرایط مختلف کشت
اندازه گیری شد.<span> </span></span></font></p><div style="text-align: justify"> </div><p style="margin: 0in 2.15pt 0.0001pt 0in line-height: 120% vertical-align: middle direction: rtl unicode-bidi: embed font-family: times new roman,times,serif text-align: justify" dir="rtl" class="MsoNormal"><font size="2"><b><span lang="FA" style="font-size: 12pt line-height: 120% color: black">یافته ها:</span></b><span lang="FA" style="font-size: 12pt line-height: 120% color: black"> در این مطالعه باکتری بومی تولید
کننده </span><span style="font-size: 12pt line-height: 120% color: black" dir="ltr">PHA</span><span dir="rtl"></span><span lang="FA" style="font-size: 12pt line-height: 120% color: black"><span dir="rtl"></span> بعنوان </span><span style="font-size: 12pt line-height: 120% color: black" dir="ltr">Bacillus thuringiensis ZK 86</span><span dir="rtl"></span><span lang="FA" style="font-size: 12pt line-height: 120% color: black"><span dir="rtl"></span> شناسایی
گردید. شرایط مناسب برای تولید این پلیمر دمای </span><span style="font-size: 12pt line-height: 120% color: black" dir="ltr">C</span><span style="font-size: 12pt line-height: 120% color: black" dir="ltr">º</span><span dir="rtl"></span><span lang="FA" style="font-size: 12pt line-height: 120% color: black"><span dir="rtl"></span> 37، 7=</span><span style="font-size: 12pt line-height: 120% color: black" dir="ltr">pH</span><span dir="rtl"></span><span lang="FA" style="font-size: 12pt line-height: 120% color: black"><span dir="rtl"></span> و هوادهی در
150</span><span dir="ltr"></span><span style="font-size: 12pt line-height: 120% color: black" dir="ltr"><span dir="ltr"></span>= rpm</span><span dir="rtl"></span><span lang="FA" style="font-size: 12pt line-height: 120% color: black"><span dir="rtl"></span> تعیین گردید.
تحت این شرایط بیشترین میزان تولید حدود<span> </span>367/0 گرم در لیتر با منبع گلوکز پس از کشت به مدت 72 ساعت بدست آمد و 35%
از وزن خشک سلولی را پلیمر تشکیل داد. </span></font></p><div style="text-align: justify"> </div><p style="margin: 0in 2.15pt 0.0001pt 0in line-height: 120% vertical-align: middle direction: rtl unicode-bidi: embed font-family: times new roman,times,serif text-align: justify" dir="rtl" class="MsoNormal"><font size="2"><b><span lang="FA" style="font-size: 12pt line-height: 120% color: black">نتیجه گیری:</span></b><span lang="FA" style="font-size: 12pt line-height: 120% color: black"> این مقاله اولین گزارش از تولید </span><span style="font-size: 12pt line-height: 120% color: black" dir="ltr">PHA</span><span dir="rtl"></span><span lang="FA" style="font-size: 12pt line-height: 120% color: black"><span dir="rtl"></span> توسط سویه
باسیلوس تورینجنسیس بومی ایران می باشد که در طی آن شناسایی باکتری تولید کننده و
روش های تولید و اندازی گیری میزان و نوع پلیمر تولید شده مورد ارزیابی قرار
گرفتند. </span></font></p><div style="text-align: justify"> </div>
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<!--stripped--><p style="margin-bottom: 0.0001pt text-align: justify line-height: 150% vertical-align: middle" class="MsoNormal"><b><span style="font-size: 14pt line-height: 150% font-family: "Times New Roman","serif" color: black">Aim and Background</span></b><b><span style="font-size: 12pt line-height: 150% font-family: "Times New Roman","serif" color: black">. </span></b><span style="font-size: 12pt line-height: 150% font-family: "Times New Roman","serif" color: black">The excessive applications
of non-biodegradable polymers, for examples in packaging, disposable utensils
and plastic bags of waste materials, have resulted in the environmental
accumulation of such polymers. The substitution of these polymers with biodegradable
ones seems a promising solution to this problem.<span> </span></span></p>
<p style="margin-bottom: 0.0001pt text-align: justify line-height: 150% vertical-align: middle" class="MsoNormal"><b><span style="font-size: 14pt line-height: 150% font-family: "Times New Roman","serif" color: black">Materials and Methods</span></b><b><span style="font-size: 12pt line-height: 150% font-family: "Times New Roman","serif" color: black">. </span></b><span style="font-size: 12pt line-height: 150% font-family: "Times New Roman","serif" color: black">A polyhydroxyalkanoate (PHA)
producing bacterium was isolated from environmental samples. This bacterial
strain was identified by biochemical and molecular methods using 16s rDNA
sequencing. The PHA production by this bacterium was quantified using gas
chromatography with an HP-5 column.<span> </span></span></p>
<p style="margin-bottom: 0.0001pt text-align: justify line-height: 150% vertical-align: middle" class="MsoNormal"><b><span style="font-size: 14pt line-height: 150% font-family: "Times New Roman","serif" color: black"> Results</span></b><b><span style="font-size: 12pt line-height: 150% font-family: "Times New Roman","serif" color: black">. </span></b><span style="font-size: 12pt line-height: 150% font-family: "Times New Roman","serif" color: black">In this study, the native
PHA producing bacterium with most similarity to <i>Bacillus thuringiensis</i>
was identified and designated as ZK 86. The optimum condition for PHA
production was 37 </span><span style="font-size: 12pt line-height: 150% font-family: "Times New Roman","serif" color: black">º</span><span style="font-size: 12pt line-height: 150% font-family: "Times New Roman","serif" color: black">C, pH 7 and aeration at 150 rpm.<span> </span>Under the mentioned condition, the maximum production accounting for 35%
of dry cell weight was achieved with glucose as carbon source after 72 h.</span></p><b><span style="font-size: 14pt line-height: 150% font-family: "Times New Roman","serif" color: black">Conclusion</span></b><b><span style="font-size: 12pt line-height: 150% font-family: "Times New Roman","serif" color: black">.</span></b><span style="font-size: 12pt line-height: 150% font-family: "Times New Roman","serif" color: black"> To our knowledge this
article is the first report on PHA production by an Iranian native <i>Bacillus
thuringiensis</i> strain<i>. </i>Through this study, the identification of the
isolate and protocols for production and identification of the polymers were
set up. </span>
<p style="margin-bottom: 0.0001pt line-height: 120% vertical-align: middle" class="MsoNormal"><b><span style="font-size: 14pt line-height: 120% font-family: "Times New Roman","serif" color: black">Key words</span></b><b><span style="font-size: 12pt line-height: 120% font-family: "Times New Roman","serif" color: black">. </span></b><span style="font-size: 12pt line-height: 120% font-family: "Times New Roman","serif" color: black">Biodegradable polymer,<i> Bacillus thuringiensis</i>,
Polyhydroxylalkanoate. Innate Immunity activated via<span> </span>siRNA.</span></p>
<p style="margin-bottom: 0.0001pt text-align: justify line-height: 120% vertical-align: middle" class="MsoNormal"><b><span style="font-size: 12pt line-height: 120% font-family: "Times New Roman","serif" color: black"> </span></b></p>
باسیلوس، پلی هیدروکسی آلکانوات، پلیمر زیست تخریب پذیر
Biodegradable polymer, Bacillus thuringiensis, Polyhydroxylalkanoate. Innate Immunity activated via siRNA
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http://ncmbjpiau.ir/browse.php?a_code=A-10-36-3&slc_lang=fa&sid=1
Hossein
Shahbani Zahiri
حسین
شهبانی
Shahabanih@yahoo.com
1003194753284600595
1003194753284600595
Yes
Islamic Azad University, Parand Branch
دانشگاهآازداسلامی واحد پرند
Zahra
Goudarzi
زهرا
گودرزی
1003194753284600596
1003194753284600596
No
Islamic Azad University, Science & Research Branch
دانشگاه آزاد اسلامی واحد علوم و تحقیقات تهران
Mohamad
Chamani
محمد
چمنی
1003194753284600597
1003194753284600597
No
Islamic Azad University, Science & Research Branch
دانشگاه آزاد اسلامی واحد علوم و تحقیقات تهران
Kambiz
Akbari Noghabi
کامبیز
اکبری نوقابی
1003194753284600598
1003194753284600598
No
National Institute of Genetic Engineering & Biotechnology,
پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری، تهران