@article{ author = {Mohajeri, nasrin and kazemi, bahram}, title = {Innate Immunity activated via siRNA}, abstract ={Normal 0 false false false EN-US X-NONE AR-SA /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal" mso-tstyle-rowband-size:0 mso-tstyle-colband-size:0 mso-style-noshow:yes mso-style-priority:99 mso-style-qformat:yes mso-style-parent:"" mso-padding-alt:0in 5.4pt 0in 5.4pt mso-para-margin-top:0in mso-para-margin-right:0in mso-para-margin-bottom:10.0pt mso-para-margin-left:0in line-height:115% mso-pagination:widow-orphan font-size:11.0pt font-family:"Calibri","sans-serif" mso-ascii-font-family:Calibri mso-ascii-theme-font:minor-latin mso-fareast-font-family:"Times New Roman" mso-fareast-theme-font:minor-fareast mso-hansi-font-family:Calibri mso-hansi-theme-font:minor-latin mso-bidi-font-family:Arial mso-bidi-theme-font:minor-bidi} Aim and Background .RNA interference has the role of gene silencing process that is triggered by double-stranded RNAs. Common to all cell types, is the production of 21-24 nucleotide small interfering RNA (siRNA), which guides the RNA-induced silencing complex (RISC) to identify and cleave target mRNA sequences. siRNA duplex are potent activators of innate immune system that induce high levels of cytokines and interferon. Toll-like receptors trigger the first line of innate immune response that is mediated by transcriptional induction of a large number of cellular genes. siRNA interact with cytoplasmic RNA sensors such as: Retinoic - acid Inducible Gene -I (RIG-I), RNA-Activated protein kinase R(PKR), Interferon Regulatory Factor (IRF) and 2'-5' oligo adenylate synthetase (2'-5'OAS), Nuclear Factor kappa B cell(NF-κB). siRNA modification are on the sugar, phosphate linkage, base, overhangs and termini nucleotides leading to increase half-life siRNA. This review article describes gene silencing and innate immune caused by siRNA.   }, Keywords = {dsRNA, siRNA, Innate immunity response}, volume = {1}, Number = {3}, pages = {7-15}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-40-en.html}, eprint = {http://ncmbjpiau.ir/article-1-40-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2011} } @article{ author = {Bakhsikhaniki, Gholamreza and Asgharisana, Farzin and Gaibi, Shahsanam}, title = {study of the role of common bacterial etiology in neonatal sepsis in Urumiah Shahid}, abstract ={Normal 0 false false false EN-US X-NONE AR-SA /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal" mso-tstyle-rowband-size:0 mso-tstyle-colband-size:0 mso-style-noshow:yes mso-style-priority:99 mso-style-qformat:yes mso-style-parent:"" mso-padding-alt:0in 5.4pt 0in 5.4pt mso-para-margin-top:0in mso-para-margin-right:0in mso-para-margin-bottom:10.0pt mso-para-margin-left:0in line-height:115% mso-pagination:widow-orphan font-size:11.0pt font-family:"Calibri","sans-serif" mso-ascii-font-family:Calibri mso-ascii-theme-font:minor-latin mso-fareast-font-family:"Times New Roman" mso-fareast-theme-font:minor-fareast mso-hansi-font-family:Calibri mso-hansi-theme-font:minor-latin mso-bidi-font-family:Arial mso-bidi-theme-font:minor-bidi} Aim and Background. The aim of this study was to assess the most common bacterial etiology in neonatal sepsis which has been carried out during the years 2005-2006 at Urumiah Shahid Arefian Hospital. Materials and Methods. In this cross-sectional study, after collecting blood samples from 402 newborns who were suspicious to sepsis and performing microbial culture and bacterial isolation, disk diffusion antibiotic susceptibility test was done according to the CLSI guidelines. Results. Overall, 36 positive cultures were acquired. The prevalence of sepsis were higher in the male infants (61/1%) than female (38/9%) (P=0) Among these, 63/9% were born with normal vaginal delivery and 36/1% were c-section delivered babies (P=0/093). The mean weight of 36 neonates was 2117837 gr and there was a significant relationship between low birth weight and prevalence of sepsis (p=0). The most common microorganisms of neonatal sepsis were coagulase negative staphylococci (50%) and klebsiella pneumoniea (19/4%). The gram-positive isolates showed the highest sensitivity and resistance rates to vancomycin and to Ampicillin, respectively. The highest sensitivity and resistance rates for the gram negatives were towards ciprofloxacin and cefotaxime, respectively. Low birth weight and prematurity were the major risk factors of sepsis. Conclusion. With respect to increasing prevalence of neonatal sepsis with coagulase negative staphylococci, in this study and similar studies, with appropriate antibiotical therapy according to antibiogram results, with considering hygienic principles in hospitals and prenatal care, we may able to decrease mortality and morbidity of neonatal sepsis.}, Keywords = {Neonatal sepsis, bacterial agent, Antibiotic resistance, Prematurity}, volume = {1}, Number = {3}, pages = {17-21}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-41-en.html}, eprint = {http://ncmbjpiau.ir/article-1-41-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2011} } @article{ author = {ShahbaniZahiri, Hossein and Goudarzi, Zahra and Chamani, Mohamad and AkbariNoghabi, Kambiz}, title = {Isolation and characterization of a PHA-producing bacterial strain: biopolymer production under different growth conditions}, abstract ={Normal 0 false false false EN-US X-NONE AR-SA /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal" mso-tstyle-rowband-size:0 mso-tstyle-colband-size:0 mso-style-noshow:yes mso-style-priority:99 mso-style-qformat:yes mso-style-parent:"" mso-padding-alt:0in 5.4pt 0in 5.4pt mso-para-margin-top:0in mso-para-margin-right:0in mso-para-margin-bottom:10.0pt mso-para-margin-left:0in line-height:115% mso-pagination:widow-orphan font-size:11.0pt font-family:"Calibri","sans-serif" mso-ascii-font-family:Calibri mso-ascii-theme-font:minor-latin mso-fareast-font-family:"Times New Roman" mso-fareast-theme-font:minor-fareast mso-hansi-font-family:Calibri mso-hansi-theme-font:minor-latin mso-bidi-font-family:Arial mso-bidi-theme-font:minor-bidi} Aim and Background. The excessive applications of non-biodegradable polymers, for examples in packaging, disposable utensils and plastic bags of waste materials, have resulted in the environmental accumulation of such polymers. The substitution of these polymers with biodegradable ones seems a promising solution to this problem.  Materials and Methods. A polyhydroxyalkanoate (PHA) producing bacterium was isolated from environmental samples. This bacterial strain was identified by biochemical and molecular methods using 16s rDNA sequencing. The PHA production by this bacterium was quantified using gas chromatography with an HP-5 column.   Results. In this study, the native PHA producing bacterium with most similarity to Bacillus thuringiensis was identified and designated as ZK 86. The optimum condition for PHA production was 37 ºC, pH 7 and aeration at 150 rpm. Under the mentioned condition, the maximum production accounting for 35% of dry cell weight was achieved with glucose as carbon source after 72 h.Conclusion. To our knowledge this article is the first report on PHA production by an Iranian native Bacillus thuringiensis strain. Through this study, the identification of the isolate and protocols for production and identification of the polymers were set up. Key words. Biodegradable polymer, Bacillus thuringiensis, Polyhydroxylalkanoate. Innate Immunity activated via siRNA.  }, Keywords = { Biodegradable polymer, Bacillus thuringiensis, Polyhydroxylalkanoate. Innate Immunity activated via siRNA}, volume = {1}, Number = {3}, pages = {23-32}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-42-en.html}, eprint = {http://ncmbjpiau.ir/article-1-42-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2011} } @article{ author = {Tafvizi, Farzaneh and Tajabadiebrahimi, Maryam and HeydariNasrabadi, Mitra and Bahrami, Ho}, title = {Detection of genetic diversity of lactobacillus species isolated from different traditional dairy products by RAPD markers}, abstract ={Normal 0 false false false EN-US X-NONE AR-SA /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal" mso-tstyle-rowband-size:0 mso-tstyle-colband-size:0 mso-style-noshow:yes mso-style-priority:99 mso-style-qformat:yes mso-style-parent:"" mso-padding-alt:0in 5.4pt 0in 5.4pt mso-para-margin-top:0in mso-para-margin-right:0in mso-para-margin-bottom:10.0pt mso-para-margin-left:0in line-height:115% mso-pagination:widow-orphan font-size:11.0pt font-family:"Calibri","sans-serif" mso-ascii-font-family:Calibri mso-ascii-theme-font:minor-latin mso-fareast-font-family:"Times New Roman" mso-fareast-theme-font:minor-fareast mso-hansi-font-family:Calibri mso-hansi-theme-font:minor-latin mso-bidi-font-family:Arial mso-bidi-theme-font:minor-bidi} Aim and background. The genus Lactobacillus encompasses a diverse assemblage of Gram-positive, catalase-negative, non-spore-forming, rod-shaped organisms. Over 90 species are currently described. Several strains of probiotic lactobacilli have been incorporated in a wide range of products for human. As the probiotic capacities are strain-dependent, methods for reliable identification of lactobacilli at the strain level are of great importance. Regarding biochemical methods are time-consuming and often very ambiguous the molecular methods are more accurate. The aim of present study is typing of lactobacillus species isolated from different traditional dairy products by RAPD markers. Materials and Methods. The 20 strains used in this study were isolated from different sources. Samples were grown in MRS broth then total genomic DNA was extracted. RAPD markers were used for the detection of genetic diversity.  Results. Out of 20 primers used 19 primers produced reproducible bands. The primers generated 225 RAPD loci, of which 219 were polymorph, 6 were monomorph and 63 were unique. NJ and UPGMA clustering methods performed on molecular data by NTSYS-PC software. Similar results are obtained by two methods.  Conclusion. UPGMA clustering methods were performed on molecular data. Two genotypes of k2l3 and y1m4 are grouped together in two dendrograms. In UPGMA dendrogram two major clusters A and B were observed. Cluster A was divided in to2 subcluster by c6m3،y1l4، y2f3 and c1d2 genotypes. In cluster B, 2 subclusters by 6 genotypes (c2h1، d3b1، y2c4، k2l3و y1m4 and p) were observed. Key words. Lactobacillus strain, genetic diversity, traditional dairy products, RAPD markers, UPGMA dendrogram.}, Keywords = {Lactobacillus strain, genetic diversity, traditional dairy products, RAPD markers, UPGMA dendrogram}, volume = {1}, Number = {3}, pages = {33-42}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-43-en.html}, eprint = {http://ncmbjpiau.ir/article-1-43-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2011} } @article{ author = {Ganavati, Farangis}, title = {Cytogenetical survey of some Iranian Onobrychis species}, abstract ={Normal 0 false false false EN-US X-NONE AR-SA /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal" mso-tstyle-rowband-size:0 mso-tstyle-colband-size:0 mso-style-noshow:yes mso-style-priority:99 mso-style-qformat:yes mso-style-parent:"" mso-padding-alt:0in 5.4pt 0in 5.4pt mso-para-margin-top:0in mso-para-margin-right:0in mso-para-margin-bottom:10.0pt mso-para-margin-left:0in line-height:115% mso-pagination:widow-orphan font-size:11.0pt font-family:"Calibri","sans-serif" mso-ascii-font-family:Calibri mso-ascii-theme-font:minor-latin mso-fareast-font-family:"Times New Roman" mso-fareast-theme-font:minor-fareast mso-hansi-font-family:Calibri mso-hansi-theme-font:minor-latin mso-bidi-font-family:Arial mso-bidi-theme-font:minor-bidi}  Aim and background.Sainfoin (Onobrychis) in Iran with 63 annual and perennial species that are distributed in different climatic regions of distribution. Some species of this genus are used for value of forage and erosion control and plants to attract visitors as honeybees. These species have high genetic diversity and can be used as valuable genetic resources in breeding of cultivated species. Materials and Methods. Nine species of of sainfoin were collected from natural habitats across Iran. Number and size of chromosomes as well as karyotypic formula of the populations were measured and studied using their root tip meristemes. Results.The basic chromosome number varied between x=7 and x=8 and their chromosomal type was metacentric and sub-metacentric. According to average genoms length, the highest was for O. scrobiculata (24.26) and the lowest belongs to O. lunata (14.76). According to Stebbins' classification, O. heliocarpa and O. lunata are classified as symmetric class of A and others as B. O. heliocarpa with 16m formula had the most total form percentage (44.856) and lowest intra chromosal asymmetrical and O. oxyptera with 2m+ 14sm had lowest TF% (32.552) and the most asymmetrical karyotype. Dendrogram results from cluster analysis (Ward) based on the karyptipic parameters species were classified in to two classes. The highest distance was obtained between O. oxyptera and O. heliocarpa and the lowest metric distance value was obtained between O. buhseana and O. atropatana. Conclusion. The results of this analysis for species classification and selection of species were close to each other as to perform crosses between species   }, Keywords = {Cluster analysis, Karyotype, Onobrychis. }, volume = {1}, Number = {3}, pages = {43-49}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-45-en.html}, eprint = {http://ncmbjpiau.ir/article-1-45-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2011} } @article{ author = {Onsory, Khadigeh and Navabi, samaneh}, title = {The effect of Environmental Factors in the incidence of Brain Cancer}, abstract ={Normal 0 false false false EN-US X-NONE AR-SA /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal" mso-tstyle-rowband-size:0 mso-tstyle-colband-size:0 mso-style-noshow:yes mso-style-priority:99 mso-style-qformat:yes mso-style-parent:"" mso-padding-alt:0in 5.4pt 0in 5.4pt mso-para-margin-top:0in mso-para-margin-right:0in mso-para-margin-bottom:10.0pt mso-para-margin-left:0in line-height:115% mso-pagination:widow-orphan font-size:11.0pt font-family:"Calibri","sans-serif" mso-ascii-font-family:Calibri mso-ascii-theme-font:minor-latin mso-fareast-font-family:"Times New Roman" mso-fareast-theme-font:minor-fareast mso-hansi-font-family:Calibri mso-hansi-theme-font:minor-latin mso-bidi-font-family:Arial mso-bidi-theme-font:minor-bidi} Aim and Background: This study was aimed to determine the effect of different environmental factors in the incidence of brain cancer in patients admitted in Shohada Hospital Tehran. Dietary habits, vitamins, age, sex, education, occupation income, weight, cigarette smoking, alcohol consumption and family history were concerned as risk factors.  Materials and Methods.The data were analyzed using the computer software SPSS for windows (version 11). The case-control study was conducted in 100 brain cancer patients and 100 healthy people as control. Results. An increased association was found with patients using non-vegetables such as red and white meat compared to control group which were mostly vegetarian. Low risk of brain cancer was also found among people using different vitamins daily. No association was observed with age and sex factors and risk of brain cancer. The analyzed factors like low income and low level of education also resulted in positive association with brain cancer. We also found statistically significant association between smoking habits, alcohol consumption and positive family history with brain cancer risk. Occupation was also found to be an important risk factor in this case-control study as patients working with radiography or X-ray instruments, showed a high frequency.  Conclusion. This study shows that some of the environmental factors are important in increasing the risk of brain cancer.  }, Keywords = {Brain Cancer, Environmental Factors}, volume = {1}, Number = {3}, pages = {51-63}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-46-en.html}, eprint = {http://ncmbjpiau.ir/article-1-46-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2011} } @article{ author = {Bakhshikhaniki, Gholamreza and Javadi, Sedigheh and Mehdikhani, Parviz and Tahmasebi, Davo}, title = {Investigation of drought stress effects on some quantity and quality characteristics of new eugenics sugar beet genotypes}, abstract ={Normal 0 false false false EN-US X-NONE AR-SA /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal" mso-tstyle-rowband-size:0 mso-tstyle-colband-size:0 mso-style-noshow:yes mso-style-priority:99 mso-style-qformat:yes mso-style-parent:"" mso-padding-alt:0in 5.4pt 0in 5.4pt mso-para-margin-top:0in mso-para-margin-right:0in mso-para-margin-bottom:10.0pt mso-para-margin-left:0in line-height:115% mso-pagination:widow-orphan font-size:11.0pt font-family:"Calibri","sans-serif" mso-ascii-font-family:Calibri mso-ascii-theme-font:minor-latin mso-fareast-font-family:"Times New Roman" mso-fareast-theme-font:minor-fareast mso-hansi-font-family:Calibri mso-hansi-theme-font:minor-latin mso-bidi-font-family:Arial mso-bidi-theme-font:minor-bidi} Aim and Background. In order to study the effect of drought stress on some quantity and quality characteristics of sugar beet genotypes a field experiment was carried out at Agricultural Research Station of Khoy in 2009. Materials and Methods. Treatments was arranged in strip block experiments based on completely randomized block design with three replications. Three sugar beet hybrid varieties were as Rasoul, Shirin and 7112.   Results. The results showed that the water stress on root yield, white sugar content, sugar content, root length, fresh and dry weight of leaves, petioles and roots were significant. Effects of cultivar on most traits studied were significant, and Rasoul was significantly higher than others of root yield and white sugar yield. The interaction between cultivar and water stress was not significant. Due to stress and free- stress environments, Shoot fresh weight reduction showed the water storage in cultivars is non-equal. Decrease in shoot dry weight and total dry weight indicate that drought stress have reducing affects on the growth of shoot parts and total dry weight.Conclusion. Noticeable point in the results was that the traits sugar content and white sugar yield, impurities of nitrogen, sodium and potassium, and molasses sugar in two environments revealed no significant difference. }, Keywords = {Sugar Beet; Plant Density; Water Stress; Yield}, volume = {1}, Number = {3}, pages = {65-74}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-47-en.html}, eprint = {http://ncmbjpiau.ir/article-1-47-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2011} } @article{ author = {Cheshmi, Hami}, title = {Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers}, abstract ={Normal 0 false false false EN-US X-NONE AR-SA /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal" mso-tstyle-rowband-size:0 mso-tstyle-colband-size:0 mso-style-noshow:yes mso-style-priority:99 mso-style-qformat:yes mso-style-parent:"" mso-padding-alt:0in 5.4pt 0in 5.4pt mso-para-margin-top:0in mso-para-margin-right:0in mso-para-margin-bottom:10.0pt mso-para-margin-left:0in line-height:115% mso-pagination:widow-orphan font-size:11.0pt font-family:"Calibri","sans-serif" mso-ascii-font-family:Calibri mso-ascii-theme-font:minor-latin mso-fareast-font-family:"Times New Roman" mso-fareast-theme-font:minor-fareast mso-hansi-font-family:Calibri mso-hansi-theme-font:minor-latin mso-bidi-font-family:Arial mso-bidi-theme-font:minor-bidi} Aim and Background. Glioblastoma tumour cells release microvesicles (exosomes) containing mRNA, miRNA and angiogenic proteins. These microvesicles are taken up by normal host cells, such as brain microvascular endothelial cells.  Materials and methods. By incorporating an mRNA for a reporter protein into these microvesicles, we demonstrate that messages delivered by microvesicles are translated by recipient cells. These microvesicles are also enriched in angiogenic proteins and stimulate tubule formation by endothelial cells.Results. Tumour-derived microvesicles therefore serve as a means of delivering genetic information and proteins to recipient cells in the tumour environment. Glioblastoma microvesicles also stimulated proliferation of a human glioma cell line, indicating a self-promoting aspect. Messenger RNA mutant/variants and miRNAs characteristic of gliomas could be detected in serum microvesicles of glioblastoma patients. The tumour-specific EGFRvIII was detected in serum microvesicles from 7 out of 25 glioblastoma patients. Conclusion. The tumour-specific EGFRvIII was detected in serum microvesicles from 7 out of 25 glioblastoma patients. Thus, tumour-derived microvesicles may provide diagnostic information and aid in therapeutic decisions for cancer patients through a blood test. }, Keywords = {Glioblastoma, miRNA, EGFRvIII, microvesicle}, volume = {1}, Number = {3}, pages = {75-88}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-48-en.html}, eprint = {http://ncmbjpiau.ir/article-1-48-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2011} }