@article{ author = {Rohaninej, Hamide and Norouzi, Jamileh and FallahMehrabadi, Jalil and Mahdavi, Mehdi}, title = {Creating a human ScFv antibody library based on T-Vector against tetanus toxin}, abstract ={Aims and Background: Nowadays, monoclonal antibodies (mAbs) became powerful therapeutic and diagnostic tools. Due to showing minimum immunogenic reaction and their properties, human mAbs are important. The purpose of this study was to construct an immune antibody library from a vaccinated donor against tetanus toxin. Materials and Methods: A whole blood sample was taken from the donor who was vaccinated against tetanus toxoid. PBMC were isolated by using ficol. After RNA extraction, all variation of VH and VL regions by RT-PCR reactions were amplified and linked as a ScFv antibody. The amplicons were inserted in T-vector and transformed to E. coli DH5α strain, followed by an ELISA test. Plasmids were also extracted and sequenced. Results: cDNA quality was confirmed by using HPRT primers. To confirm PCR, insertion and transformation, gel electrophoresis and restriction enzymes digestion were applied. Positive clones were selected based on growth on LB agar which is the blue/white selection method. After plasmid extraction and DNA sequencing, the sequences were aligned using igBLAST at NCBI. The result was shown to have admissible similarity among antibody gene library nucleotide sequences and the antibody genes were deposited in this database. ELISA confirmed this data too. Conclusion: In this study, the immune human antibody library was constructed and confirmed using DNA sequencing and sequences alignment in NCBI database. ELISA test confirms antibody specifically. The next step is to screen the library to find an antibody specifically for tetanus toxin.}, Keywords = { Antibody library gene, ScFv, Tetanuss toxin, ELISA}, volume = {5}, Number = {17}, pages = {1-7}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-502-en.html}, eprint = {http://ncmbjpiau.ir/article-1-502-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Fatemi, Farzaneh and Najafizarrini, Hamid and Heydari, Parviz}, title = {Phylogenetic and Functional Study of SOS1 Gene to Different Plant Species}, abstract ={  Aim and Background: Soil salinity is one of the major abiotic stresses reducing yield crop. SOS (Salt overly sensitive) system in Arabidopsis thaliana act as cellular signaling system in salinity tolerance pathways. SOS1 has an important role in salinity tolerance. Materials and Methods: In this study, SOS1 gene sequence identified in various plants has been recorded in the databases such as Tair, EBI, NCBI, plant care and EXPASY. We use an in silico analysis in order to determine phylogenetic study and the homology of 13 plant species by bioedit, UPGMA and MEGA5 software. Results: The cluster analysis showed that sos1 protein sequences of these plants classified in three groups. The results of the evolutionary matrix showed that the most distance was between Aegilops tauschii and Arabidobsis thaliana (0.542) and the least distance was between Triticum aestivum and Aegilops tauschii (0.004). Promoter Sequence analysis of SOS1 gene showed that, the ABRE and CAAT boxes were found in all areas of plant promoter. Also, aligning protein sequences showed complete similarity in 15 Amino acids. Conclusion: Due to the presence of ABRE box activated in response to Abscisic acid and CAAT box and increases gene expression in all areas of plant promoter, suggests the potentiality of plants to cope with abiotic stresses. Complete similarity in 15 Amino acids shows conserved region during the evolution process and can have an important structural role in the protein's function and nucleotide sequences of those suggested to design primers.}, Keywords = {salinity, sos1, phylogenetic analysis, cluster analysis}, volume = {5}, Number = {17}, pages = {8-12}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-365-en.html}, eprint = {http://ncmbjpiau.ir/article-1-365-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {BakhshiKhaniki, Gholamreza and Ghorbanli, Mahlagha and Baloochi, Mobarakeh}, title = {Effects of artificial frost on seed germination of apricot}, abstract ={Aim and Background: Apricot is one of the economically important genera of woody plants in Iran and most of these important varieties grow in Azarbaijan region, because of its Mediterranean ecological factors. Apricot has a large stone seed. Seeds are naturally planted in early autumn and after a prolonged exposure to cold temperatures (winter), seeds germinate in the end of third month of spring or in the beginning of the summer taking sometimes 18 months. Germination occurs more rapidly after cold treatment (vernalization), thus it cannot germinate without prior cold exposure In. this study, an artificial way has been suggested this method significantly decreases duration of germination. Furthermore, it increases percentage of germination, because seeds in the garden maybe moulded by microorganisms of soil. Materials and Methods: In this study, 10 seeds of each type of apricot (control) were first planted in the garden and then 10 seeds of each type of apricot were transferred on whattman paper inside each petri dish. All petri dishes were incubated to germinate. The condition of germinator was: 16h day light and 8h dark period with 20˚C and 16˚C respectively. The humidity condition inside germinator was 65%. And the percentage and the rate of germination was measured up to 12 days. Results: The findings showed that the percentage of germination was increased significantly in seeds when compared with control. Also, hundred percent of germination was obtained in D. However, artificial frost affects germination percentage. Therefore, the AVG used germination rate calculation is suggested and Artificial cold period is advised to be done in germination in order to increase percentage and velocity of stone seed germination. Conclusions: It is suggested to use artificial germination for better germination.}, Keywords = {Apricot, artificial frost, germination percentage, velocity Germination vernalization}, volume = {5}, Number = {17}, pages = {13-19}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-37-en.html}, eprint = {http://ncmbjpiau.ir/article-1-37-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Ferdousi, Atousa and Shahhosseini, Mohammad hassan and Bayat, Mansour and Hashemi, Seyed Jamal and Ghahri, Mohamm}, title = {Molecular diagnosis of Fusarium solani in corneal samples from suspected herpetic keratitis cases}, abstract ={Aim and Background: Fusarium solani, may occur as a mixed infection with HSV and due to similarities in their clinical features, sometimes it leads to misdiagnosis. The aim of this research was the designation and optimization of PCR technique for the diagnosis of F. solani as the main etiological agent or as a mixed infection in corneal samples suspected of having HSV Materials and Methods: 100 samples of DNA from clinical cases (Include 65 negative and 35 positive samples for HSV), suspected to herpetic keratitis were collected. The PCR test for detecting F.solani in these samples, with specific primers ffuso1 and rfuso2 and mt cytb as the target gene with 330 bp product was optimized. The specificity and sensitivity of the test were then evaluated. Results: Among 65 negative samples for HSV, two samples were positive for Fusarium solani and the PCR 330 bp product were amplified in these two cases. The sensitivity of the test was one copy of Fusarium solani genome and specificity was 100%. Discussion:The results of PCR test show that, the cases suspected to herpetic keratitis are caused by Fusarium solani., Conclusion: Accurate diagnosis of ethological agent in the cases of clinical features similarity or mix infection, is possible by molecular diagnosis.}, Keywords = {Keratitis, Fusarium solani, PCR, HSV}, volume = {5}, Number = {17}, pages = {20-26}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-390-en.html}, eprint = {http://ncmbjpiau.ir/article-1-390-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Nikzad, Abouzar and Salehi, Seyed Younes and Bahrami, Ali and Arabian, Daryoush and Ghafari, Mohammad davood and Masumian, Mohammad rez}, title = {Comparison of fermentation time effect on phenazine 1-carboxylic acid (PCA) anti-corrosion antibacterial activity extracted from Pseudomonas aeruginosa MUT3 strain against steel corrosive bacteria}, abstract ={Aim and Background: The most destructive types of corrosion are biocorrosion which about 20-40 percent of corrosion recompense are related to it. Natural phenazines in secondary metabolites of bacteria have been receiving increasing attention in recent years due to their potential usage as antibiotics. Materials and Methods: Steel corrosive bacteria isolated from marine source as the main cause of sea structure corrosion was studied to assay anticorrosion antibacterial material effects on the activity of this microorganism. In vitro antibacterial production of phenazine 1-carboxylic acid (PCA) was evaluated for seven days in batch fermentation. Results: The experiments showed maximum mass production is related to sixth day with 0.313 g/l products, but the antibacterial activity was tested and the inhibition zone on the fourth day was bigger than the other days and analysis of high pressure liquid Chromatography (HPLC) indicates more purity of PCA on this day. Conclusion: According to the result, future increase in PCA production was achieved after four days fermentation. Therefore, in future prospects, we will try to formulate these compounds as anti-corrosive paints for industrial use.}, Keywords = {bio-corrosion, anti-bacterial phenazine, inhibition zone, chromatography, batch culture}, volume = {5}, Number = {17}, pages = {27-34}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-375-en.html}, eprint = {http://ncmbjpiau.ir/article-1-375-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Ghiasi, Mahdieh and Sheykhhasan1, Mohsen and TabatabaeiQomi, Reza and Kalhor, Naser and Mehdizadeh, Mohamm}, title = {The comparison of natural and synthetic scaffolds protective capabilities as a suitable growth medium for adipose-derived mesenchymal stem cells}, abstract ={Aim and Background: Tissue engineering is a science using advanced techniques to repair damaged tissue and cause their recovery and reconstruction. The success of this process requires the presence of optimal and effective factors. Among the important factors in this success is to use appropriate scaffolds with wonderful mechanical and biological properties to provide an optimal growth environment and appropriate condition for the differentiation of stem cells. In this research, the evaluation of PRP and PLGA scaffolds efficiency in producing suitable environment for growth of mesenchymal stem cells was carried out. Materials and Methods: In this study, after preparing PRP and PLGA scaffolds and isolating mesenchymal stem cells from adipose tissue, the cells were seeded separately into two scaffolds and after 48 hours of cell seeding, the cells viability was evaluated by MTT assay. Results: Our findings showed that both PRP and PLGA scaffolds were used as an ideal environment for the culture and proliferation of mesenchymal stem cells. Discussion: The obtained results of this study using MTT assay indicated that when the active PRP are used with mesenchymal stem cells, its efficiency was higher than PLGA. Conclusion: It seems that using PRP-derived scaffolds as a protective scaffold is better to be considered for mesenchymal stem cells growth to provide effective strategies for the development of tissue engineering and regenerative medicine.}, Keywords = {Tissue Engineering- PRP- PLGA- Mesenchymal Stem Cells}, volume = {5}, Number = {17}, pages = {35-40}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-397-en.html}, eprint = {http://ncmbjpiau.ir/article-1-397-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Rahmani, Abbas and Nejadsattari, Taher and Hamdi, Seayed Mohammad mehdi and Mehregan, Iraj and Assadi, Mostaf}, title = {Phylogenetic Study of Linaria (Plantaginaceae) Based on Chloroplastic DNA Sequences From Iran}, abstract ={Aim and Background: Genus Linaria Mill. Belonging to the Plantaginaceae family is categorized in Lamiales order. The habitat of this genus is the northern hemisphere and in some parts of Iran. The purpose of this study was to investigate the phylogeny and taxonomy relationships of the mentioned genus in Iran. Materials and Methods: Molecular studies were performed in accordance with the following procedure: 1-Total DNA extracted from leaves, 2-Amplification and sequencing of the genome of the chloroplast rpl32-trnL fragment area 3-Statistical analysis of evolutionary models to obtain and review the phylogeny of the studied groups,4- Interpretation of cladograms. Results: The data matrix consisted of 71 taxa and 702 characters in Parsimony analysis. The 485 characters are constant, 109 variable characters are parsimony-uninformative, The remaining 108 are potentially parsimony informative. Four major clades including A, B, C and D were detected in Phylogenetic tree analysis. Discussion: Monophyly of Linaria species was supported in this study. A basal divergence between species with winged and wingless seeds was clearly unsupported, implying the homoplasy of this trait. Conclusion: the results showed that Linaria species constitute a Monophylitic group. According to a set of morphological traits including entire, sessile, pinnately veined leaves terminal, bracteate, racemose inflorescences and spurred flowers monophyly of Linaria confirmed.}, Keywords = {Linaria, Parsimony Analysis, Taxonomy, Phylogeny }, volume = {5}, Number = {17}, pages = {41-50}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-491-en.html}, eprint = {http://ncmbjpiau.ir/article-1-491-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {GhaffarianKablu, Iraj and Ghiamirad, Mehdi and Mahdavi, Sam}, title = {Study of seroprevalence of cytomegalovirus in voluntary blood donors of Ardebil province}, abstract ={Introduction and Objective: Human cytomegalovirus infection has high incidence worldwide. This virus is an important mortality factor in some blood donors. The aim of this investigation was study of seroprevalence of anti-cytomegalovirus IgG and IgM in voluntary blood donors of Ardebil province. Materials and Methods: In this cross-sectional study after blood samples collection and serum separation, the specific anti-cytomegalovirus IgG and IgM antibodies were evaluated on sera using ELISA method and the relation of the obtained results with different parameters including gender, age, blood group and Rh factor were analyzed with appropriate statistical method using SPSS 17 version software. Results: From 182 collected sera, 180 samples were positive for anti-cytomegalovirus IgG antibody (98.9%) whereas only 10 samples were positive for IgM antibody (5.5%) and 1 sample was suspicious. There was not statistical significance between blood donor’s sexuality and IgG class anti-cytomegalovirus prevalence, meanwhile, concerning IgM class anti-cytomegalovirus prevalence, it showed statistical significance (P0.05). Conclusion: In recent study, the high seroprevalence of anti-cytomegalovirus IgG and IgM antibodies was found in voluntary blood donors of Ardebil province resemble of other parts of country.}, Keywords = {Cytomegalovirus, Seroprevalence, IgG, IgM}, volume = {5}, Number = {17}, pages = {51-56}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-399-en.html}, eprint = {http://ncmbjpiau.ir/article-1-399-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Alishiri, Athar and Rakhshandehroo, Farshad and Zamanizadeh, Hamidrez}, title = {Using viruses as carriers for drug delivery}, abstract ={Introduction: new nano-carrier platforms based on natural biological building blocks offer great promises in revolutionizing medicine. Materials and methods: The usage of specific protein cage structures such as virus-like particles (VLPs) for drug packaging and targeted delivery has attracted much attention. Results: versatile chemical and genetic modifications on the outer surfaces and inner cavities of VLPs facilitate the preparation of new materials requirements for drug delivery. Discussion: A full evaluation on the toxicity, biodistribution and immunology of these materials are envisaged to boost their application potentials. Results: VLPs can easily offer the requirements that are needed for a drug delivery, such as biocompatibility, solubility in water, and high uptake efficiency.}, Keywords = {virus, virus like particle, nano-carrier, drug delivery.}, volume = {5}, Number = {17}, pages = {57-67}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-322-en.html}, eprint = {http://ncmbjpiau.ir/article-1-322-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {BagherpourDoun, Kazem and akbarzadeh, Azim and HalalKhor, Sohrab and EbrahimShahmabadi, Hasan and Alavi, Ebrahim and Mortazavi, Mehri and Saffari, Zahra and Farahnak, Maryam}, title = {In vitro evaluation of effect of sodium chloride on stability of cytotoxicity property cisplatinum in dimethyl sulfoxide solvent on G-292 cell line}, abstract ={Introduction: Cisplatin (Cispt) is an anti-cancer drug with a low level of solubility. One of Cispt`s solvents is dimethyl sulfoxide (DMSO) which can be substituted with chlorine of drug as Cispt`s solvent. Using this solvent is impossible in biological studies due to intense reduction in activity. On the other hand, it is specified that Cispt`s stability is increased in aqueous media by increasing sodium chloride (NaCl) concentration up to 0.9%. Consequently, we intended to study the effect of DMSO on cytotoxicity of Cispt in the presence of sodium. Materials and Methods: G-292 cells and MTT assay was used to study cytotoxicity of different compounds in culture media. FTIR was employed to investigate chemical structure of Cispt and Cispt dissolved in DMSO. Results: cytotoxicity in dilutions of 300 and 9 (p<0.01) of Cispt in Cispt+NaCl+DMSO formulation was equal to 78 and 7%. These numbers were estimated 79 and 18% for Cispt+ DMSO formulation. Studying chemical structure of Cispt and Cispt dissolved in DMSO showed that sodium chloride cannot inhibit inactivating effect of DMSO on Cispt and effect of this solvent on Cispt is independent from presence of sodium chloride. Conclusion: This study showed that effect of solvent on Cispt is independent from presence of sodium chloride. Our findings suggest more studies to use this solvent for Cispt.}, Keywords = {Dimethyl sulfoxide, Sodium chloride, Cisplatin, Cytotoxicity}, volume = {5}, Number = {17}, pages = {68-73}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-275-en.html}, eprint = {http://ncmbjpiau.ir/article-1-275-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {HasanpourZaferani, Zahra and Bayat, Mansour and RoudbarMohammadi, Shahl}, title = {Evaluating the Adherence of Fluconazole Resistant Candida albicans Species in comparison with Candida glabrata Species on Vagina and Intestine Cell Lines}, abstract ={Aim and Background: Vulvovaginal candidiasis is one of the most common female genital tract infections that is caused by overgrowth Candida species, especially Candida albicans and sometimes becomes chronic and recurrent form. Candida glabrata is the second or third leading cause of candidiasis after C. albicans. Following the widespread and increased use of immunosuppressive therapy along with broad-spectrum antimycotic therapy, the frequency of mucosal and systemic infections caused by C. glabrata has increased significantly. Fluconazole is one of the most common drugs used for treatment of this type of candidiasis but resistant is also seen. The first step in infection is adherence to epithelial cells. This study was designed to evaluate the adherence of fluconasole sensitive or resistant Candida albicans and Candida glabrata to Vagina and Intestine cell lines. Material and Methods: In this study, 160 vaginal swabs were collected from women with vaginal candidiasis. Then samples were cultured on Sabaouraud Dextros Agar and Chromagar for morphologic analysis. For final identification of Candida species the PCR-RFLP was done and C. albicans and C. glabrata were selected for our study. Hella and HT29 cell line cultured in DMEM medium supplemented with FBS. The yeast cells were adjusted to 1×106 cell and then cultured with definite number of each cell line separately in 96- well micro plate and incubated in 37°C for 24 hours. After this period, the number of adherent and non-adherent cells was counted by culture on Sabaouraud Dextros Agar and colony count assay. Result: According to our result, the clinical isolates are resistant to fluconazole and C. glabrata isolates had more ability to adhere to vaginal epithelial cells compared with C. albicans but, in contrast, C. albicans had more ability to adhere to intestinal epithelial cells rather than C. glabrata. Conclusion: This research showed that C. glabrata had more ability to adhere to vaginal cells. As regards, there is little information about the reaction between C. glabrata and innate immune system, Therefore, recognition of the reactions and factors that mediate adherence to host epithelial surfaces has a particular importance for finding new aspects in the pathogenesis and a more suitable treatment for the disease.}, Keywords = {Vulvovaginal candidiasis, RFLP-PCR, vaginal and intestinal cell lines}, volume = {5}, Number = {17}, pages = {74-80}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-535-en.html}, eprint = {http://ncmbjpiau.ir/article-1-535-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Bagheri, Gita and AbedinDorkoosh, Farid and Vasheghani-Farahani, Ebrahim and Ardjmand, Mehdi}, title = {Design of controlled release of Olanzapine by using Glycerol Monooleate in Sol-gel Systems}, abstract ={Backgrounds and Objectives: This research is to develop new systems for drug release systems based on sol - gel systems. The objective of this study is to develop controlled release formulation of water insoluble Olanzapine (OZ), using Glycerol monooleate (GMO) and Polyethylene glycol (PEG 300). Materials and Methods: A Box- Behnken response surface methodology was applied to design gel system with 3 factors with an initial drug containing, weight ratio of GMO/water (w/w) and weight ratio of PEG 300/GMO (w/w). Results: Therefore, in this study Design-Expert® software with Box- Behnken response surface methodology has been used specifically to determine the relationships between variables and responses. Discussion: According to the results of experiments, a quadratic model as an appropriate equation has been selected to fit the percentage of loading. Percentage of release at 12th and 168th hr a cubic model is selected. Kinetic release profile of OZ was investigated with several models, from which Higuchi model showed the best fitting and highest correlation. Conclusion: Optimization of gel system was carried out based on statistical concept of experimental design. Validation test was carried out under optimum conditions of the parameters predicted by the polynomial model.}, Keywords = {Olanzapine, Glycerol Monooleate, Cubic phase, In vitro release, Response surface methodology Box- Behnken.}, volume = {5}, Number = {17}, pages = {81-88}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-372-en.html}, eprint = {http://ncmbjpiau.ir/article-1-372-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Behrouznasab, Kasra and Razavi, Mohammad Reza and Fathalian, Fatollah and Seirafi, Hassan and Nejadsattari, Taher}, title = {Diagnosis of skin infections caused by atypical Mycobacterium using PCR method}, abstract ={Aim and Background: Granulomatous skin infections probably originated from an environment and through skin wounds, scratching, trauma, surgery into the inoculated skin, and abscess pus appears and sometimes is in form of Ulcerativa. Infection is limited to the skin and in case of immunosuppression would transmit and spread in form of infection. PCR is a rapid sensitive, specific and accurate method for diagnosis of bacterial infections and pathogens in the shortest time. Materials and Methods: In this study atypical Mycobacterium granulomatous skin infections diagnosed by PCR, using specific primers design for Mycobacterium genes (16srRNA) and confirmation by sequencing methods (sequencing of nucleotides of) atypical mycobacterium and comparing with genome sequencing of 16srRAN genes atypical mycobacterium in Gene Bank and application of this technique in the production of atypical mycobacterium skin infections diagnostic kits. Result: Among 58 studied paraffin blocks samples of skin tissue, 18 samples were in the range of positive control and established bands showing positive PCR results. Out of 18 positive samples of Mycobacterium granulomatous skin lesions, 83% were men and 17% women. In 13 cases (72%) there were occupational causes and in (28%) lack of occupational causes. 66% of mycobacterium granulomatous skin infections (NTM) were in adults over age 40 years. Conclusion: PCR is a rapid, specific, sensitive and accurate method for the diagnosis of bacterial infections and pathogens in the shortest time. This method can recognize a few atypical Mycobacterium genomes in samples, which is the superior advantage of this method, compared to conventional pathological methods such as culture and differential biochemical experiments.}, Keywords = {Mycobacterial}, volume = {5}, Number = {17}, pages = {89-95}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-402-en.html}, eprint = {http://ncmbjpiau.ir/article-1-402-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Habibnia, Shadi and Rasoulinasab, Masoumeh and Heidarieh, Parvin and FatahiBafghi, Mehdi}, title = {New method for DNA extraction of the genus Nocardia}, abstract ={Aim and Background: Nocardia spp are Gram-positive, partially acid-fast causing various infections. Due to the complex structure of the cell wall of the microorganism that is similar to mycobacteria, DNA extraction from this bacterium is different from other bacteria. The boiling method with STET buffer solution was used for DNA extraction of Nocardia. Materials and Methods: Nocardia colonies were suspended in 200 µl STET buffer and was boiled for 30 minutes. Suspension was centrifuged and transferred to another micro tube and Ethanol %95 was added and saved at -20 °C for 30 minutes. After this stage, sample was centrifuged and the supernatant was discarded. In the first stage, distilled water was added and stored at -20 °C for molecular works. To confirm the presence and quality of the DNA extract, the electrophoresis on a %1 agarose gel was carried out and PCR reaction 16S rRNA gene was used. Results: DNA was extracted on agarose gel and the purity and quality was appropriate. 16S rRNA gene PCR was then performed and observed to be 1500 bp.}, Keywords = {Nocardia, DNA extraction, STET buffer, 16S rRNA gen}, volume = {5}, Number = {17}, pages = {96-99}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-350-en.html}, eprint = {http://ncmbjpiau.ir/article-1-350-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Ghorbani, Masou}, title = {A Review of Type 2 Diabetes and Obesity}, abstract ={Here in this paper, we describe the relationship between type 2 diabetes and obesity including different types of obesity and its genetic predisposition. Several physiological conditions that are capable of changing the level of expression of numerous genes involved in metabolic pathways, energy production, and regulating body weight, are created due to the expansion of our modern society and abundance of caloric foods. It appears that a variety of genes could participate in adaptation of human body to its revolutionary limitations.  Therefore, due to the importance of obesity in relation to metabolic disorders by increasing the visceral adipose tissue, induction of higher level of free fatty acids in the blood and insulin resistance, we conclude that there is a close relationship between the obesity and type 2 diabetes and further genetic analysis in juveniles and at early ages could give a better insight of the occurrence of obesity and its treatment.}, Keywords = {Type 2 Diabetes, Obesity, Metabolism}, volume = {5}, Number = {18}, pages = {9-14}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-618-en.html}, eprint = {http://ncmbjpiau.ir/article-1-618-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Tebyanian, Hamid and YousefiSabeteMozhdeheh, Behjat}, title = {Evaluation and comparison of the effect of ORS and pH stress on the biofilm formation and planktonic condition of E.coli O157: H7}, abstract ={Aims and Background: E.coli O157:H7 is a bacterium from the family Enterobacteriaceae which are Gram negative cocobacilli, causing gastrointestinal diseases such as Diarrhea, Hemorrhagic colitis and hemolytic uremia. The aim of this study was to examine E.coli O157:H7 in plankton and biofilm status under stressed conditions of pH and ORS powder at 37 0C and then comparing their shape both in microscopic (bacterial shape) and macroscopic levels (bacterial colonies). Materials and Methods: Bacterial strains of E.coli O157:H7 were purchased from Razi Vaccine and Serum Manufacturing Co. with No. (RITCC) 2323. The bacteria were placed in liquid Nutrient Broth culture media and then transferred to Nutrient Agar culture media into plates to examine colonial shape. Next, colonial and bacterial shapes were examined by gram dying. Counting the number of live bacteria under stressed conditions was done by poor plate method and diluting preparation. In order to determine the power of biofilm formation under stressed, microtitration plate was used and read by Elisa Reader. Results: Bacterial shape is affected by stressed conditions. Under stressed conditions, colonial morphology and bacterial shape significantly change and the shape tends to transform to Cocci and bacilli in order to survive longer. Survival of bacteria under stressed conditions is more in biofilm state than Plankton. Conclusion: these changes have a direct relationship with the survival of most bacteria in the digestive tract preventing the natural effect of gut antibacterial compounds on bacteria and the resistance of bacteria in biofilm is more than plankton.}, Keywords = {Planktonic, Biofilm, Escherichia coli O157:H7}, volume = {5}, Number = {18}, pages = {15-22}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-622-en.html}, eprint = {http://ncmbjpiau.ir/article-1-622-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Fakhroleslam, Mohammad Reza and Jafari, Parvaneh and hosseini, Davoud and Mohajerani, Hamid Reza and Banasaz, Ali and TajabadiEbrahimi, Maryam}, title = {Connection, evaluation and detoxification of aflatoxin M1 in milk by Lactobacillus casei strain TD2, native probiotics in various physicochemical conditions}, abstract ={Aim and Background: Contamination of foods such as milk and dairy products even with low level of Aflatoxins has side effects on human and animal health. Based on the International Agency for Research on Cancer (IARC), Aflatoxins are classified as human carcinogenic factors. Several microorganisms have been reported to have the ability to bind or destruct Aflatoxins in food and feed. Most of the scientists believe that detoxification is accomplished by physical binding between the cell wall and toxin.the aim of this study was the investigation of Aflatoxin M1 detoxification ability of Lactobacillus casei strain TD2, native Iranian probiotic isolated from Tarkhineh by Tajabady and colleagues. Material and Methods: several samples of milk were collected from different regions of central province and the levels of their aflatoxin M1 was quantified with ELISA kit (Euro clone). The strain was subcultured in MRS Broth and 50 µl of it with 1×109 CFU/ml was inoculated in microfuges tubes containing 950 microliters of milk with aflatoxin M1 concentration about 100 ppb. After 4 hours incubation at 37°C and pH 7, the decrease in aflatoxin level was calculated. Then the detoxification abilities were determined in the same condition with different treatment such as heat (autoclaving), acid (hydrochloric acid), enzymes (lysozyme and protease) and physical (sound) treatment. By this way the effects of several factors on detoxification were examined. Results: Our result showed that this bacterium can bind to Aflatoxin M1 (% 55.35 ± 0.7568), but the destruction of it’s cell wall cause revealing of unavailable sites in cell wall and membrane components and enhance detoxification ability. Treatments with Lysozyme and hydrochloric acid 2 M resulted in higher detoxification levels, 60.03 ± 0.5144 and % 63.61 ± 0.5886, respectively. Conclusion: The results of this study showed that ther is no differences between living and non-living cells of Lactobacillus casei TD2 in Aflatoxin M1 removal. In another word, living of the cells is not essential for detoxification. Acidic treatment of the cells was resulted in the highest level of toxin removal between all of the treatment. A change in the cell wall structure is the possible reason for this observation. The percent of bacteria remove by acid treatment showing higher aflatoxin M1 may be due to changes made in the wall structure. In general it can be concluded that the strain TD2 with %55.35 removal of Aflatoxin M1, can be used in biological removal of toxin in different materials.}, Keywords = {milk, Lactobacillus casei (TD2), Aflatoxin M1, ELISA}, volume = {5}, Number = {18}, pages = {23-28}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-621-en.html}, eprint = {http://ncmbjpiau.ir/article-1-621-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Erfani, Mostafa and Shirmardi, Seyed Pezhm}, title = {The synthesis of labeled colchicine and the study of its biological distribution}, abstract ={Aim and Background: Colchicine alkaloid is originally extracted from plants of the Colchicum automnale L. due to its anti- mitotic effects in the treatment of gout and Mediterranean fever. Materials and Methods: Colchicine has been labeled by HYNIC (hydrazino nicotinic acid) chelator to 99mTc. After labeling by HYNIC-99mTc, it is injected via intravenous route to mice and the biodistribution of this radiopharmaceutical is investigated. Results: The results showed a significant efficiency for the combination. It is seen a relatively rapid removal of compound from the blood with a rapid accumulation in kidney and liver. This compound accumulates in the liver and secreted into the intestine immediately so it is observed in the small and large intestines. These organs were identified as the target organs. Conclusion: According to the results, this labeled compound can be used as an agent to study the function and response to therapy by Colchicine especially in the organs which have no secretive accumulation.}, Keywords = {Colchicum automnale L, Colchicine, 99mTc, biodistribution}, volume = {5}, Number = {18}, pages = {29-34}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-627-en.html}, eprint = {http://ncmbjpiau.ir/article-1-627-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Toloei, Saba and KoohiMoftakhariEsfahani, Maedeh and Movahedi, Fatemeh and Alavi, Seyed Ebrahim and Akbarzadeh, Azim}, title = {Synthesis and characterization of bacterial cellulose by Acetobacter xylinum ATCC 10245}, abstract ={Aims and Background back: Bacterial cellulose has properties such as higher elasticity, strength and biocompatibility and produced fibers are 100 times smaller than natural cellulose. It is applicable as three dimensional extracellular matrices for holding cells, controlling tissue structure and regulating cell activities. Materials and Methods: Bacterial cellulose was produced by Acetobacter xylinum ATCC 10245. Then Cellulose scaffold was synthesized and characterized. Microstructures, formed bonds and crystalline structure were analyzed by scanning electron microscope (SEM), Fourier-transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD), respectively. Results: The fibers were synthesized with diameter of about 30 nm to 360 nm. Different pore sizes of more than one micron were observed. Fiber length was in micron scale. Thus microbial cellulose was quite finer in comparison with natural cellulose. Furthermore, the amount of synthesized bacterial cellulose was in direct relation with area of medium in contact with air. Conclusion: Considering the results and especially existence of micron scale pores, synthesized bacterial cellulose was quite applicable in tissue engineering.}, Keywords = {Bacterial cellulose, Acetobacter xylinum, Synthesize, characterization}, volume = {5}, Number = {18}, pages = {35-42}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-624-en.html}, eprint = {http://ncmbjpiau.ir/article-1-624-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {BakhshiKhaniki, Gholamreza and Fakhri, Maryam}, title = {The effect of chilling and chemical treatment on seed dormancy of Capparisspinosa L}, abstract ={Aim and Background: Capparisspinosa L. is considered as one of the most significant plant species in the capparaceae. This plant is one of the most important medicinal plants and has a great ability to stabilize sand dune. Seeds of this plant have germination problems. The aim of this research is to determine germination percentage of Capparisspinosa according to different duration of chilling (0, 10, 20, 30, 40, 50, 60, 70 and 80 days) and temperature (1-4, 7-9 c). Materials and Methods: Cold stratification procedures under in-vitro condition were applied for eliminating seed dormancy to find the most suitable germination conditions because the presence of seed dormancy causes difficulties in seedling production. In order to break seed dormancy, this experiment has been in the base of completely Randomized Design in 3 replications Results: Data analysis showed that the highest germination percentage (69.91%) was obtained in seeds that were in concentrated sulfuric acid for 30 minutes before being in cold stratified condition for 60 days, in1-4c, the lowest germination percentage (2.86%) was determined in control seeds Conclusions: Based on these results, the application of chilling and chemical treatment on seed dormancy of Capparisspinosa is very important and effective on defecting seed dormancy, which can affect the economical importance of this species}, Keywords = {Capparisspinosa, germination percentage, dormancy, chilling}, volume = {5}, Number = {18}, pages = {43-48}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-625-en.html}, eprint = {http://ncmbjpiau.ir/article-1-625-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Mohammadpour, Mahdi}, title = {Isolation and identification of Mycoplasma agalactiae by culture and polymerase chain reaction (PCR) from milk of sheep in Masal, Guilan}, abstract ={Aim and Background: Contagious agalactiae (C.A) is a serious disease syndrome of sheep and goat characterized by mastitis and subsequent failure of milk production, arthritis, abortion and kerato-conjunctivitis. Mycoplasma agalactiae (M. agalactiae) is the main cause of the disease in sheep and goat. The aim of this study was isolation and identification of M. agalactiae with culture and polymerase chain reaction (PCR) assay from milk of sheep in Masal, Iran Material and Methods: A total of 25 samples were collected from milk secretion of sheep. All samples were cultured in PPLO broth supplemented for M. agalactiae isolation. The bacteria DNAs were extracted by High Pure PCR Template Preparation kit and the PCR assay was applied for the detection of Mycoplasma genus in 270bp fragment in 16S rRNA gene and M. agalactiae in 375bp fragment in lipoprotein gene from culture as well as clinical samples. Results: Of the 25 samples, 9 (36%) yielded one of the potentially pathogenic Mycoplasmas evaluated for using PPLO agar culture diagnostic method, and 17 (68%) yielded one of the potentially pathogenic Mycoplasmas evaluated for using Mycoplasma genus PCR as diagnostic method, and 5 (29/4%) of the samples yielded M. agalactiae by using M. agalactiae PCR as diagnostic method. Of the 25 samples, 8 samples were culture positive and PCR positive, 7 samples were culture negative and PCR negative whereas 9 samples were culture negative and PCR positive and only one sample was culture positive and PCR negative. Conclusion: The result showed that PCR can be used as an alternative method instead of culturing to identify M. agalactiae. M. agalactiae was detected for the first time from milk of sheep in Masal, Iran and it was not the main factor of C.A there.}, Keywords = {Mycoplasma agalactiae, Contagious Agalactiae, Culture, PCR, Sheep}, volume = {5}, Number = {18}, pages = {49-54}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-626-en.html}, eprint = {http://ncmbjpiau.ir/article-1-626-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Soltanmoradi, Raheleh and Nazemi, Ali and Hoseinidoost, Seyed Reza and Khodaparast, Ali Asghar and delbisheh, Momeneh}, title = {Molecular Screening of Serratia Marcescens Producing Serratiopeptidase Enzyme and Prodigiosin Pigment from Paddy Field Waters in West Mazandaran}, abstract ={Aims and Background: Serratiopeptidase is a proteolyitic enzyme produced from Serratia Sp. and is used as a medicine against chronic inflammatory diseases. Prodigiosin is also produced by Serratia marcescens and is used in medicine production. This paper aims to separate and discover a novel strain (which is able to produce Serratiopeptidase and Prodigiosin) of Serratia marcescens from paddy field waters located in western Mazandaran. Materials and Methods: In this study, 50 water samples were collected under standard conditions from paddy fields. As many as 40 bacteria samples were separated among which 10 bacteria samples had protease activity and Prodigiosin. They were separated using biochemical tests. Then, the strain was discovered using 16SrRNA. The molecular weight of the enzyme was measured using protein deposition and SDS-PAGE. Then, Prodigiosin production capability was experimented using ultraviolet spectrophotometry at wavelength 535 nm under 25, 28, and 37 0C in the following culturing environments: nutrient broth, MacConkey broth, Luria-Bertani Broth, and skim milk agar. Results: Only one isolate was discovered. Its molecular weight was about 52 kda. Pigment production was maximized in skim milk agar at 28 0C. The bacteria were able to produce the pigment even at 37 0C. Conclusion: Considering the importance of Serratiopeptidase and Prodigiosin, we recommend to optimize the conditions to produce them in a larger amount.  The molecular weight of Serratiopeptidase was 52 kda. This result confirms the observations of Mohankumar et. al. 2011. Pigment production was entirely stopped under 37˚C. This result confirms the observations of Pryce and Terry 2000.}, Keywords = {Serratia marcescens, 16SrRNA, Serratiopeptidase, Prodigiosin, spectrophotometry}, volume = {5}, Number = {18}, pages = {55-62}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-628-en.html}, eprint = {http://ncmbjpiau.ir/article-1-628-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Sattari, Samaneh and KhesaliAghtaei, Hoda and Vahabzadeh, Farzaneh}, title = {Esterification of methanol and oleic acid using loofa-immobilized Rhizopus oryzae}, abstract ={Aims and Background: Biodiesel as a mixture of alkyl esters have reasonable compatibility with environment. Rhizopus oryzae with producing intracellular lipase (as whole cell) could catalyze the transesterification process. Effect of immobilization on enzyme activities at different cultivation times was also evaluated. Materials and Methods: Rhizopus oryzae free cells or immobilized on loofa support particles were used as biocatalyst. Titration method was used for measuring the hydrolytic activity and determination of free fatty acid content was carried out with colorimetric method. Results: Results show that cell immobilization results in increasing intracellular lipase activity. Furthermore, the best lipase activity was achieved using the 48-h cultivated biocatalyst. The experimental observations reveal that methyl oleate yield increase with step-wise addition of methanol. Conclusion: Cell immobilization enhances intracellular lipase production. Loofa-immobilized Rhizopus oryzae is an appropriate catalyst for ester synthesis.}, Keywords = {Methyl oleate, Lipase, Rhizopus oryzae, Loofa, Biodiesel.}, volume = {5}, Number = {18}, pages = {63-68}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-629-en.html}, eprint = {http://ncmbjpiau.ir/article-1-629-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Hasanzade, Zahra and Amoabedyni, Ghassem and Seifkordy, A.Akbar and Vaziri, Ali}, title = {Synthesis of the Bio-functionalized magnetic nanoparticles with starch}, abstract ={Aim and Background: In the last years, a variety of biopolymers have been investigated as soft matrices to accommodate magnetic nanoparticles. Polymer encapsulation provides the surface functionalization and protection of the magnetic core from environmental perturbation. Furthermore, polymer coating could enhance the compatibility between nanoparticles and aqueous medium, prevent particles from oxidation, reduce toxicity, and extend storage life. Materials and Methods: In this study, new magnetic nanoparticles were synthesized by the co-precipitation in the presence of starch. The obtained nanoparticles were characterized by transmission electron microscopy (TEM), Fourier transform infrared (FT-IR) spectroscopy, and X-ray diffraction (XRD). Results: According to the analysis carried out, particles less than 40 nm were obtained and starch-coated magnetite nanoparticles were well synthesized. Conclusion: This work provides a simple and fast method for obtaining starch-coated nanoparticles.}, Keywords = {Bio-functionalization, Magnetic nanoparticles, Co-precipitation, Biopolymers, Starch}, volume = {5}, Number = {18}, pages = {69-74}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-630-en.html}, eprint = {http://ncmbjpiau.ir/article-1-630-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Farhadzadeh, Nasrin and Hosseini, Seyed Davood and Porbabai, Ahmad Ali}, title = {MOLECULAR DETECTION AND IDENTIFICATION OF GRAM-POSITIVE COCCI, STREPTOCOCCOSIS AGENT ISOLATED FROM RAINBOW TROUTIN MARKAZI PROVINCE}, abstract ={Aims and Background: Streptococcosis is one of the most important diseases of the farmed fishes specially, rainbow trout, causing the death of many fishes and also leading to heavy damages on aquaculture centers. This research deals with the exact recognition and determination of Gram-positive cocci isolates (Streptococcosis agent), using PCR and sequencing analysis. Materials and Methods: the sampling was performed during spring and summer 2011 from 12 farms of Markazi province and 10 fish were selected with death-sick or clinical signs from each farm. Firstly the samples were cultivated in blood agar and incubated for 72 hours in 37˚C, then the conventional microbiology experiments were conducted on the suspected samples to confirm Streptococcosis disease. Also antibiogram test was carried out using disk diffusion method to determine the effective antibiotic type on known bacteria. All the positive microbial samples were subjected to PCR and sequencing experiments. Results: In this research, 12 isolates of Gram-positive cocci were separated from kidney, brain, liver and spleen of 120 rainbow trout patients by microbiological and some biochemical tests. All positive microbial samples in PCR test (based on 16SrRNA gene) were recognized positive and determined as Lactococcus garvieae based on sequencing analysis. The resistance of gram-positive cocci to colistin, lincomycin, cloxacillin, streptomycin and nalidixic acid were shown on antibiograms disk diffusion experiment. Conclusion: Lactococcus garvieae is major gram-positive cocci in infected fish farms in Markazi province.}, Keywords = {PCR, rainbow trout, Streptococcosis, 16SrRNA}, volume = {5}, Number = {18}, pages = {75-82}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-631-en.html}, eprint = {http://ncmbjpiau.ir/article-1-631-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Salehi, Mitra and Tabibzadeh, Mahnaz and Fallahian, Mohammad Rez}, title = {Prevalence of qnr gene in clinical Acinetobacter isolates}, abstract ={Aims and Background: This study investigated the prevalence of qnr genes and resistance to fluoroquinolone antibiotics among clinical isolates of Acinetobacter in Tehran. Materials and Methods: Prevalence of qnr genes among Acinetobacter isolates were determined by PCR technique. Also by the use of antibiogram method, susceptibility of isolates against the fluoroquinolone family of antibiotics, piperacillin-tazobactam, and colistin were examined. Results: Evaluation of PCR in the 50 qnr-positive Acinetobacter isolates showed that 74% of them had qnrA and 22% had qnrB. Almost all of isolates were resistant to the fluoroquinolones. Conclusion: This study showed a high prevalence of qnr genes and a higher prevalence of genotype qnrA than qnrB.}, Keywords = {Acinetobacter, gene qnr, genotype qnrA, genotype qnrB}, volume = {5}, Number = {18}, pages = {83-88}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-632-en.html}, eprint = {http://ncmbjpiau.ir/article-1-632-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Fallahzadeh, Ramin and Fallahmehrabadi, Jalil and mirzaei, Amir and Ghafari, Mohammad D}, title = {Cloning and expression of Deinococcus radiodurans drRRA gene in E. coli Origami strain}, abstract ={Aim and Background: Deinococcus radiodurans is one of the most radio resistant microorganisms able to survive in an extreme environment. Available data show that multiple DNA repair and antioxidant proteins are involved in radio resistance. DrRRA is a novel response regulator essential for the radio resistant of Deinococcus radiodurans. The aim of this study was cloning and expression of drRRA gene in E. coli system. Material and methods: The drRRA gene of Deinococcus radiodurans was synthesized in pGEM-B1 vector and the synthetic gene was subcloned to pET21a expression vector. Subcloning of the gene was verified by double digestion and sequencing. The cloned gene was expressed in E. coli (Origami strain). Expression of recombinant DrRRA (rDrRRA) was confirmed by polyacrylamide gel electrophoresis and Western blotting. Results: The chimeric pET21a plasmid containing the C-terminal fusion of His-tag with DrRRA was successfully constructed. Recombinant DrRRA was intracellularly produced by transformed E. coli. Conclusion: The results of this study indicated that subcloning and expression of drRRA gene in E. coli is feasible. In addition, the expression level of protein was suitable and it should be analyzed in prokaryotic and eukaryotic systems.}, Keywords = {Radiation resistant, drRRA gene, Deinococcus radiodurans}, volume = {5}, Number = {18}, pages = {94-89}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-623-en.html}, eprint = {http://ncmbjpiau.ir/article-1-623-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Eslami, Gita and Samadi, Roghayeh and Taherpor, Arezu}, title = {Frequency of Listeria monocytogenes prfA, actA , inlB genes among infertile women referred to Medical center of University by PCR Method in 2013}, abstract ={Aim and Background: Recently the spontaneous abortion has been thea matter of attention by researchers. Therefore, bacteria such as Listeria monocytogenes, Chlamydia trachomatis, Mycoplasmas and Streptococcus agalactia are important agents of this phenomenon. When they are not recognized in due time , we found spontaneous abortion and increase of antibiotics resistance. The aim of this study was to assess the extent of L. monocytogenes in causing human spontaneous abortions by isolation methods and PCR analysis for the presence of actA, PrfA and InlB genes. Material and Methods: In this descriptive study, vaginal swabs were collected from a total of 96 women with spontaneous abortions who referred to Tehran`s hospitals. The samples were transported in to TSBYE broth. Then, the samples were enriched at 4 ͦ C for 1 month. Total DNA of L. monocytogenes was extracted by Fermentase kit. PCR test is performed on all samples and detected the actA, PrfA and InlB genes of L. monocytogenes. Results : In PCR technique, actA, PrfA and InlB gene were detected 12.5%, 9.3% and 2% respectively from 96 patients with spontaneous abortions. Conclusions: The occurrence of pathogenic L. monocytogenes in cases of spontaneous abortions was 12.5%. It seems that the actA, PrfA and InlB genes have an important role as essential virulence determinant in pathogenic L. monocytogenes.}, Keywords = {spontaneous abortions, Listeria monocytogenes, PCR}, volume = {5}, Number = {18}, pages = {95-99}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-633-en.html}, eprint = {http://ncmbjpiau.ir/article-1-633-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Alaee, Azade and Hamedi, Javad and Mohammadipanah, Fatemeh and MohammadiMotamed, Saeed and Rezayat, Seyed Mehdi}, title = {Evaluation of antioxidant and cytotoxic activity of local actinomycetes}, abstract ={Aim and Background: Among more than 23,000 microbial secondary metabolites discovered, 42% of them are separated from actinobacteria. These secondary metabolites have wide range of biological activities such as antibacterial, antifungal, antiparasitic, antiviral, antitumor, immunosuppressive, insecticide, antiinflammatory, antioxidant and other activities. In the past few years, numerous studies have been conducted on these biological activities in the country, but no report has been published about the antioxidant activity of local actinomycetes resources. The purpose of this research is to achieve natural antioxidants with lower physiological hazards associated with local microbial resources. Materials and Methods: Fifty strains of native actinomycetes preserved at the University of Tehran Microorganism collection were selected randomly and after sporolation and prefermentation and fermentation, bacterial extracts were prepared. At first, antioxidant activity of the extracts was evaluated by DPPH method. These results were evaluated with synthetic antioxidants including ascorbic acid and BHA. Also cytotoxic activity of the extracts was measured on Artemia. Elected strains were recognized with molocular method. Results: According to the results, 10% of extracts have no antioxidant activity, 20% of the extracts have high antioxidant activity (IC50<200), 32% of extracts with medium antioxidant activity (200<ic50 </ic50}, Keywords = {actinomycetes, DPPH, Artemia test, antioxidant compounds}, volume = {5}, Number = {19}, pages = {9-18}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-655-en.html}, eprint = {http://ncmbjpiau.ir/article-1-655-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Khatami, Hamid reza and fallahzadeh, Ramin and fallahmehrabadi, Jalil and Ghafari, Mohammad D}, title = {Expression assessment of recombinant human granulocyte colony stimulating factor (hG-CSF) in E. coli (Origami / BL21)}, abstract ={Aims and Background: Human granulocyte colony stimulating factor (hG-CSF) is a hematopoietic cytokine that stimulates activation of mature blood cells. Nowadays, recombinant hG-CSF is successfully used for the treatment of neutropenia in patients suffering from cancer of myeloid suppressor, bone marrow transplant, acute myeloid leukemia and acute/severe neutropenia. The aim of this study was the increased expression of hG-CSF and production of the soluble protein with disulfide bonds by E.coli strains (Origami and BL21). Materials and Methods: The synthesized hG-CSF gene (in pGEM vector) was subcloned into pET23a expression vector and then the recombinant plasmid was transformed into Origami and BL21 hosts. Afterwards, expression levels were compared in two hosts. Results: Enzymatic digestion results showed the accuracy of the cloning procedures in vector pET23a. Also under the same conditions and after induction of different hosts with IPTG, best hG-CSF protein expression was observed in strain Origami E.coli.Conclusion: The results showed that E.coli (Origami) is appropriate strain for the expression of human G-CSF and this strain can be used as suitable host for the expression of this protein in higher scale.}, Keywords = {granulocyte colony stimulating factor (hG-CSF), Recombinant Protein, optimization of codon, E.coli Expression strains}, volume = {5}, Number = {19}, pages = {19-23}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-657-en.html}, eprint = {http://ncmbjpiau.ir/article-1-657-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {KhesaliAghtaei, Hoda and Sattari, Samaneh and Vahabzadeh, Farzaneh}, title = {Immobilization of Rhizopus oryzae on loofa sponge affects interacellularly produced lipases}, abstract ={Aim and background: lipase is a carboxylic ester hydrolase enzyme (EC.3.1.1.3), which acts on triglycerides to release fatty acids, monoglycerides, diglycerides and glycerol. With respect to the biological process advantages against chemical process plus the ability of lipase to catalyze the revers reaction (i.e., esterification) a vast chapter on this enzyme scientific applications was started. The focusing point in this study was to determine the optimum cultivation time for free and immobilized form of Rhizopus oryzae with regard to producing membrane-bound lipase. With the usage of n-butanol and oleic acid in a non-polar environment (n-hexane as solvent) the esterification reaction was carried out while the effect of molecular sieves on the final yield was considered. Materials and methods: Rhizopus oryzae cells in the form of immobilized on cellulose support particles or free cells are used as a whole cell bicatalyst. Colorimetric method was used for measuring the esterification activity (i.e., determination of residual free fatty acid in reaction media). Results: Membrane-bound lipasees which are produced by both, immobilized and free form of the cell reach their maximum activity after 48 hour. Also the biocatalyst esterification activity was improved approximately 4-folds by the immobilization of fungal cell on loofa sponge. More than 96% efficiency was gained in synthesis of 1-butyl-oleate ester in the presence of 4 g molecular sieve. Conclusion: Immobilization could improve the membrane-bound lipase esterification activity. The lipase secretion to the culture media significantly affected by cultivation time. To reach a complete esterification reaction (approximately 100%), water adsorption with use of molecular sieve is an appropriate method.}, Keywords = {Rhizopus oryzae – loofa sponge– butylolaete – membrane-bound lipase – Molecular sieves}, volume = {5}, Number = {19}, pages = {24-31}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-658-en.html}, eprint = {http://ncmbjpiau.ir/article-1-658-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {EbrahimShahmabadi, Hasan and Rasouli, Rahimeh and Alavi, Seyed Ebrahim and KoohiMoftakhariEsfahani, Maedeh and akbarzadeh, Azim}, title = {Investigation of effective factors in preparation of polybutyl cyanoacrylate nanoparticles by emulsion polymerization}, abstract ={Aim and Background: In recent decades, drug carriers in nano scale have been focus of attentions. In last two decades, cyanoacrylate nanoparticles have been widely studied as drug nanocarriers. In this work, production of polybutyl cyanoacrylate (PBCA) nanoparticles by emulsion polymerization has been investigated. Material and Methods: polybutyl cyanoacrylate nanoparticles were produced by emulsion polymerization using 70 KDa dextran as stabilizer. Nanoparticles were described by Zeta sizer, scanning electron microscope and light microscope. Effect of polymerization factors on size and distribution of particles, was studied. Such factors include 70 KDa dextran, polysorbate-80, concentration of H+ ion, polymerization time, temperature and sonication. Results: This study indicates that a definite portion of stabilizer is essential for the production of nanoparticles. This concentration was recognized to be 2%. Effect of H+ ion was considerable as a sharp decrease was observed in pH 4. In comparison with room temperature, higher size and distribution of nanoparticles was gained in temperature of 50 °C. Effect of polysorbate-80 in concentration of 0.001% on nanoparticles was evaluated to be positive. Furthermore, increasing polymerization time from 5.5 to 18 hours resulted in synthesis of more appropriate nanoparticles. Discussion: The results illustrate that nanoparticles properties depend on various factors and manipulation of such factors can result in desirable properties. Conclusion: PBCA nanoparticles are under the influence of various factors and we can make an acceptable and desirable nanoparticle by their proper manipulation.}, Keywords = {Nano, poly butyl cyanoacrylate, Dextran, Emulsion Polymerization}, volume = {5}, Number = {19}, pages = {32-38}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-647-en.html}, eprint = {http://ncmbjpiau.ir/article-1-647-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Rouholamin, Sepideh and Zahedi, Bahman and Saei, Ali and NazarianFirouzabadi, Farh}, title = {COMPARISON OF THREE GENOMIC RNA EXTRACTION METHODS FROM POMEGRANATE PEEL}, abstract ={Aims and Background: RNA extraction with high quantity and quality is a fundamental requirement for molecular biology studies. Presence of carbohydrate, tannins, proteins and polyphenols compounds have created problem for extracted RNA from woody plant. Therefore, it is important to a suitable extraction method to produce a good quality RNA. Materials and Methods: Methods for RNA extraction were compared to choose the best method of RNA extraction from pomegranate (Punica granatum L.) peel. These methods included 1-Zarei et al 2- IHTB 3-CTAB–LiCl modified method. Results: Of the methods tested, Zarei et al method is the most appropriate method for plants with high phenolic compounds, especially pomegranate. Hence the need for the use of dangerous chemical material and extraction kits is not felt. Conclusion: At the end of experiment with attention to results and comparison between methods, Zarei et al method was selected as the best method for RNA extraction from fruit peel with high quantity, quality and purity. Maximum amount of RNA with high quality that extracted from Zarei et al method was 642 ng per 100 mg peel tissue.}, Keywords = {Spectrophotometry, RNA Extraction, Gel electrophoresis, Punica granatum, polyphenols compounds}, volume = {5}, Number = {19}, pages = {39-46}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-648-en.html}, eprint = {http://ncmbjpiau.ir/article-1-648-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Ahmadi, Leili and akbarzadeh, Azim and Chiani, Mohsen and MasoudSinaki, Jafar}, title = {To evaluate the effect of formulation of Nanoarchaeosomal 6-gingerol on the growth of breast cancer MCF-7 cell line}, abstract ={Aims and Background:Nanotechnology is used to design new formulations of drugs. Also, nanocarriers are utilized for drugs or biologically active substances to improve the therapeutic index. Chemotherapy effectiveness has direct proportion to increase the time of exposing cells near drug. Due to their small size the nanocarriers will then penetrate into the target tissue causing an efficient drug accumulation at the target position.6-gingerol which inhibited efficacy on cancer cell growth in this research preparation as formulation of nanoarchaeosomal PEGylated 6-gingerol and evaluate its impact on cancer cells MCF-7 cell line. Material and methods:First Halobacterium salinarum lipids were extracted and added 6–gingerol (dissolved in ethanol), Polyethylene glycol (PEG) 2000 and Twee n 80 to it and with the use of Avaporator extract the organic solvent, then were dissolved in buffer phosphate environment and Sonication. Their particle diameter measured with Zeta sizer device and finally evaluated the effect of the formulation cytotoxicity by MTT. Results:The diameter of the nanoarchaeosomal PEGylated 6-gingerol was equal to 290 nm. The encapsulation efficiency of 6–gingerol in nanoarchaeosomal PEGylated was also equivalent to 96/33 percent, respectively. The Cancer cells against the effect of this formulations showed difference in different doses. Conclusion:The dimensions of the nanoparticles and the encapsulation efficiency was suitable for targeted drug delivery, and nanoarchaeosoma PEGylated 6–ginger showed cytotoxicity effects on breast cancer cells MCF-7 cell line.}, Keywords = {nanocarriers, archaeosomal, 6-gingerol , MCF-7 cell line}, volume = {5}, Number = {19}, pages = {47-52}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-649-en.html}, eprint = {http://ncmbjpiau.ir/article-1-649-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Yazdansetad, Sajjad and Taheri, Raheleh and Ajoudanifar, Hatef}, title = {Production of microbial cellulose by native species of Pseudomonas luteola}, abstract ={Aims and Background: Cellulose is the most widely used biopolymer to produce paper, textiles and biomedical materials. One of the interesting methods to produce cellulose is microbial resource due to the shortage of cellulose original resources. On the other hand, the use of microbial cellulose as a pure polymer and absence of lignin and hemicellulose is preferred. The present study was aimed to isolate Pseudomonas luteola producing cellulose bacterium to produce cellulose and evaluate its production quality. Materials and Methods: Samples from different local vinegars, juices and fruits were cultured in Hestrin-Schramm agar medium. Pseudomonas luteola was isolated with biochemical tests and verified by 16S rRNA typing method. After cultivation of the isolate in Hestrin-Schramm broth, cellulose was produced in medium. The cellulose layer was treated by SDS and NaOH, then purified and dried. Finaly, the produced cellulose was verified by cellulase enzyme and scaning electron microscope (SEM). Results: In this study, the gram-negative organism Pseudomonas luteola was isolated and identified. Then, the isolate was cultured in Hestrin-Schramm broth and cellulose was produced after 6 days incubation at 30°C. The enzymatic digestion results were showed that the cellulose has been presented in culture medium. Scaning Electron Microscope (SEM) image was revealed that the produced cellulose by the isolate has fibrous and plexiform structure. Conclusion: This is the first report on cellulose production by native isolate of Pseudomonas luteola in Iran. The quality of produced cellulose by the organism is appropriate in comparison with the cellulose produced by standard strain, thus is presented to produce cellulose as a new resource.}, Keywords = {cellulose, Hestrin-Schramm, Pseudomonas luteola, Scanning Electron Microscope}, volume = {5}, Number = {19}, pages = {53-60}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-650-en.html}, eprint = {http://ncmbjpiau.ir/article-1-650-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Kiani, Parivash and Mahmoodi, Mohammad Mehdi}, title = {Production of biosurfactant by Lactobacillus for using in food industry as a substitution for synthetic emulsifiers}, abstract ={Aim and Background: Biosurfactants or Bioemulsifiers are surface tension-reducing compounds that are produced by many bacteria and fungi. Importance of these compounds in comparison with synthetic surfactants can be outlined as no toxicity, environmental friendly, activity in high temperature, pH and salinity. The purpose of this research was the isolation and identification of biosurfactant-producing Lactobacillus and evaluating the effectiveness of produced biosurfactant. Materials and Methods: In this study, sampling of dairy products and culturing in MRS medium was performed. The isolated strains with capability of production of biosurfactant were identified based on the biochemical and molecular methods. The optimum conditions of biosurfactant production were investigated and its emulsifier activity was studied in a food model system. Results: Altogether, nine strains of Lactobacillus isolated from the dairy products among them, Lactobacillus plantarum and Lactobacillus pentosus had the greatest capability for biosurfactant production. The extracted biosurfactants from both bacteria showed that they have good capability in emulsification process. Conclusion: The results of this research showed that it is possible to isolate strains of lactobacillus with appropriate capability of biosurfactant production and if the costs of production can be reduced, there is possibility of using such strains for production of biosurfactant in industrial scale as a substitution of synthetic emulsifiers in food industry.}, Keywords = {Lactobacillus, Biosurfactant, Surface active agents}, volume = {5}, Number = {19}, pages = {61-68}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-651-en.html}, eprint = {http://ncmbjpiau.ir/article-1-651-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {farooghi, Nasrin and maleki, Masoud and GhasemiGermi, Karimollah and Abdolmaleki, Arash}, title = {The study of toxicity and teratogenic effects of Nerium oleander leave’s extract on chicken embryo}, abstract ={Aims and Background: Nerium oleander is a medicinal plant with various biological activities such as antibacterial, antioxidant and cytotoxic effects. In the present work, methanolic extract of plant was used for the evaluation of toxicity and teratogenic effects on chicken embryo as an animal model. Materials and Methods: Methanolic extracts were prepared. Methanolic extract solutions in DMSO were injected in air sac of chicken eggs at concentrations of 5, 10, 20, 40, 60 and 80 μg/egg. Seventy two hours after incubation, eggs were placed at 37.7-38C° incubator. Results: On the day 19 after incubation, eggs were opened and shown that 78.4, 65, 56.7, 46.7, 31.66 and 21.66 percent of embryos were alive, Results showed that LD50 was at the concentration of 43.60μg/egg. Morphological and skeletal abnormalities in the treatment groups showed that club foot and beak deformity were occurred in morphology while caudal vertebrate deletions, unossification or uncalcification of caudal vertebrates were appeared in skeleton of the Chickens. Conclusion: The study of methanolic extract showed that, with increasing concentration, mortality increased. The extract in low concentrations had negligible toxicity and teratogenic effects which can be used as therapeutic aims.}, Keywords = {Fertile egg, Abnormality, Toxicity, Chick embryo, Nerium oleander}, volume = {5}, Number = {19}, pages = {69-76}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-652-en.html}, eprint = {http://ncmbjpiau.ir/article-1-652-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Nejatzadeh, Fatemeh}, title = {Effect of Biological and Chemical Nitrogen Fertilizers on Growth, Yield and Essential Oil Composition of Dill (Anethum graveolens L.)}, abstract ={Aims and Background: Dill (Anethum graveolens L.) is one of the most important medicinal plants with great applications in different medicinal industries. In present study, effects of biological and chemical nitrogen fertilizers were evaluated on growth, yield and essential oil composition of dill. Materials and Methods: This research was conducted under field condition in randomized complete block design with three replications and two factors. The treatments included Nitroxin biofertilizer in four levels (control, seed inoculation, topdress, seed inoculation and topdress), and chemical fertilizer was applied in three levels (0, 50,100 Kg/ha), combinations of biofertilizer and chemical fertilizer and control. Different characteristics such as plant height, number of umbel per plant, number of umbellet per umbel, number of grain per umbelet, 100 seed weight, grain yield, biologic yield and oil percentage were recorded. Results: According to the results, the highest height, biologic yield and grain yield components (except harvest index) obtained on biological fertilizer. Results showed the highest biologic yield and grain yield components, essential oil percent (except the number of umbel per plant, 100 seed weight and harvest index detected in chemical fertilizer). Results showed that the highest essential oil content was detected in biological fertilizer and chemical fertilizer. Identification of essential oil composition showed that content of carvone increased with the application of biofertilizers and chemical fertilizers. Conclusion: Application of biofertilizers enhanced yield and other plant criteria in this plant. Generally, it seems that using biofertilizers or combinations of biofertilizer and chemical fertilizer could improve dill performance in addition to reduction of environmental pollution.}, Keywords = {Anethum graveolens, biofertilizer, essential oil, Nitrogen, Nitroxin.}, volume = {5}, Number = {19}, pages = {77-84}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-653-en.html}, eprint = {http://ncmbjpiau.ir/article-1-653-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {shirmardi, Seyed Pezhman and Erfani, Mostafa and zare, Abbas and Sarzaeem, Ali}, title = {Preparation and investigation of ICD-85 biodistribution by 131I}, abstract ={Aims and Background: ICD-85 with weight of 10,000 to 30,000 Daltons is a triple peptide derived from Iranian brown snake venom, scorpion venom of Agkistrodon Halys and yellow Hemiscorpius lepturus. This compound inhibits cancer cells by inducing apoptosis. In this study, the preparation and labeling of this triple peptide has been done. Materials and Methods: At first, these triple peptides were isolated from snake and yellow scorpion venom by gel and high performance liquid chromatography. Labeling with iodine-131 was performed through chloramine-T method and radiochemical analysis involved sephadex G-25 method. The stability and biodistribution of radio peptide was checked in the presence of PBS and mice respectively. Results: The results of biodistribution showed that the labeled compound has about 15% accumulation in breast tumor and in other organs such as liver and kidney they have the highest radio peptide concentration. Tumor to blood ratio for radio peptide is more than 293 percent. Conclusion: The labeled ICD-85 by 131I can be an agent for diagnosis of breast cancer tumors.}, Keywords = {Iodine-131, ICD-85 triple peptide, labeling}, volume = {5}, Number = {19}, pages = {85-94}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-654-en.html}, eprint = {http://ncmbjpiau.ir/article-1-654-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Habibi, Ghader and abedini, Masumeh}, title = {Effects of postharvest silicon application on phenolics, antioxidant system and phenylalanine ammonia-lyase activity in plum (Prunus salicina Shablon) fruits}, abstract ={Aims and Background: In this study, the efficacy of silicon (Si) was determined in order to increase the pool of phenols and antioxidant activity in plum (Prunus salicina Shablon) fruits and, thereby, enhance postharvest plum fruit quality. Materials and methods: The fruits were randomly divided in two groups so that Si-treated group immersed for thirty minutes in solutions of 5 g/l Si and were air-dried at room temperature. The activities of catalase (CAT) and superoxide dismutase (SOD) were determined. Results: Postharvest silicon application had no effect on CAT activity in contrast, weight loss, total phenolics concentration, lipid peroxidation as well as phenylalanine ammonia-lyase (PAL) and SOD activity responded positively to the Si treatment. Silicon application raised phenylalanine ammonia-lyase activity and total phenol concentrations led to increase the fruit’s ability to better withstand stressful cold impacts. In addition, Si considerably reduced the rise in malondialdehyde (MDA) content during storage, obviously because of an efficient scavenging following significant enhancement of SOD activity. Conclusion: Postharvest Si application raised antioxodant activity and total phenolic, reduced malondialdehyde content and lipid peroxidation, and led to increase the fruit’s ability to better withstand stressful cold impacts. These results suggest that Si application may be recommended for increasing storage life and maintaining their quality.}, Keywords = {phenylalanine ammonia-lyase, plum, silicate, superoxide dismutase, total phenol}, volume = {5}, Number = {19}, pages = {95-100}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-656-en.html}, eprint = {http://ncmbjpiau.ir/article-1-656-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Kasiri, Masoumeh}, title = {Study of the efficacy pegylated Cisplatin loaded nano archaeosome on breast cancer in vitro}, abstract ={Aim and background: Archaeosome nanoparticles are used in drug delivery to improve the efficacy of therapeutic agents and decrease the side effects. In this study, archaeosome nanoparticles were used as Cisplatin carrier. Material and methods: Methanogenic archaea were successfully cultured in a routine liquid medium. Archaeosome was extracted from the membrane of archaea using centrifugation process. After that homogenous emulsion of archaeosome was raised in phosphate buffer saline containing poly ethylene glycol and Cisplatin under motion by stirrer. Sonication performance was also recruited to obtain the nano sized archaeosome and Cisplatin entrapment. While Cisplatin encapsulation efficiency was estimated in supernatant after separation by ultracentrifuge, cytotoxicity effects of Cisplatin in the free form and encapsulated on the archaeosome were calculated in the breast cancer MCF-7 cell line using MTT assay. Results: Drug-containing nanoparticles were synthesized successfully. The entrapment efficiency for pegylated and non pegylated nano archeasomes was estimated 70.16 and 54.52% respectively. Size of the nanoparticles was shown to be 132/84 and 388.31 nm for non pegylated and pegylated nanoparticles respectively. Although cytotoxicity effects of Cisplatin was increased when drug become associated with NPs, this effect was predominant when pegylation process was contributed to construct the Cisplatin loaded nano archaeosome. Conclusion: The results of our study suggested that archaeosome nanocarriers could be used as a suitable carrier for Cisplatin while pegylation increases the efficiency of preparation.}, Keywords = {Drug delivery, Archaeosome, Cisplatin, Breast Cancer }, volume = {5}, Number = {19}, pages = {101-108}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-659-en.html}, eprint = {http://ncmbjpiau.ir/article-1-659-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {hejazi, Zahra and Abbaszadegan, Mohammad Reza and Gholamin, Mehran and Forghanifard, Mohammad Mahdi}, title = {Isolation and Cloning of MAGEA4 human gene into pRUF expression plasmid}, abstract ={Aim and background: Cancer/testis (CT) antigens are a category of tumor antigens that are typically expressed only in the human germ line, and in several types of tumor. MAGEA4 is one member cancer testis antigen family that has relation with other tumors. Since the biological function of MAGEA4 is unclear, the aim of the present study was amplification and cloning of the gene in the expression vector to produce recombinant protein that expresses MAGEA4. Material and method: Using PCR specific primers including restriction site, MAGEA4 was amplified. The purified PCR products were ligated between the BamH1 and Xho1 sites of pTZ57/R cloning vector and transformed into Escherichia coli Top10 strain and screened by IPTG and X-Gal. The correct orientation of MAGEA4 fragment was identified by restriction enzyme analysis and sequencing of constructed plasmid. Then sub cloning was carried out within pRUF expression vector with the same restriction site. Result: The final confirmation was performed using colony PCR, double digesting and sequence analysis. Therefore the MAGEA4 gene was cloned into the restriction site of pRUF. Conclusion: This study is an important step for producing recombinant proteins and is used to find the function of this gene for therapeutic targets.}, Keywords = {Cancer Testis Antigen, Cloning, MAGEA4, Expression vector pRUF}, volume = {5}, Number = {19}, pages = {109-114}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-660-en.html}, eprint = {http://ncmbjpiau.ir/article-1-660-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Saberi, Fatemeh and Babaei, Delaram and Malmir, Somayeh and MoosazadehMoghaddam, Mehrdad and Mirnejad, Rez}, title = {Survey of the commercial value and sale of biotech pharmaceutical products}, abstract ={In the past two decades, given the importance of biotech drugs and its effective role in the treatment of many diseases, the commercialization and selling of these products is dramatically grown worldwide and many large companies have obtained macro-economic benefits for their own and their mother country by investing in this area. The United States is the largest investor in the research, production and sales of pharmaceutical products derived from biotechnology therefore, studying the biotechnological drugs market can provide an overall vision about the commercialization process and the market for these types of drugs in the world. For the young researchers in biotechnology and pharmaceutical field to be familiar with commercial status and sales of biotechnological drugs, the business development and selling of these products in the United States in a period of 10 years (2002-2012) was studied as a reference model. Data presented in this article is based on the reports from 2007 to 2014, which are published in each year in the Nature Biotechnology Journal.}, Keywords = {Biotechnology, Drug, Business, Sale}, volume = {5}, Number = {20}, pages = {9-22}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-694-en.html}, eprint = {http://ncmbjpiau.ir/article-1-694-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Naseri, Fatemeh and Daemii, Mohammad Reza and Sangtarash, Mohammad Hossein and Torkamanzehi, Adam}, title = {Detection and Comparison of Sequences of VP28 Gene of White Spot Syndrome Virus Affecting Litopenaeus vannamei in Chabahar (Iran)}, abstract ={Aim and Background: White spot syndrome virus (WSSV) infection has resulted in severe production and economic losses in shrimp culture globally. The first time the white spot disease (WSD) was reported in Iran was in 2004 and 2008, respectively in Bushehr Province. In Sistan and Balochestan province only the WSD was reported in 2008. White spot syndrome virus (WSSV) is the most pathogenic among the penaeid shrimp viruses. Materials and Methods: WSSV DNA samples for PCR test were prepared from diseased shrimp. Specific PCR primers were used for the amplification of VP28 gene in WSSV. VP28 gene of DNA isolate of WSSV was amplified by PCR and the PCR product was sequenced. Results: The results were submitted to GenBank (KF723558). The nucleotide sequence (KF723558) was compared with the VP28 gene sequences in the GenBank database (NCBI). Conclusion: 36 sequences showed significant alignments with 99% homology which include the sequences from Korea, Japan, US, Indonesia, China, Vietnam, Taiwan, the Netherlands and Thailand. However, the homology was not observed 100% in the sequences.}, Keywords = {White Spot Syndrome Virus, Polymerase Chain Reaction, shrimp, GenBank database}, volume = {5}, Number = {20}, pages = {23-28}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-695-en.html}, eprint = {http://ncmbjpiau.ir/article-1-695-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Hosseinian, Zhila and Rasouli, Rahimeh and Azarnoosh, Amin and Mortazavi, Mehri and Akbarzadeh, Azim}, title = {Evaluation of magnetic nanoparticles loaded with cisplatin Performance on breast cancer in In vivo and in vitro studies}, abstract ={Aim and Background: Today, the use of magnetic nanoparticles of iron oxide is highly regarded in various fields of medicine such as imaging and drug delivery. In this study magnetic nanoparticles with cisplatin as chemotherapy drug have been studied as an efficient drug delivery system with lower doses of the chemotherapeutic agent, the control of drug distribution in the body and destruction of breast cancer cells. Materials and Methods : In the present study, iron oxide magnetic nanoparticles containing cisplatin were synthesized. The size and morphology of nanoparticles were characterized. Finally, in-vivo and in-vitro cytotoxic effects on MCF-7 cell lines and animal models Balb/c mice were evaluated. In vitro effects of cisplatin and magnetic nanoparticles-loaded with cisplatin on MCF-7 cell line were evaluated. In vivo study of different doses of nanoparticles loaded with cisplatin and cisplatin on Balb/c mice with breast cancer were studied. Changes in tumor size were evaluated by calculation of tumor volume. Results : The results showed that nanoparticles loaded with cisplatin in human breast tumor tissue affect effectively. Magnetic nanoparticles loaded with cisplatin compared to free cisplatin showed higher toxicity effects with lower doses of cisplatin on MCF-7cell line. Conclusion : The results showed that the magnetic nanoparticles loaded with cisplatin can be proposed as an appropriate candidate for the treatment of breast cancer with minimal side effects.}, Keywords = {nanoparticles, PEGylation, cisplatin, MCF-7, breast adenocarcinoma}, volume = {5}, Number = {20}, pages = {29-36}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-696-en.html}, eprint = {http://ncmbjpiau.ir/article-1-696-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Shahabi, Javad and EbrahimiShahmabadi, Hasan and Alavi, Seyed Ebrahim and KoohiMoftakhariEsfahani, Maedeh and Ardjmand, Mehdi and Seyfkordi, Aliakbar and Akbarzadeh, Azim}, title = {Effect of gold nanoparticle on properties of liposomal hydroxyurea: in vitro study}, abstract ={Aim and Background: Functional nanoparticles have offered many hopes in drug delivery. These structures not only increase the efficiency of nanoparticle-associated drugs, but also attenuate the side effects of these therapeutic agents. In this study various nanoparticulate formulations of Hydroxyurea (HU) were constructed. Materials and Methods: reverse phase evaporation technique was used to obtain the Hydroxyurea-loaded liposome. Aspartic acid containing-Gold nanoparticles (GNPs) were manufactured using reduction of chloroauric acid salt. DNA extracted from human breast cancer cell line MCF-7 was then conjugated to GNPs (nanoconjugate). Nanoconjugate was subsequently mixed with Hydroxyurea-loaded liposome to give nanoconjugate complex. Obtained nanoparticulate formulations were characterized with dynamic light scattering (DLS) and UV-Vis spectrophotometery methods. Spectrophotometery was also used to determine the drug loading efficiency. MTT assay and MCF-7 cell line was contributed to provide the information about cytotoxicity effects of various formulations. Results: Drug loading efficiency was found to be 70%. While nanoconjugate complex gave the largest size with 502 nm, size of produced GNPs was minimum with 29 nm. Although compared to free drug, efficiency was significantly enhanced when HU was coupled to nanoparticulate formulations, this effect was more evident at lower concentrations of drug (>20 µM). In this regard cytotoxicity effect of nanocomplex was prominent. Cytotoxicity effects of HU-loaded liposome and nanoconjugate complex at lowest concentration (5 µM) were estimated to be 70 and 81 percent respectively. More cytotoxicity observed with nanoconjugate complex could be attributed to an increase in the HU transfer to cell because of GNPs. Free HU showed cytotoxicity effects equal to 32 and 88 percent at 5 and 2500 µM, respectively. Conclusion: Results of the study showing that liposome nanoparticle could be considered as a suitable carrier for HU. It has demonstrated that GNPs exert essential role in which at the present of this nanoparticle as nanocomplex structure, cytotoxicity effects were significantly increased. According to these observations, use of GNPs as liposomal nanocomplex for various drug formulations could be considered. Because of significance increase in the cytotoxicity effects of nanoconjugate complex produced in this study, it is recommended to initiate the in vivo studies.}, Keywords = {Gold nanoparticle, liposome, Hydroxyurea, drug delivery}, volume = {5}, Number = {20}, pages = {37-46}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-697-en.html}, eprint = {http://ncmbjpiau.ir/article-1-697-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Asgari, Fatemeh and Falak, Reza and Bahar, Mohammad ali and majd, Zahra and Heydarinasrabadi, Mitra and Dashti, Somayeh and Shekarabi, Mehdi}, title = {The evaluation of oral probiotic feeding on T cell subsets infiltration in chicken as experimental model.}, abstract ={Aims and background: This study was performed to evaluate the effect of lactobacillus acidophilus Bacteria (LAB) on chicken T cell subsets at ileum, bursa and cecal tonsil tissues. Material and Methods: Thirty chickens were divided into three groups and fed with sterilized cow milk (M), or a mixture of M and LAB, kept as controls. Chickens from each group were sacrificed after 2 or 3 weeks and lymphoid tissues including ileum, bursa and cecal tonsil were removed and cryo preserved. The ratio of T cell subsets including CD4+, CD8+ and gamma delta T cells were studied by immune-histochemistry method. Results: The ratio of CD4+ T cells was significantly higher in ileum of chicken after 2 weeks of probiotic intake. Moreover, this ratio was even higher than the ratio of these cells in the third week. In the cecal tonsil sections the percentage of gamma delta T cells was higher in milk fed chickens, after 3 weeks. conclusion: The findings indicate that feeding of chickens with probiotics may alter the distribution of T cells in the lymphoid tissues. However, coincidental increase of CD4+ and gamma delta T cells after 2 and 3 weeks of treatment indicates that these effects are transient and could not have a permanent effect on avian mucosal immunity.}, Keywords = {probiotic, chicken, T lymphocyte, lactobacillus acidophilus}, volume = {5}, Number = {20}, pages = {47-54}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-698-en.html}, eprint = {http://ncmbjpiau.ir/article-1-698-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Eizadkhah, Parisa and hosseini, Seyed Davood and Purbabai, Ahmad Ali}, title = {Molecular identification of Ornithobacterium isolates from poultry in Markazi Province}, abstract ={Aim and Background: Ornithobacteriosis is an infectious disease of avian species reported in almost all countries around the world. The first recorded isolation of ORT was made from turkeys in Germany in 1981. ORT has been isolated from chickens, ducks, geese, guinea fowls, gulls, ostriches, partridges, pheasants, pigeons, quails, rooks and turkeys so far. In Iran, ORT infection was reported by Banani et al for the first time. The aim of the study was to identify the Ornithobacterium isolated in Markazi province using molecular analyses. Materials and Methods: Sampling and biochemical isolation has been performed in Razi vaccine and serum research institute. Random sample of 20 units and 231 birds has been collected from the Arak poultry farms during August 2011 to March 2013. All of the fifteen isolated samples were cultured in blood agar media with 5µg/ml Gentamicin and incubated at 48 hours in 37˚C. Then all samples were subjected to PCR and sequencing for 16S rRNA. Results: PCR results and observations 784 bp band on the agarose gel confirmed the existence of genera Ornithobacterium. Also compared sequences obtained from isolated sequences available in GeneBank showed that 98-100% were similar to Ornithobacterium rhinotracheale strain indicating the existence of these bacterial species in the farms of Markazi province. Conclusion: Ornithobacterium rhinotracheale can be a dominant species from Ornithobacterium in the farms of Markazi province.}, Keywords = {ORT, PCR, poultry, 16S rRNA, respiratory disease}, volume = {5}, Number = {20}, pages = {55-62}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-699-en.html}, eprint = {http://ncmbjpiau.ir/article-1-699-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Darvishzadeh, Fariborz and nejatzadeh, Fatemeh and Iranbakhsh, Ali rez}, title = {Effects of silver nanoparticles on salinity tolerance in basil plant (Ocimum basilicum L.) during germination in vitro}, abstract ={Aim;Background: Basil (Ocimum basilicum) belongs to the family Lamiaceae. It is annual plant known as an important medicinal plant. To study the effects of nano-silver particles on salinity tolerance behaviors (Ocimum basilicum L.), the present research was conducted. Materials and Methods: experiments were carried out factorially based on CRD with four replicates during autumn 2013. Samples were treated with 6 levels of nano-silver particles, including 0, 20, 40, 60, 80 and 100 mg.kg-1 and 6 levels of salinity 0, 30, 60, 90, 120, 150, 180 mmol.l-1. Salinity treatments were prepared using NaCl and CaCl2 at the rate of 1:1 in Hogland solution of 50%. The germination percentages, germination speed, vigor index, were also determined. Results: Results showed that the application of nano-silver particles at the concentration of 40 mg.kg-1 led to the increases in germination percentage and improved the resistance to salinity conditions. Conclusion: the application of nano silver at suitable levels during germination process may enhance germination traits, plant growth and resistance to salinity conditions in basil seedlings.}, Keywords = {(Ocimum basilicum L.), Germination, Nano silver particles, Salinity}, volume = {5}, Number = {20}, pages = {63-70}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-700-en.html}, eprint = {http://ncmbjpiau.ir/article-1-700-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {FatehiGharehlar, Laleh and Yaghmaei, Parichehreh and Parivar, Kazem and Ebrahim-Habibi, Azadeh and Maleki, Masou}, title = {The study of the role of GABAa receptors during fetal development on the levels of cytokines in the hippocampus and prefrontal cortex of NMRI mouse offsprings.}, abstract ={Aim and Background: Many studies have found an association between inflammation and mental disorders such as anxiety and depression in different regions of the brain. Inflammatory factors cause increase in cytokines levels. On the other hand elevated levels of cytokine are associated with several neurological disorders, including anxiety, depression, Alzheimer and Parkinson's diseases. GABA (Gamma Aminobutyric acid) is a major inhibitory neurotransmitter in the mammalian central nervous system. Many studies have proven the role of GABA in mental disorders including anxiety, depression. The present study investigates the effects of GABA agonists and antagonists on the levels of inflammatory factors and cytokines levels in offsprings. Materials and Methods: In the present study, the pregnant female rats in their late pregnancy were treated for 3 days, from 16th to 18th day of gestation, with the antagonist or agonist GABAa receptor with 10, 50, 100, 500 and 700 μg / kg of Muscimol and 20, 70, 140, 500 and 800 μg / kg of Bicuculline. After the treatment, the cytokines levels in the hippocampus and prefrontal cortex were measured in offsprings. The measurement was carried out by ELIZA technique. Results :Our results showed that the treatment on pregnant female rats in their late pregnancy (16th to 18th day of gestation for 3 days( with antagonist (bicuculline) or agonist (muscimol) of GABAa receptor which were 500 and 700 μg / kg for muscimol 140 and 500 μg / kg for bicuculline, had no effect on the levels of cytokines. Conclusion: In conclusion, these studies showed that the blockage of GABA receptor plays no role in the inflammatory factors and cytokines.}, Keywords = {GABAA-‌R, Cytokine, rat}, volume = {5}, Number = {20}, pages = {71-78}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-701-en.html}, eprint = {http://ncmbjpiau.ir/article-1-701-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Sepehri, Nahid and Mahmoodi, Mohammad Mehdi}, title = {Production of pyocin by Pseudomonas aeruginosa and investigation of its synergism effect with some common antibiotics in vitro}, abstract ={Aim and background: Pseudomonas aeruginosa is an important cause of nosocomial infection. According to widespread antibiotic resistance and limitation of effective drugs, nowadays much attention has been focused on the use of alternative drugs. One of the alternatives is pyocin of Pseudomonas that can have inhibitory effect on relative species and even on other genus. This study was aimed to study the production and investigation of antimicrobial effects of pyocin on some pathogenic bacteria and investigation of its synergism effect with some common antibiotics. Materials and methods: Production of pyocin by isolated strains of Pseudomonas aeruginosa was investigated without stimulator and in presence of mitomycin C in various temperature and pH values. Inhibitory effect of pyocin was studied on some pathogenic bacteria also synergism effect of pyocin with some antibiotics was investigated. Results: Pyocin production was done both in the absence of stimulator and to a greater extent in the presence of mitomycin C. Optimum temperature for the production of pyocin was 37C and optimum pH was 7. Produced pyocin showed a significant inhibitory effect on target bacteria and had synergism effect with some studied antibiotics. Conclusion: Pyocin can have an efficient inhibitory effect on pathogenic bacteria in vitro. Upon further investigations especially in vivo, more detailed information can be obtained about the possibility of its using as an alternative or complement for the treatment of bacterial infections.}, Keywords = {Pyocin, mitomycin C, synergism, Pseudomonas aeruginosa}, volume = {5}, Number = {20}, pages = {79-88}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-702-en.html}, eprint = {http://ncmbjpiau.ir/article-1-702-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {BazelliMahbob, Fatemeh and Attar, Hosien and Amoabediny, Ghassem and Iman, Maryam}, title = {Encapsulated of ascorbic acid in the nanomicelle}, abstract ={Aim and Background: Ascorbic acid (vitamin C) is a white powder and non-toxic. VC is the most important antioxidant. This vitamin is not stable enough in aqueous solution. The aim of this work is to encapsulate ascorbic acid within nanomicelle in order to increase the stability of vitamin, fat-soluble and higher concentrations drug delivery. Materials and Methods: Using surfactant Tween 80 and α-tocopherol in stable temperature conditions, nanomicelle was developed. Encapsulation efficiency was calculated by spectrophotometric method. The morphology of nanomicelle was examined with electron microscope TEM operating and Size measurements of the samples were obtained by Dynamic Light Scattering Instrument (Nano-ZS Malvern, UK). Results: The mean diameter and encapsulation efficiency of nanomicelle ascorbic acid is, respectively, 100 nm and 70%. Conclusion: This study showed that ascorbic acid can be encapsulated into nanomicelle and protected against environmental factors such as temperature, humidity, pH and light also with this method the fat-solubility of vitamin can be achieved.}, Keywords = {ascorbic acid, encapsulated, nanomicelle, vitamin C, Tween80}, volume = {5}, Number = {20}, pages = {89-96}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-703-en.html}, eprint = {http://ncmbjpiau.ir/article-1-703-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Rasaneh, Samira and Rajabi, Hosein and Heydari, Samir}, title = {The biological properties evaluation of the radiolabeled complex: 99mTc -DTPA- Herceptin in cancerous cell lines}, abstract ={Aim and Background: Herceptin as a humanized monoclonal antibody against HER2 is used for the treatment of the early stage of HER2 over-expresed breast cancers. This antibody has the potential to be used as radioimmuno-scintigraphy agent in diagnosing HER2 breast cancer. In this study, the radiolabeling of Herceptin with 99mTc was evaluated for the first step in producing a new radiopharmaceutical agent. Materials and Methods: Herceptin antibody was labeled with Technetium-99m using Diethylene Triamine Pentaacetic Acid (DTPA) as chelator. All the quality control tests were performed. Labeling efficiency, stability in buffer and serum, immunoreactivity, toxicity and cell binding of the complex were tested on different breast cancer cell lines. Results: The labeling efficiency of the complex was 97 ± 2%. The stabilities in phosphate buffer and in human blood serum within 24 h post preparation were 96 ± 1% and 90 ± 2%, respectively. The immunoreactivity of the complex was 83 ± 3%. The binding of labeled antibody to the surface of SKBR3, MCF7 and A431 cells were increased by increasing Her2 concentration on the cells surface. No cytotoxicity effect of complex was seen on the cell lines. Conclusion: The findings showed that 99mTc-DTPA-Herceptin can be a promising candidate as HER2 antigen scanning for human breast cancer that needs more investigations in future.}, Keywords = {Herceptin, HER2, breast cancer, 99mTc}, volume = {5}, Number = {20}, pages = {97-104}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-704-en.html}, eprint = {http://ncmbjpiau.ir/article-1-704-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {asadi, elham}, title = {The effect of glucose and citric acid on Bovine sperm motility patterns}, abstract ={Aim and background: Sperm motility is a critical factor for fertilization. Glucose and citric acid can be metabolized by these cells. We study the role of these two on Bovine sperm motility in BO medium. Materials and method: Testicles were removed from tunica albuginea. Epididymes were incised and 4-6 *106 sperm cells/ml were transferred into BO medium containing different concentrations of glucose (G1 & G2) with or without citric acid, and incubated in a %5 Co2 incubator in humid air at 38 C for up to 8 hours. Sperm cells were observed under CASA system (computer assisted sperm analyzer) and data for the Class A, Class A+B, Live ratio, Straight Line Velocity (VSL), Curvilinear Velocity (VCL), Average Path Velocity (VAP), Beat Cross Frequency (BCF) were recorded. Result: The results showed that Class A was significantly higher at G1 & citric acid at 6 hours post incubation (p<0.05). Class A+B was significantly higher at G1 & citric acid at 4 & 6 hours post incubation (p<0.05). Live-ratio was significantly higher at G1 & citric acid too. Such effects didn’t observe at G2 & citric acid medium. Conclusion: The results showed that citric acid has an increasing effect on these parameters. This effect can be affected by concentrations of glucose. Our results didn’t show any significant difference in comparing motility patterns at G1 and G2 concentrations of glucose with or without citric acid. This part of the results suggests that citric acid has no any effect on these parameters.}, Keywords = {Epididymal sperm, Glucose, Citric Acid, viability, velocity}, volume = {5}, Number = {20}, pages = {105-112}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-705-en.html}, eprint = {http://ncmbjpiau.ir/article-1-705-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {HashemiShahraki, Abdolrazagh and ZakerBostanabad, Saee}, title = {Identification of potentially pathogenic Nontuberculous mycobacteria isolated from drinking water systems in Tehran}, abstract ={Aim and Background: Nontuberculous mycobacteria (NTM) are widely dispersed in the environment and are commonly isolated from environmental sources. Several species of environmental mycobacteria have been known to be important as human pathogens. The aim of this study was the isolation and molecular identification of NTM recovered from Tehran drinking water samples. Material and Methods: From 22 different municipality regions of Tehran, 44 water samples were collected and passed through 0.45 μm filters. Filters were rinsed, treated with NaOH and SDS and transferred onto Lowenstein-Jensen medium. The isolates were subjected for molecular identification by sequencing rpoB gene. Results: All of the collected waster samples were positive for NTM. Three samples had two types of NTM while the rest had one NTM isolate in pure culture. Contamination rate was 1%. Total of 47 isolates were isolated. Using rpoB gene sequencing M. simiea, M. fortuitum and M. gordonae were most frequent encountered species. Conclusion: Our findings showed NTM are the most common inhabitant bacteria in drinking water systems. This study provided impotent evidence about distribution of NTM in environmental sources.}, Keywords = {NTM, identification, drinking water, Tehran}, volume = {5}, Number = {20}, pages = {113-118}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-706-en.html}, eprint = {http://ncmbjpiau.ir/article-1-706-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} } @article{ author = {Salehian, Pirooz and Hassani, Masoumeh and Naderi, Maryam and Pooladi, Arash}, title = {Comparison of XRCC1Arg399Gln Polymorphism in Endometriosis and Healthy Women}, abstract ={Aim and Background: XRCC1encods the protein involved in base excision repair (BER) system. Because of important role of XRCC1 gene, polymorphism in this gene could increase the risk of many neoplasms in human. We conducted a research project to find out the association between endometriosis with XRCC1polymorphism. Materials and Methods: In this cross sectional study 102 cases were enrolled. DNA was isolated from peripheral blood, and then genotyping of codon 399 of XRCC1 was carried using specific primers and RFLP method. Results: Data from102 cases in an endometriosis group (n=52) and a control group (n=50) were analyzed and led to the following. results: the Arg399Gln polymorphism of XRCC1 gene was 76.9% in the endometriosis group compared with 28% in the control group (P=0.0001) with OR= 2.47 and 95% CI: 1.72–4.39 (for GG vs. GA+AA) using Fisher’s exact test. Allelic Frequency on the Endometriosis group was 57.69% for G and 42.31% for A allele these rates for the control group were 86% and 14% for G and A alleles respectively (P=0.0001). Conclusion: we came to the conclusion that arginine to glutamine polymorphism of XRCC1 gene codon 399 is associated with an increase in the risk of endometriosis and obtained odd’s ratio indicates that people with this polymorphism have 2.47 times the risk of developing endometriosis compared to people without polymorphism}, Keywords = {Arg399Gln polymorphism, Endometriosis, PCR-RFLP, XRCC1}, volume = {5}, Number = {20}, pages = {119-125}, publisher = {Islamic Azad University of Parand}, url = {http://ncmbjpiau.ir/article-1-707-en.html}, eprint = {http://ncmbjpiau.ir/article-1-707-en.pdf}, journal = {New Cellular and Molecular Biotechnology Journal}, issn = {2228-5458}, eissn = {2228-6926}, year = {2015} }