2024-03-28T15:34:43+03:30 http://ncmbjpiau.ir/browse.php?mag_id=3&slc_lang=fa&sid=1
3-3 2024-03-28 10.1002
New Cellular and Molecular Biotechnology Journal NCMBJ 2228-5458 2228-6926 - 2011 1 1 Investigation of Genetic Diversity in Cotton Cultivars using RAPD Markers Farzaneh Tafvisi Massoud Sheidaei Farah Farahani Aim and Background. Cotton is considered as one of the most important crop plants in Iran, cultivated in various regions of the country. Continuous cultivation of the same genotypes may bring about genetic erosion in the long term therefore study of the available genetic variability as well as introducing the new ones is of importance.Materials and Methods. Molecular study was performed in 10 tetraploid cotton cultivars (Gossypium hirsutum L.S). Random Amplified Polymorphic DNA (RAPD) profiles for ten cotton genotypes were generated with 30 random primers. Results. Out of 30 primers used 22 primers produced reproducible bands. The primers generated 387 RAPD loci, of which 178 were polymorphic. The primer OPM11 produced the highest number of Polymorphic bands and Primer OPC90 produced the highest number of unique bands while some of the primers produced no unique band at all. Siokra cultivar produced the highest unique bands (6).Conclusions. NJ clustering methods performed on molecular data. Two parental cultivars of Nazilli and Siokra are grouped together with 100% bootstrap value in NJ dendrogram.  Gossypium hirsutum L. genetic diversity RAPD markers 2011 1 01 7 14 http://ncmbjpiau.ir/article-1-3-en.PDF
3-4 2024-03-28 10.1002
New Cellular and Molecular Biotechnology Journal NCMBJ 2228-5458 2228-6926 - 2011 1 1 Study of Mycobacteria lInfection in Hematological Malignancies using Culture and PCR Method of Bone Marrow Aspiration Saba Nasr Mohammad Karim Rahimi Saeed Zaker Bostanabad Seyed Asadollah Mousavi Parvaneh Adimi Shahin Pourazar Dizaji Aim and Background. Patients with hematological malignancies are at higher risk for mycobacterial and many other opportunistic infections due to immunosuppression that resulted from dysfunctional blood cells and chemotherapy-induced depletion in cells of immune system. This study was performed to evaluate the frequency and contributing factors for mycobacterial infections in patients with hematological malignancies according to the both culture and PCR methods on bone marrow aspiration.Materials and Methods. In this cross-sectional descriptive study, 96 patients with hematological malignancies attending to tertiary health care centers in 2009 and 2010 were enrolled. The bone marrow aspiration was cultured and also evaluated by PCR method.Results. The mean age of the patients was 39.49 years and 64.6 percent were male. Acute myeloblastic leukemia (23.3%), acute lymphoblastic leukemia (19.2%), and multiple myeloma (17.8%) were the most common malignancies. The cultures of bone marrow aspiration were negative for mycobacterium species in all patients but nucleic acid of this bacterium was detected by PCR method in three cases (3.1%) including two patients with acute lymphoblastic leukemia and one patient with multiple myeloma. Aspergillus, streptomyces, and brucella species.were found in culture of two, one, and two patients, respectively. The age, sex, type of malignancy, and bone marrow transplantation had no effect on PCR results (P > 0.05).Conclusions. The frequency of mycobacterial infections in patients with acute lymphoblastic leukemia and multiple myeloma may be higher than other hematological malignancies. However for decreasing the morbidity and mortality in these patients monitoring of bone marrow aspiration for mycobacterial and other opportunistic infections is suggested. mycobacterial infection hematological malignancy bone marrow aspiration PCR culture 2011 1 01 15 19 http://ncmbjpiau.ir/article-1-4-en.PDF
3-5 2024-03-28 10.1002
New Cellular and Molecular Biotechnology Journal NCMBJ 2228-5458 2228-6926 - 2011 1 1 Study the Probiotic Effects of Lactobacillus Plantarum on Cutaneous Wound Healing in Rat Mitra Heydari nasrabadi Maryam Tajabadi ebrahimi Shokofeh Dehghan Bonadaki Mansoreh Torabi kajousangi Farimah Zahedi  Aim and Background. Probiotics are microorganisms in the gastrointestinal tract that besides helping the digestion of complex molecules, also helped the immune system and will reduce inflammation. The beneficial effects of these bacteria on ulcer healing has been proved but little research has been don done in the field of healing effects of these bacteria on cotaneous wound healing. Therefore, the purpose of this experiment is study the effect of lactobacillus plantarum isolated from Iranian traditional dairy products on cutaneous wound healing. Materials and Methods. After isolated 22 strains of lactobacillus from Iranian traditional-dairy products and measuring exopolysaccharide production in stains by phenol-sulfuric acid method eventually, Lactobacillus plantarum selected because it has a high exopolysaccharide (EPS) production. Wistar male rats divided into 3 groups experimental, control and negative control (n=5) and a full-thickness wound   (1.5 × 1.5 cm) was made on the back of each rat (45 rats in 3 groups). After 24 hours of injury, Two groups, control and experimental, were treated by eucerin and eucerin contained lactobacillus plantarum, respectively, but the negative-control group did not receive anything. On days 1, 3 and 14 rats were killed and wound samples were collected for histological and statistical studies. Results . Lactobacillus plantarum significantly decreased wound area compared to control and negative control groups and increased wound healing. Histological study on day 3 showed significant increase in  number of neutrophils and significant increases in macrophages and fibroblasts (p<0.001). Also significant reduction in neutrophils, macrophages and fibroblasts numbers observed on day 14 (p<0.001). Conclusions : The current study presented a significant decrease in inflammation and an acceleration of wound healing in Lactobacillus plantarum treated rats as compared to the control and negative control groups. Further studies are required to develop a detailed mechanism of Lactobacillus plantarum during cutaneous wound healing.   Cutaneous wound Probiotic Healing Lactobacillus palantarum Exopolysaccharid 2011 1 01 21 27 http://ncmbjpiau.ir/article-1-5-en.PDF
3-6 2024-03-28 10.1002
New Cellular and Molecular Biotechnology Journal NCMBJ 2228-5458 2228-6926 - 2011 1 1 Comparative study of Intrauterine Listeriosis by Indirect Immunofluorescence of Serum Antibody and Amniotic Fluid Culturing in Aborted Mother and Preterm Labour Mohammad Karim Rahimi Zhila Amirkhani Parvaneh Adimi Lida Mousavi Zahra Tayebei Mozhgan Masoumi Tahereh Fazlalikazemi Zeinab Mahmoodi Parivash Ebrahimi Nepton Nickanjam Aim and Background . Listeria monocytogenes is the ethiologic agent of abortion, preterm labour, neonatal septicemia and meningitis. The aim of this study was to determine the prevalence of listeriosis in aborted mothers or preterm labour. Two methods of indirect immunofluorescens (IF) and amniotic culturing also were compared.    Materials and Methods . This is an analytical study for evaluation of laboratory tests. In this research, the blood samples were collected from mothers and their serum were tested by indirect IF for anti-listerial antibodies. Products of aborted fetus or preterm labour (amniotic fluid or a piece of placenta) were obtained as a specimen for culturing.  Results . In this study, 518 mothers, 29 cases (5.59%) admitted with preterm labour, 125 cases (24.1%) with repeated abortion and 364 cases (70.31%) with first time abortion. Six cases (1.15%) had higher titer of   anti-listeria antibody (1/800) in indirect IF but in 5 cases (0.96%) L.monocytogenes was detected in culture. Conclusions . Indirect IF should be confirmed by culturing of infected specimens. Determining of   anti-listerial antibody in pregnant women and selection of listeriosis for antimicrobial treatment may prevent the spontaneous abortion, still birth and preterm labour. Therefore, we suggest monitoring L.monocytogenes seropositivity in pregnant women with high risk of threatened abortion, and also microbiological assessment of symptomatic women for detection of L.monocytogenes and insidious infection.      Indirect Immunofluorescence Amniotic fluid Culturing Abortion Preterm labour 2011 1 01 29 37 http://ncmbjpiau.ir/article-1-6-en.PDF
3-8 2024-03-28 10.1002
New Cellular and Molecular Biotechnology Journal NCMBJ 2228-5458 2228-6926 - 2011 1 1 Study of morphogenesis of Heliotropium chorassanicum Bung (Heliotropiaceae) Gholamreza Bakhshi Khaniki Narges Moradtalab Behrouz Shahsavan Behboodi  Aim and Background . Heliotropium chorassanicum is an endemic species to Iran which has not been carefully investigated . It is widespread in Kopetdagh area in Khorasan province . In this study anatomical & cytological structure of root , shoot , leaf , flower and its meristem and fruit of above mentioned species has been investigated . Materials and Methods . The samples was collected from Kharasan - e Razavi province and after fixation by FAA fixator , stained by methylgreen & karmen hematoxyline . To understand its structure , pictures have been taken by light microscope . Results . The results show that leaf blade has epiderm with frequent trichomes cuticule very narrow and thin mesophyll with chlorophyll , isobillateral in both surfaces , bundle sheath is lanceolate and phloem with 2-6 cell layer stomata mostly concentrated at hypoderm . Conclusions . With pay attention to identification key and comparition of leaves and trichomes in this species it is conclude that the trichomes are simple with cupshape end and clear cell base . The root has secondary growth .    Heliotropium chorassanicum morphology morphogenesis 2011 1 01 39 44 http://ncmbjpiau.ir/article-1-8-en.PDF
3-9 2024-03-28 10.1002
New Cellular and Molecular Biotechnology Journal NCMBJ 2228-5458 2228-6926 - 2011 1 1 Biochemical Markers Method for Diagnostic of Tuberculosis in the Pleural Samples from Iranian Patient Saeed Zaker Bostanabad Donya Khosravani Mostafa Ghalami Mohammad Karim Rahimi Iman Fallah Hani Ansari  Aim and Background. To compare the diagnostic efficiency of adenosine deaminase, isoenzyme adenosine deaminase-2 and concentration of interferon-γ in patients with tuberculous pleural effusion.   Materials and Methods. The prospective study was done on 114 patients who were divided into 3 groups: tuberculous, non-tuberculous infectious pleurisy, and malignant effusion. The adenosine deaminase, adenosine deaminase-2 and interferon-γ were analyzed by receiver operating characteristic curves. Results. There was increase of all three markers in tuberculous pleural effusion but not in nontuberculous effusion. The cut-off values for adenosine deaminase, adenosine deaminase-2 and interferon-γ were 40, 26 U/l. and 299 pg/ml respectively. Adenosine deaminase, adenosine deaminase-2 activities were  significantly higher in tuberculous effusion than in malignant pleural effusion (more than 5 times) and in non-tuberculous infectious pleurisy (more than 4 times). The median of interferon-γ concentration in pleural fluid of tuberculous patients was 1514.2 pg/ml (931.2-2187.5 pg/ml) which was 10 times more than the median values of other groups of patients. There was no significant difference between patients with malignant effusion and those with non-tuberculous pleural effusion. Conclusions. ADA and ADA2 in pleural is a marker for diagnostic of pleural effusion and all three markers had higher diagnostic yield for tuberculous effusion with 95% sensitivity.    Adenosine deaminase isoenzyme interferon γ tuberculous pleural effusion 2011 1 01 45 52 http://ncmbjpiau.ir/article-1-9-en.PDF
3-10 2024-03-28 10.1002
New Cellular and Molecular Biotechnology Journal NCMBJ 2228-5458 2228-6926 - 2011 1 1 Effects of histamine on anxiety-related behavior in morphine sensitized and naïve rats Parvin Khodarahmi Shahrbano Oryan Aim and Background. It has been suggested that histamine have modulator influence on anxiety-related behaviors both in animals and humans. On the other hand, ventral hippocampus (VHC) may be an important brain site in the modulation of fear or anxiety.  Materials and Methods. In the present study, the effects of bilateral intra-VHC injections of histamine on anxiety-related behavior have been investigated in morphine-sensitized and naïve rats using a plus-maze model. Sensitization was obtained by subcutaneous injections of morphine, once daily for tree days and then five days free of the drug before test. Results. Our results showed bilateral intra-VHC administration of histamine (2.5, 5 and 7.5μg/rat) decreased %OAT (Open Arm Times) and %OAE (Open Arm Entries) but not locomotor activity that showing an anxiogenic effect. Another results indicated that morphine sensitization increased %OAT and %OAE. Conclusions. Bilateral intra-VHC administration of histamine showed an anxiogenic effect. Moreover, another results indicated that morphine sensitization indicated an anxiolytic response in the presence of histamine. histamine anxiety sensitization plus- maze rat 2011 1 01 53 58 http://ncmbjpiau.ir/article-1-10-en.PDF
3-11 2024-03-28 10.1002
New Cellular and Molecular Biotechnology Journal NCMBJ 2228-5458 2228-6926 - 2011 1 1 Isolation of Mycobacterium Chelonae in the Sputum of Patient with Metastatic Breast Cancer Saeed Zaker Bostanabad Pirouz salehian Mostafa Ghalami Shahin Pourazar Dizaji  Aim and Background.Mycobacterium chelonae is non-tuberculous mycobacteria (NTM), a grouping that encompasses all mycob acteria outside of the Mycobacterium tuberculosis complex. M. chelonae causes various clinical syndromes, including lung disease, local cutaneous disease, osteomyelitis, joint infections, and ocular disease. With the exception of lung disease, these syndromes co mmonly may be seen after trauma. Mycobacterium chelonae is a rare cause of isolated lymphadenitis. Endocarditis has also been documented. Disseminated skin and soft tissue disor ders & almost exclusively in the setting of immuno-suppression, especially AIDS. Esophageal disorders increase the risk for pulmonary disease due to rapidly growing mycobacteria. Surgical-wound infections due to Mycobacterium chelonae are well documented, especially in association with cardiothoracic surgery and augmentation mammoplasty. Alternative practices such as mesotherapy have been associated with skin infections. No human-to-human transmission has been documented. Materials and Methods. Sputum was collected from suspected non-tuberculosis patient case had proven registration of clinical diagnostic examination. This isolate was cultured on Lowenstein Jensen solid medium and grown colonies after 7 days. All identification testing and drug susceptibility testing was done accordance CDC standard method. PCR amplification with several primers for identifying complex tuberculosis from Chelonae was performed. Results. Acid fast staining, uramin and culture tests has been positive. Tuberculin, PCR amplifications has been negative. Growth on 2- thiphene carboxylic acid medium was positive in Lowenstein Jensen and colonies of bacteria was non- photochromogen. Catalase test in two temperatures has been positive and niacin- nitrate tests was identified negative. Conclusions. In this study, this isolates was from mycobacterium chelonae complex that has been isolated from patient with metastatic breast cancer and virulence of Mycobacterium chelonae is in the human with other problem consist of cancer, autoimmune, Allergy, osteomyelitis and etc. Mycobacterium Chelonae Metastatic Breast Cancer 2011 1 01 59 63 http://ncmbjpiau.ir/article-1-11-en.PDF