2024-03-28T17:59:37+03:30 http://ncmbjpiau.ir/browse.php?mag_id=31&slc_lang=fa&sid=1
31-1053 2024-03-28 10.1002
New Cellular and Molecular Biotechnology Journal NCMBJ 2228-5458 2228-6926 - 2018 8 29 The roles of HMGB1 nuclear protein and its major domains in different biological fields Somayeh Talebi Azam Bolhassani azam.bolhassani@yahoo.com Talat Mokhtari Azad Arash Arashkia Mohammad Hossein Modarressi HMGB1 protein is an intracellular protein that translocated into the nucleus and regulates genes expression. HMGB1 plays multi-functional roles through recombination, transcriptional regulation, and inflammation. HMGB1 binds to cyclin-dependent kinase such as CDK2 that regulates the transcription of genes associated with the progression of cell cycle. In addition, HMGB1 as a novel cytokine plays major role in inflammation and arthritis. Recently, the role of HMGB1 protein as an adjuvant and stimulating both humoral and cellular immune responses has been proven in viral infections such as HIV-1 and influenza. Several studies indicated that Hp91, a HMGB1-derived peptide, stimulates efficiently DC maturation, and boosts the cellular and humoral immune responses in vivo. Our recent data also showed that the full length of HMGB1 gene, and Hp91 peptide could be an effective adjuvant in developing the therapeutic HPV DNA- and protein-based vaccines, respectively. In this review, the structure and functions of HMGB1 is described in molecular biology and medicine. Endogenous adjuvant HMGB1 Hp91 Biological activity 2018 1 01 9 22 http://ncmbjpiau.ir/article-1-1053-en.pdf
31-1054 2024-03-28 10.1002
New Cellular and Molecular Biotechnology Journal NCMBJ 2228-5458 2228-6926 - 2018 8 29 Investigation of relationship between MicroRNA Machinery Genes polymorphism(RAN rs14035,DICER rs3742330 )and recurrent spontaneous abortion in city of Tehran Amir Fallah-Sohy Saeed Zaker Bostanabad saeedzaker20@yahoo.com Sina Mirza ahmadi aim and Background: Recurrent spontaneous abortion is defined as the occurrence of at least three consecutive pregnancy losses prior to 20th week of gestation.Key molecules like Ran and Dicer involves in microRNA (miRNA) biogenesis,miRNAs are key regulators in many biological processes.The purpose of this study was to demonstrate whether genetic polymorphisms in miRNA machinery genes(RAN rs14035,DICER rs3742330) show an association with recurrent spontaneous abortion in woman of the city of Tehran. Materials and Methods: one case-control study was performed with 20 healthy women (with at least one live birth and no history of pregnancy loss) and 50  patient women with  recurrent spontaneous abortion for genotyping of DICER polymorphism by tetra ARMS PCR method, another study was performed with 10 healthy women(with at least one live birth and no history of pregnancy loss)  and 40 patient women with  recurrent spontaneous abortion for genotyping of RAN polymorphism by PCR and sequencing method.Statistical data of the two polymorphism were analyzed by Chi-square and Fisher's exact test. Results: Genotypes and alleles  of DICER(rs3742330) polymorphism showed no significant association with recurrent spontaneous abortion in the studied  group (p>0/05).Genotypes of RAN(rs14035) polymorphism showed a significant association with recurrent spontaneous abortion in the studied  group(p=0/001),but there was no significant association between this condition and RAN polymorphism in the studied group(p>0/05). Conclusion: This study indicates that RAN(rs14035) has significant association with recurrent spontaneous abortion but this condition has no  significant association with DICER(rs3742330) .   miRNA Machinery Genes DICER RAN Tetra Arms PCR Single nucleotide polymorphism Recurrent spontaneous abortion Sequencing 2018 1 01 23 30 http://ncmbjpiau.ir/article-1-1054-en.pdf
31-1055 2024-03-28 10.1002
New Cellular and Molecular Biotechnology Journal NCMBJ 2228-5458 2228-6926 - 2018 8 29 Assessment of virulence factors(elastase and phospholipase) and antibiotic susceptibility in Pseudomonas aeruginosa isolated from clinical samples Mahya rahbar zare Fatemeh Noorbakhsh niloofar_noorbakhsh@yahoo.com Sahar Honarmand jahromi Aim and Background:  Pseudomonas aeruginosa is one of the most important pathogen cause infection in hospital in world. Different virulence gene exsist in this bacteria, which interfere in pathogenesis and infection.  The aim of this study was evaluation of antibiotic susceptibility pattern and identify virulence genes plcH, lasB, plcN of P. aeruginosa isolated from clinical samples.   Material and methods:  In this study 100 Pseudomonas aeruginosa isolated of human cases of burn in 1395. Antibiotic susceptibility test was performed by disk diffusion method. Genomic DNA were extracted from isolated strains and Multiplex- PCR performed to determine the preavalence  of each of the tested genes. Result: The scope age of  patients were 20-90 years, and 46 patient were male and 52 were female.The prevalence of plcN and plcH  observed  98 percent and lasB 100 percent.  The highest resistance to antibiotics were observed in  ampicillin and cefoxitin with the frequency of  93% and 96% respectively. Conclusion:The  high prevalence of plcH, lasB, plcN gene in Pseudomonas aeruginosa isolated of skin case of burn revealed that this genes are an important virulence factors in burn infections. Pseudomonas aeruginosa plcH lasB plcN 2018 1 01 31 38 http://ncmbjpiau.ir/article-1-1055-en.pdf
31-1056 2024-03-28 10.1002
New Cellular and Molecular Biotechnology Journal NCMBJ 2228-5458 2228-6926 - 2018 8 29 Reconstruction of Gene Networks Involved in Response to Drought Stress in Barley Seyede Mehri Javadi Zahra-Sadat Shobbar shobbar@abrii.ac.ir Asa Ebrahimi Maryam Shahbazi Aim and Background: Considering the importance of drought stress, and the fact that drought tolerance is a multigenic trait, the objective of the current research was reconstruction of the related gene network and identification of the involved key genes in barley, through microarray data analysis as well as gene ontology enrichment test. Materials and Methods: To achieve this goal, all the differentially expressed genes (-2.5 ≤Fold change≤ 2.5) at drought stress conditions among the available barley microarray datasets were identified using Genevestigator web-based tools. Reconstruction of protein–protein interactions (PPIs) network and hub gene analysis were accomplished using Cytoscape software. Gene ontology enrichment analyses were done by AgriGo tools. Results: Hub gene analysis led to the identification of 14 and 15 unique genes in vegetative and reproductive stages, respectively, which were considered as the most effective genes in response to drought stress. Conclusion: Based on the achieved results, ARF1, ABF3, DREB1A, DREB2A, HY5, and HYH can be introduced as the most effective key genes involved in drought stress tolerance. Moreover, gene ontology analysis of the differentially expressed and hub genes demonstrated transcriptional regulation, processes of protein metabolism, photoperiod control, photosynthesis and hormonal signaling by protein kinases and also flowering time control play vital a role in the major and effective processes involved in drought tolerance. Barley Drought stress Biological network Hub genes 2018 1 01 39 48 http://ncmbjpiau.ir/article-1-1056-en.pdf
31-1057 2024-03-28 10.1002
New Cellular and Molecular Biotechnology Journal NCMBJ 2228-5458 2228-6926 - 2018 8 29 Biological composting in some of the agricultural organic waste and survey of microorganism kinetic models Fathollah Gholami-Borujeni Fatemeh Nejatzadeh fnejatzadeh@yahoo.com Aim and Background: This study aimed to determine the aeration rate and its reaction kinetics in aerobic composting of some agricultural wastes. Material and methods: For this aim compost materials were prepared by mixing grass trimmings, tomato, pepper, and eggplant wastes. Four vertical forced aeration type reactors and one vertical natural convection type reactor were manufactured to apply four different aeration rates. CO2 rate and temperature changes were recorded in three different places in the reactors. Moisture content, pH and organic material rate were recorded each day. While process-monitoring parameters (CO2, temperature, pH, moisture content) were used for interpretation of the process, organic material degradation was used for interpretation of the process success. The seven different kinetic models were applied for modeling biological decomposition rate to the experimental values. Results: According to the results, four of these models were found applicable to this study. These models were analyzed with some statistical methods as root mean square error (RMSE), chi-square (χ2), and modeling efficiency (EF). According to the statistical results of these models, the best model was found as: where kT is the rate of decomposition (g VS/g VS day); T the process temperature (0C); Mc the daily moisture content (%wb); C the daily CO2 rate in composting reactor (%) and a, b, c, d are constants. Conclusion: According to the results, the highest organic matter degradation and temperature value were obtained at the aeration rate of 0.4 l air min1 kg1 om. Thus, it could be applied to this mixed materials composting process. Compost Agricultural waste Aeration Rate Kinetic Microorganism 2018 1 01 49 56 http://ncmbjpiau.ir/article-1-1057-en.pdf
31-1058 2024-03-28 10.1002
New Cellular and Molecular Biotechnology Journal NCMBJ 2228-5458 2228-6926 - 2018 8 29 Effect of Azospirillium on the morphological and physiological traits of basil (Ocimum basilicum) under salt stress. Hadi Mohammadi Babazeidi mohammadihadi45@yahoo.com Ali Hatami Mehrshad Barari Mohammad Javad Zare Mehran Falaknaz Aim and Background: Salinity is one of the main factors limiting the production of crops. On other hand, reducing rainfall and increasing excessive heat in the past few years has led to more evapotranspiration and increased salinity effects in reducing yields. Increasing the consumption and economic value of medicinal plants, including Basil, is essential for research on the limiting factors in planting these plants in the country. Due to the contamination caused by chemical fertilizers and the high energy consumption for their production, bio-fertilizers can be a good alternative to chemical fertilizers. The present study deals with the effect of Azospirillium bacteria on physiological and morphological characteristics of Basil's medicinal plant under salinity stress Material and methods: This experiment was conducted in May 2011 in Greenhouse of College of Agriculture in the University of Ilam. The experiment was factorial based on randomized complete block design with 3 replications and 4 salinity levels of 2,4,6 and 0 (ds / m) and 2 levels of presence and absence of bacteria Results: The results of this study showed that with increasing levels of stress, most of the traits are affected. The plant was lower at lower levels, but at 4 dS / m and 6 dS / m levels, the yield was significantly reduced. Increasing levels of stress reduced the height of root dry weight, root length and biomass weight, and also it reduced the number of leaves, sub branch and chlorophyll a, b and ab. Also, salt stress increased levels of nitrogen, protein and proline by increasing salinity levels. Salinity stress Azospirillium Basil Physiological and Morphological Characteristics 2018 1 01 58 68 http://ncmbjpiau.ir/article-1-1058-en.pdf
31-1059 2024-03-28 10.1002
New Cellular and Molecular Biotechnology Journal NCMBJ 2228-5458 2228-6926 - 2018 8 29 Investigation of drug resistance against treatment with Enzalutamide medicine in individuals diagnosed with prostate cancer and studying the effect of rs137852574 single-nucleotide polymorphism in drug resistance in the human population of Isfahan province Bita Kaviani Hosein Sazgar hoseinsazgar@yahoo.com Nusha Zia Jahromi Farzane Mohamadi Farsani Aim and Background: The purpose of this study is to investigate role of rs137852574 single-nucleotide polymorphism in the androgen receptor coding gene on drug resistance of patients with prostate cancer against Enzalutamide. Material and methods: In this study, the ARMS-PCR analysis was conducted on androgen receptor coding gene in 50 patients with prostate cancer with drug resistance and 50 patients with prostate cancer without drug resistance. Blood samples were collected from patients admitted to Omid and al-Zahra hospitals of Isfahan province. Results: The allele frequencies of T and G alleles in rs137852574 were 0.72 and 0.28 for drug resistant and 0.88 and 0.12 for non-drug resistance groups. In addition, Evaluation of heterozygosity showed that the marker was in Hardy-Weinberg equilibrium. Conclusion: The results indicate that there is a meaningful relationship between drug resistance and rs137852574 single-nucleotide polymorphism existence (P-Value = 0.039). Moreover, results gained from investigating the drug resistance mechanism using the docking technique showed that in individuals with rs137852574 single-nucleotide polymorphism, the drug is not accurately bonded to the active pocket of androgen receptor, and consequently this drug’s binding energy to the receptor is reduced. Drug resistance Prostate cancer Single nucleotide polymorphism docking. 2018 1 01 69 78 http://ncmbjpiau.ir/article-1-1059-en.pdf
31-1062 2024-03-28 10.1002
New Cellular and Molecular Biotechnology Journal NCMBJ 2228-5458 2228-6926 - 2018 8 29 Investigation of microbial quantity of indoor and outdoor air of selected daycare centers in different districts and seasons of Ahvaz Atefeh Harbizadeh harbizade.a@gmail.com Gholamreza Goudarzi farkhondeh Salehshoshtari Aim and Background: Air pollution is considered as one of the major causes of environmental health problems. Young children are more susceptible than adults due to age and higher sensitivity health hazards. The aim of study was to investigation indoor/outdoor, regional and seasonal variations of bio-aerosol emission of selected child daycare centers (sites) in Ahvaz city. Materials & Methods: bio-aerosol samplings were performed bimonthly at indoor and outdoor of six sites at different region (industrial, residential, traffic) in a period of 8/12/2015 to 24/5/2016 and three seasons (autumn, winter and spring) in Ahvaz city. Air samplings were conducted by quick take. The flow rate and height were 28.3 lit/min and 0.8-1m above ground level respectively. Collected samples were immediately transferred in the cool-box to laboratory for further experiments. Tryptic soy agar (TSA) was used as nutrient media for the bacterial growth. After incubation, colonies on each plate were counted and the concentrations were calculated as colony forming units per cubic meter of air (CFU/m3). Genera identification of cultured airborne bacteria was conducted by examination of gram-stained smears and standard biochemical tests. It should be noted that due to the high number of isolates, a number of different colonies were selected based on the results of microscopic examination and also characteristics such as colony size, colony appearance and possible pigment from each plate.During sampling, indoor and outdoor temperature and relative humidity were measured. Results: The results shows that I/O rate of microbial populations at indoor was more than that of the outdoor. The most common bacterial groups found in outdoor and indoor air of the all sites were Gram-positive bacilli and cocci respectively. The highest and lowest concentrations of bacteria were observed in February and November respectively. The dominant bacterial genera were Staphylococcus spp. and Bacillus spp. Conclusion: We can conclude that the highest and lowest mean concentrations of bacteria were observed at sites located in high traffic, crowded areas and residential and low traffic region respectively. There is no significant relationship between concentration of bacteria and temperature in the sampling sites. Air quality Bio-aerosols Child day-care Centers 2018 1 01 78 96 http://ncmbjpiau.ir/article-1-1062-en.pdf
31-1061 2024-03-28 10.1002
New Cellular and Molecular Biotechnology Journal NCMBJ 2228-5458 2228-6926 - 2018 8 29 The Investigation of Organic and Chemical Fertilizers Effects on the Yield and Phenolic Compounds Extract of (Rosa damascene Mill) Reza Dehghani Bidgoli dehghanir@kashanu.ac.ir Zahra Abdollahpoor Maryam Akhbari Aim and Background: Rose scientific name is Rosa damascene Mill. The aim of this study was to evaluate the quality and quantity of phenolic compounds in this plant that treated by both manure and chemical fertilizers. Material and methods: After appropriate treatments (manure and chemical fertilizers) at 2 habitats and 3 replication extraction conducted  by soaking in 70% ethanol. Then measurement of phenolic and flavonoids compounds has been done by spectrophotometry method. Results: The results of phytochemical tests confirmed the presence of tannin, anthocyanin and flavonoids and absence of alkaloids in all treatments. Chemical fertilizer increased the flavonoids compounds manure fertilizer increased phenolic compounds Conclusion: Results indicated that the efficiency of different treatments can effect of plant’s extract efficiency and phenolic compounds that So far for this plant species has not been reported in the scientific literature. phenol spectrophotometry manure Extract Rosa damascene Mill 2018 1 01 79 86 http://ncmbjpiau.ir/article-1-1061-en.pdf
31-1063 2024-03-28 10.1002
New Cellular and Molecular Biotechnology Journal NCMBJ 2228-5458 2228-6926 - 2018 8 29 Isolation of bla_vim gene in Antibiotic resistant Pseudomonas aeruginosa from hospitals Elham Siasi emi_biotech2006@yahoo.ca Robab Rafiei Tabatabaii Fatemeh MoslehiMehr Aim and Background: Overplay of antibiotics in clinical centers not only did not destroy pathogenic bacterias but also grew a resistance for these kind of compounds in microorganisms such as Pseudomonas aeruginosa that is one of important infection factors in hospitals. The purpose of this study is to investigate the resistant gene to antibiotic, bla_vim gene, which was found among Pseudomonas aeruginosas released from hospitals. Materials and Methods: 120 strains of Pseudomonas aeruginosas were collected from hospitals. The sensitivity of these strains to antibiotics was determined by Disc diffusion test. PCR was used for identification of bla_vim gene . Results: Among 120 samples isolated from hospitals 50 cases were Pseudomonas aeruginosa. 35 Pseudomonas aeruginosa samples were resistant to antibiotics. 21 samples (42%) of 35 samples contained bla_vim gene. Conclusion: The results of this study indicated the presence of bla _vim gene in Pseudomonas aeruginosa. The comparison of the results with the rest of the world indicates the increasing genetic resistance of Pseudomonas aeruginosa to antibiotics especially beta-lactams. These results are important in taking proper health policies and using effective antibiotics for hospital infections. Pseudomonas aeruginosa antibiotic resistance bla _vim gene beta-lactams 2018 1 01 97 106 http://ncmbjpiau.ir/article-1-1063-en.pdf
31-1064 2024-03-28 10.1002
New Cellular and Molecular Biotechnology Journal NCMBJ 2228-5458 2228-6926 - 2018 8 29 Incidence of new bacterial species resistance to copper and antibiotics in smeltery industrial wastewater in Isfahan Zahra Amirpoor Monir Doudi GHolam Reza Amiri Aim and Background:Identification of strains resistant to the toxic metal is an effective step in the process of bioremidation. This study aimed to determine the minimum inhibitory concentration (MIC)  of bacteria resistant to copper and some antibiotics bacteria isolated from copper  smiths’ workshop in Isfahan. Materials and Methods:In this study, the minimum inhibitory concentration (MIC) of bacteria was determined against copper and then the final identification colony- PCR was performed using the technique and evaluted copper -resistant bacteria and resistance against several . :Among the resistant bacteria isolated highest MIC (2 mM) of the copper from waste copper were determined. Resistant bacteria including antibiotic-resistant strains of  Stenotrophomonas maltophilia, which was than most. : Due to the large number of bacteria resistant to antibiotics and resistant bacteria at the same time as the copper, unfortunately, these bacteria can in the future be used for the biosorption of copper from the environment unless they removed the gene for antibiotic resistance. Resistant bacteria Copper Antibiotics Colony PCR MIC 2018 1 01 107 116 http://ncmbjpiau.ir/article-1-1064-en.pdf
31-1065 2024-03-28 10.1002
New Cellular and Molecular Biotechnology Journal NCMBJ 2228-5458 2228-6926 - 2018 8 29 Study of Immobilization of Cytochrome c on the Modified Gold and GraphiteElectrode by Electrochemical Method Mahnaz Moradi Hamid Zare-zade Mehrizi Ali Naderinezhad Bibi Fateme Haghirosadat fhaghirosadat@ut.ac.ir Mohammad Kazem Hazrati Gohari Fateme Daneshmand Fatemeh Hakimian Azim Akbarzade Aim and Background: Immobilized proteins and enzymes are commonly used in the medical field for diagnosis and treatment of various diseases. Due to the increasing use of immobilized enzymes and proteins in the diagnosis of disease, in this study, Cytochrome c protein was used as a useful metalloprotein in the basis studies of biosensors and Bioelectrochemistry. Material and methods: Cytochrome c protein immobilized covalently on the surface of gold and graphite pencil electrods. Surface of gold and graphite electrodes modified by gold nanoparticle and self-assemble monolayer of 3- mercaptopropionic acid. EDC and NHS as coupling agents cause to covalent attachment of protein on the surface. Results: Cyclic voltammetry results showed successful immobilization of Cytochrome c on the both surface. Immobilized protein on the surface of gold electrod was more stable than garaphite electrode. Conclusion: In view of the Cytochrome c as a sample biological species, both surfaces can be suitable for immobilization of various biological species, and selection any of them depend on their application in various fields. Immobilization Cytochrome C Gold nanoparticle Cyclic Voltammetry 2018 1 01 117 125 http://ncmbjpiau.ir/article-1-1065-en.pdf