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:: Volume 7, Issue 28 (10-2017) ::
NCMBJ 2017, 7(28): 33-41 Back to browse issues page
Effectiveness of Malva sylvestris extract on growth and biofilm formation of Pseudomonas aeruginosa
Sargol Aminnezhad , Roha Kasra kermanshahi , Morteza Mirzai , Jamal Rashidiani
Department of Biology, Faculty of Basic Sciences, Alzahra University, Tehran, Iran , rkasra@yahoo.com
Abstract:   (2881 Views)
Aim and Background:
Pseudomonas aeruginosa is an important opportunistic pathogen that typically makes a biofilm. P. aeruginosa biofilms are intrinsically resistant to antimicrobial chemotherapies.The aim of this study was to evaluate the inhibitory effects of ethanolic extract of Malva sylvestris on the biofilm formation of Pseudomonas aeruginosa ATCC:27853 .
Material and methods:
The alcoholic extracts of Malva sylvestris which were collected from north western Iran(Kurdistan)were obtained by soxhlet apparatus. The antibacterial effect of plant extract was determined by well diffusion agar. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were assessed  by micro dilution method according to CLSL standard.
Finally biofilm formation of these this strain in presence of ethanolic extract of M. sylvestris was determined using modified micro titer plate method. Statistic analysis was done by  t-test  and  analysis  of  variance(ANOVA)  was  used  to  compare  means  and  data correlation.
The results showed a significant effect of ethanolic extract of M. sylvestris on the tested strain. The MIC and MBC were 62.5 mg ⁄ ml. The different concentration of ethanolic extract of  M. sylvestris  decreased significantly in biofilm formation. on P.aeruginosa in compared to gentamicin as the positive control (P value = 0.0124).
Conclusion :
According to the finding Malva sylvestris, is effective in killing P. aeruginosa. bacterial biofim  so  this medicinal plant  appears to be promising agent for prophylaxis against various pseudomonas infections
Keywords: Pseudomonas aeruginosa, MIC, MBC, biofilm, Malva sylvestris
Full-Text [PDF 405 kb]   (890 Downloads)    
Type of Study: Research Article | Subject: Cellular and molecular
Received: 2017/12/30 | Accepted: 2017/12/30 | Published: 2017/12/30
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aminnezhad S, kasra kermanshahi R, mirzai M, rashidiani J. Effectiveness of Malva sylvestris extract on growth and biofilm formation of Pseudomonas aeruginosa. NCMBJ. 2017; 7 (28) :33-41
URL: http://ncmbjpiau.ir/article-1-1030-en.html

Volume 7, Issue 28 (10-2017) Back to browse issues page
مجله تازه های بیوتکنولوژی سلولی - مولکولی New Cellular and Molecular Biotechnology Journal
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