Volume 8, Issue 29 (1-2018)
NCMBJ 2018, 8(29): 97-106 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Siasi E, Rafiei Tabatabaii R, MoslehiMehr F. Isolation of bla_vim gene in Antibiotic resistant Pseudomonas aeruginosa from hospitals. NCMBJ 2018; 8 (29) :97-106
URL: http://ncmbjpiau.ir/article-1-1063-en.html
URL: http://ncmbjpiau.ir/article-1-1063-en.html
Department of Microbiology, School of science, Islamic Azad University, North Tehran Branch, Tehran, Iran , emi_biotech2006@yahoo.ca
Abstract: (4497 Views)
Aim and Background: Overplay of antibiotics in clinical centers not only did not destroy pathogenic bacterias but also grew a resistance for these kind of compounds in microorganisms such as Pseudomonas aeruginosa that is one of important infection factors in hospitals. The purpose of this study is to investigate the resistant gene to antibiotic, bla_vim gene, which was found among Pseudomonas aeruginosas released from hospitals.
Materials and Methods: 120 strains of Pseudomonas aeruginosas were collected from hospitals. The sensitivity of these strains to antibiotics was determined by Disc diffusion test. PCR was used for identification of bla_vim gene .
Results: Among 120 samples isolated from hospitals 50 cases were Pseudomonas aeruginosa. 35 Pseudomonas aeruginosa samples were resistant to antibiotics. 21 samples (42%) of 35 samples contained bla_vim gene.
Conclusion: The results of this study indicated the presence of bla _vim gene in Pseudomonas aeruginosa. The comparison of the results with the rest of the world indicates the increasing genetic resistance of Pseudomonas aeruginosa to antibiotics especially beta-lactams. These results are important in taking proper health policies and using effective antibiotics for hospital infections.
Materials and Methods: 120 strains of Pseudomonas aeruginosas were collected from hospitals. The sensitivity of these strains to antibiotics was determined by Disc diffusion test. PCR was used for identification of bla_vim gene .
Results: Among 120 samples isolated from hospitals 50 cases were Pseudomonas aeruginosa. 35 Pseudomonas aeruginosa samples were resistant to antibiotics. 21 samples (42%) of 35 samples contained bla_vim gene.
Conclusion: The results of this study indicated the presence of bla _vim gene in Pseudomonas aeruginosa. The comparison of the results with the rest of the world indicates the increasing genetic resistance of Pseudomonas aeruginosa to antibiotics especially beta-lactams. These results are important in taking proper health policies and using effective antibiotics for hospital infections.
Type of Study: Research Article |
Subject:
Microbiology
Received: 2018/02/28 | Accepted: 2018/02/28 | Published: 2018/02/28
Received: 2018/02/28 | Accepted: 2018/02/28 | Published: 2018/02/28
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
