Volume 8, Issue 31 (7-2018)
NCMBJ 2018, 8(31): 65-74 |
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Ghafari O, Foroohi F, Nemati F. Review and compare Mycobacterium paratuberculosis isolated from livestock in the provinces of Tehran and Alborz by PCR, SSR methods. NCMBJ 2018; 8 (31) :65-74
URL: http://ncmbjpiau.ir/article-1-1126-en.html
URL: http://ncmbjpiau.ir/article-1-1126-en.html
Department of Microbiology, Shahr-e- Quds Branch, Islamic Azad University, Tehran, Iran , f_foroohi@ymail.com
Abstract: (5105 Views)
Aim and Background:
Mycobacterium avium subspecies paratuberculosis (MAP) is causative agent of Johne's disease. The disease is worldwide spread and causes important economic losses in livestock industries.
Materials and Methods:
This study was performed on 30 bacterial isolates that different by geographical area (provinces of Tehran and Alborez) and host (cattle, sheep and goat). PCR method was used for amplification of two locos SSR1 and SSR2.
Results:
The results showed that isolates have different structurally based on SSR1and SSR2 each other and also with the standard strain. The locus of Insertion and Deletion due to repetitive sequences in tandem, different genetic patterns observed that the ability to distinguish between strains provides.
Conclusion:
Considering the two alleles of each gene, genetic samples were placed in two types.
Mycobacterium avium subspecies paratuberculosis (MAP) is causative agent of Johne's disease. The disease is worldwide spread and causes important economic losses in livestock industries.
Materials and Methods:
This study was performed on 30 bacterial isolates that different by geographical area (provinces of Tehran and Alborez) and host (cattle, sheep and goat). PCR method was used for amplification of two locos SSR1 and SSR2.
Results:
The results showed that isolates have different structurally based on SSR1and SSR2 each other and also with the standard strain. The locus of Insertion and Deletion due to repetitive sequences in tandem, different genetic patterns observed that the ability to distinguish between strains provides.
Conclusion:
Considering the two alleles of each gene, genetic samples were placed in two types.
Type of Study: Research Article |
Subject:
Cellular and molecular
Received: 2018/09/5 | Accepted: 2018/09/5 | Published: 2018/09/5
Received: 2018/09/5 | Accepted: 2018/09/5 | Published: 2018/09/5
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