Volume 8, Issue 31 (7-2018)
NCMBJ 2018, 8(31): 29-38 |
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Noormohamadi E, Farahmand M, Esmaeili Rastaghi A R. Identification of extermophilies producing l- glutaminase and l-asparaginase from Larijanhotspring. NCMBJ 2018; 8 (31) :29-38
URL: http://ncmbjpiau.ir/article-1-1131-en.html
URL: http://ncmbjpiau.ir/article-1-1131-en.html
Biology department, East Tehran Branch , Islamic Azad University ,Tehran, Iran , farahmand90@yahoo.com
Abstract: (5638 Views)
Background and Objective:
The most frequently used sources of more stable enzymes are thermophilic bacteria. In recent years microbial L-asparaginase has been widely used as therapeutic agent in the treatment of human cancers. Microbial L-glutaminase is currently used in the treatment of acute lymphoblastic leukemia (ALL) and human immunodeficiency virus (HIV) because of its potential applications in medicine . The aim of present study was to screen new potent thermophilic bacterial isolates producing L-asparaginase and L-glutaminase.
Methods:
Larijan hotspring was chosen as the location for isolation of the thermophilic bacteria. Culture and morphological and biochemical tests, were performed. All the bacterial isolates were screened in ortherto L-asparaginase and L-glutaminase enzyme activity using Rapid Plate assay method. M9 modified media incorporated L-asparaginase or L-glutaminase and phenol red as indicator were used. Thermophilic strains were later identified by 16SrRNA gene ribotyping followed by PCR .
Results:
A total of six isolates recovered from Larijan hotspring were found to belong to Bacillus licheniformis. The L-asparaginase activity was observed in LT-1, LT-2, LT-3, LT-4, LT-5 strains.The L-glutaminase produced by Bacillus licheniformis LT-1, LT-3, LT-5 and LT-6 strains. Three thermophilic LT-1, LT-3, LT-5 strains with L-glutaminase and L-asparaginase activity have been successfully isolated.
Conclusion:
Larijan hot spring is one of the potential sources of useful microbial products that can be used as a source of useful different biological products like enzymes.
The most frequently used sources of more stable enzymes are thermophilic bacteria. In recent years microbial L-asparaginase has been widely used as therapeutic agent in the treatment of human cancers. Microbial L-glutaminase is currently used in the treatment of acute lymphoblastic leukemia (ALL) and human immunodeficiency virus (HIV) because of its potential applications in medicine . The aim of present study was to screen new potent thermophilic bacterial isolates producing L-asparaginase and L-glutaminase.
Methods:
Larijan hotspring was chosen as the location for isolation of the thermophilic bacteria. Culture and morphological and biochemical tests, were performed. All the bacterial isolates were screened in ortherto L-asparaginase and L-glutaminase enzyme activity using Rapid Plate assay method. M9 modified media incorporated L-asparaginase or L-glutaminase and phenol red as indicator were used. Thermophilic strains were later identified by 16SrRNA gene ribotyping followed by PCR .
Results:
A total of six isolates recovered from Larijan hotspring were found to belong to Bacillus licheniformis. The L-asparaginase activity was observed in LT-1, LT-2, LT-3, LT-4, LT-5 strains.The L-glutaminase produced by Bacillus licheniformis LT-1, LT-3, LT-5 and LT-6 strains. Three thermophilic LT-1, LT-3, LT-5 strains with L-glutaminase and L-asparaginase activity have been successfully isolated.
Conclusion:
Larijan hot spring is one of the potential sources of useful microbial products that can be used as a source of useful different biological products like enzymes.
Type of Study: Research Article |
Subject:
Cellular and molecular
Received: 2018/09/8 | Accepted: 2018/09/8 | Published: 2018/09/8
Received: 2018/09/8 | Accepted: 2018/09/8 | Published: 2018/09/8
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