Aim and Background. The Juglans regia L. is a plant with difficulty in rooting, therefore, its asexual reproduction is almost impossible. The aim of this study was to conduct and improve the advantage of asexual reproduction by using growth regulators of  IBA and 2,4-D.

Materials and Methods. In order to keep major characteristics, we studied the effects of treatments and growth regulators of  IBA and 2,4-D in several concentrations on 40 plantlet in five groups.

Results. The results showed that application of IBA has important  effects on root primordium initiating  but application of 2,4-D does not show significant initiation. Both treatments of IBA and 2,4-D tend to increasing length of roots.

Conclusion. This research show that,  treating of shoots with IBA  have not important effect on shoot primordium initiation,  but  2,4-D treatment are very important effects. Over all treatment with IBA and 2,4 –D caused to increasing the rooting  in survival of cutting.