Volume 10, Issue 39 (6-2020)                   NCMBJ 2020, 10(39): 39-48 | Back to browse issues page

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Heidari F, Doosti A. Evaluation of GPR83 Gene Expression in AGS Cells Transfected with Pflag-CMV-3-TagD Recombinant Vector. NCMBJ 2020; 10 (39) :39-48
URL: http://ncmbjpiau.ir/article-1-1298-en.html
Department of Biology, Faculty of Science, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
Abstract:   (2356 Views)
Aim and Background: Helicobacter pylori infection is a major cause of gastric cancer in humans. The Helicobacter pylori tagD gene, which encodes the thiol peroxidase enzyme, plays an important role in bacterial colonization in the human stomach wall. Research has shown that the GPR83 gene in gastric cancer increases expression, and the aim of this study was to investigate the expression of the GPR83 gene in AGS cells transfected with the recombinant pFLAG-CMV-3-tagD vector by Real Time PCR.

Materials and methods: AGS cells were transfused using lipofactamine solution and plasmid carrying the tagD gene encoding Helicobacter pylori or empty plasmid (control). RNA extraction was then performed from cultured cells and cDNA synthesis was performed, and then the eukaryotic expression of Helicobacter pylori gene tagD in AGS cells was investigated by RT-PCR method. The expression of GPR83 genes was evaluated by Real Time PCR method. It should be noted that the enoxin kit was used to evaluate apoptosis, and finally the expression of each gene was evaluated using SPSS software and t-test Indepent statistical tests.
Results: Results: The findings from gene expression analysis showed that the expression of GPR83 gene in AGS cells treated with tagD increased compared to control cells, but this increase in expression was not statistically significant (P = 0.0888).
Conclusion: Overall, the data obtained from this study showed that GPR83 gene expression is altered in cells treated with the Helicobacter pylori tagD gene and seems to play a role in the expression of Helicobacter pylori
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Type of Study: Research Article | Subject: Cellular and molecular
Received: 2020/08/2 | Accepted: 2020/03/29 | Published: 2020/03/29

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