Volume 11, Issue 44 (9-2021)
NCMBJ 2021, 11(44): 41-56 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Ghorbani Moghaddam R, Asgharzadeh A, Amani A, Hosseinzadeh M R, Kasaian J. Evaluation of antibacterial effect of methanolic extract of Verbascum thapsus L. Ecotypes in North Khorasan against of Escherichia coli, Bacillus cereus and Staphylococcus aureus in vitro. NCMBJ 2021; 11 (44) :41-56
URL: http://ncmbjpiau.ir/article-1-1425-en.html
URL: http://ncmbjpiau.ir/article-1-1425-en.html
Raheleh Ghorbani Moghaddam 
, Ahmad Asgharzadeh , Amir Amani , Mohammad Reza Hosseinzadeh , Jamal Kasaian
, Ahmad Asgharzadeh , Amir Amani , Mohammad Reza Hosseinzadeh , Jamal Kasaian
Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran
Abstract: (2263 Views)
Abstract
Aim and Background:Wild plants in nature are valuable samples for use in various studies due to their characteristics such as resistance to adverse environmental conditions, resistance to pests and diseases and even having high active ingredients. Today, with the increasing resistance of bacteria to antibiotics, the use of medicinal plants has been welcomed.The present study aimed to investigate the effect of antibacterial effects of different ecotypes of Verbascum thapsus extract.
Materials and Methods:Verbascum thapsus from three regions of North Khorasan (Ashkhaneh, Bojnourd and Shirvan), after collection and drying, was extracted by methanol solvent by soaking and its antibacterial properties was investigation against( Escherichia coli, Bacillus cereus and Staphylococcus aureus) using disk and well diffusion , MIC (Minimum Inhibitory Concentration ) and MBC ( Minimum Bactericidal Concenteration) methods.
Results:The results showed that between different ecotypes of North Khorasan, Verbascom extract of Bojnourd region had the most inhition zone in disk and well diffusion method on Staphylococcus aureus bacteria were equal to 12.33 and 14.66 mm, respectively, and for Bacillus cereus bacteria were 11.33 and 13.66 mm, respectively And MIC and MBC of Verbascum extract of Bojnourd region on bacteria (Staphylococcus aureus) 12. mg/ml were observed as the most sensitive bacteria.
conclusion:In this study, it was found that among different ecotypes of North Khorasan, methanolic extract of Verbascum thapsus of Bojnourd region had the most antibacterial effect on bacteria (Bacillus cereus and Staphylococcus aureus) however the extract was not effective on Escherichia coli.
Aim and Background:Wild plants in nature are valuable samples for use in various studies due to their characteristics such as resistance to adverse environmental conditions, resistance to pests and diseases and even having high active ingredients. Today, with the increasing resistance of bacteria to antibiotics, the use of medicinal plants has been welcomed.The present study aimed to investigate the effect of antibacterial effects of different ecotypes of Verbascum thapsus extract.
Materials and Methods:Verbascum thapsus from three regions of North Khorasan (Ashkhaneh, Bojnourd and Shirvan), after collection and drying, was extracted by methanol solvent by soaking and its antibacterial properties was investigation against( Escherichia coli, Bacillus cereus and Staphylococcus aureus) using disk and well diffusion , MIC (Minimum Inhibitory Concentration ) and MBC ( Minimum Bactericidal Concenteration) methods.
Results:The results showed that between different ecotypes of North Khorasan, Verbascom extract of Bojnourd region had the most inhition zone in disk and well diffusion method on Staphylococcus aureus bacteria were equal to 12.33 and 14.66 mm, respectively, and for Bacillus cereus bacteria were 11.33 and 13.66 mm, respectively And MIC and MBC of Verbascum extract of Bojnourd region on bacteria (Staphylococcus aureus) 12. mg/ml were observed as the most sensitive bacteria.
conclusion:In this study, it was found that among different ecotypes of North Khorasan, methanolic extract of Verbascum thapsus of Bojnourd region had the most antibacterial effect on bacteria (Bacillus cereus and Staphylococcus aureus) however the extract was not effective on Escherichia coli.
Keywords: Verbascum, Antibacterial, Escherichia coli, Bacillus cereus and Staphylococcus aureus, Iau Science.
Type of Study: Research Article |
Subject:
Cellular and molecular
Received: 2021/07/8 | Accepted: 2021/10/10 | Published: 2021/11/6
Received: 2021/07/8 | Accepted: 2021/10/10 | Published: 2021/11/6
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
