Volume 12, Issue 46 (3-2022)
NCMBJ 2022, 12(46): 75-88 |
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shamsi F. Investigation of human cellular response on cyclic RGD peptide immobilized on silicon surfaces via click chemistry. NCMBJ 2022; 12 (46) :75-88
URL: http://ncmbjpiau.ir/article-1-1458-en.html
URL: http://ncmbjpiau.ir/article-1-1458-en.html
School of Chemical and Biomolecular Engineering, University of Sydney, NSW 2006, Australia , fahimeh.shamsi@ymail.com
Abstract: (1707 Views)
Aim and Background: This study investigates the biological properties of cyclic RGD peptides being covalently immobilized via click chemistry onto alkyne terminated silicon surfaces.
Material and methods: At first cyclic RGD peptides were reacted with 4-azidophenyl isothiocyanate via a specific reaction. The azidated peptide was then covalently immobilized on an alkyne-terminated monolayer on silicon surfaces via the Cu (I)-catalyzed 1, 3-dipolarcycloaddition reaction. The surface structures of the attached peptide and control surfaces were characterized using X-ray photoelectron spectroscopy (XPS) and Fourier Transform Infrared (ATR-FTIR) Spectroscopy. Then after seeding human fibroblast cells on the peptide and control substrates, Scanning electron microscopy (SEM) and Laser scanning confocal microscopy (LSCM) was used to examine the morphology of human fibroblast cells. Adhesion and proliferation of human fibroblast cells on control and modified surfaces were analyzed by cell adhesion and (MTS) assay.
Results: XPS and ATR-FTIR analysis showed the cyclic RGD peptides were successfully immobilized on silicon surfaces. Scanning electron microscopy (SEM) and Laser scanning confocal microscopy (LSCM) demonstrated that human fibroblast cells homogenously distributed across the peptide-modified substrates and had a uniform appearance. Peptide-modified surfaces exhibited substantially superior cellular responses compared to those on control surfaces.
Conclusion: The research may suggest a procedure for the fabrication of biologically active surfaces in biosensor platforms.
Material and methods: At first cyclic RGD peptides were reacted with 4-azidophenyl isothiocyanate via a specific reaction. The azidated peptide was then covalently immobilized on an alkyne-terminated monolayer on silicon surfaces via the Cu (I)-catalyzed 1, 3-dipolarcycloaddition reaction. The surface structures of the attached peptide and control surfaces were characterized using X-ray photoelectron spectroscopy (XPS) and Fourier Transform Infrared (ATR-FTIR) Spectroscopy. Then after seeding human fibroblast cells on the peptide and control substrates, Scanning electron microscopy (SEM) and Laser scanning confocal microscopy (LSCM) was used to examine the morphology of human fibroblast cells. Adhesion and proliferation of human fibroblast cells on control and modified surfaces were analyzed by cell adhesion and (MTS) assay.
Results: XPS and ATR-FTIR analysis showed the cyclic RGD peptides were successfully immobilized on silicon surfaces. Scanning electron microscopy (SEM) and Laser scanning confocal microscopy (LSCM) demonstrated that human fibroblast cells homogenously distributed across the peptide-modified substrates and had a uniform appearance. Peptide-modified surfaces exhibited substantially superior cellular responses compared to those on control surfaces.
Conclusion: The research may suggest a procedure for the fabrication of biologically active surfaces in biosensor platforms.
Type of Study: Research Article |
Subject:
Cellular and molecular
Received: 2021/10/8 | Accepted: 2021/12/18 | Published: 2022/04/11
Received: 2021/10/8 | Accepted: 2021/12/18 | Published: 2022/04/11
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