Mohammadi E, momeni Z, jebelameli L. In vitro evaluation of the inhibitory effect of prodigiosin pigment from Serratia marsecsenes on Trichomonas vaginalis. NCMBJ 2023; 13 (51) :19-30
URL:
http://ncmbjpiau.ir/article-1-1509-en.html
Assistant Professor, Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran , zohreh.momeni@kiau.ac.ir
Abstract: (639 Views)
Aim and Background: Microbial pigments are a type of secondary metabolite that can play a good role due to their antimicrobial properties. Prodigiosin is an antimicrobial tri-pyrrole pigment that can be extracted from Serratia marcescens. Trichomonas vaginalis is a protozoan that enters the human reproductive system. Trichomoniasis is the most common non-viral sexually transmitted disease treated with metronidazole. Side effects and drug resistance are the two main problems of this drug that make doctors look for new treatments. The aim of this study was to extract prodigiosin pigment from Serratia marcesens to evaluate the antiparasitic properties of this pigment against Trichomonas vaginalis in vitro.
Materials and Methods: Serratia marcescens strain RTCC 2281 was purchased from Razi Institute and mass cultivated. prodigiosin was extracted using Ethyl acetate and acetone tools and the relativity of the pigment was confirmed by TLC chromatography, UV-Vis spectrophotometry, FTIR, and its effect on Trichomonas vaginalis and PC12 cell line was examined in different concentrations.
Results: Prodigiosin pigment at 10000 μg/ml in 24 hours and 5000 μg/ml and 2500 in 48 hours caused 100%, 92.89%, and 84.26% growth inhibition of the parasite. Its IC50 in 48 hours was 112.8 μg/ml and the CC50 of this pigment on the PC12 cell line reached 842.4 μg/ml.
Conclusion: This pigment was tested on Trichomonas vaginalis and its toxicity on the cell line was 7.46 times (SI = 7.46), so it can be concluded that prodigiosin pigment had an antiparasitic effect more comprehensive study of prodigiosin composition in vitro and in vivo is suggested.
Type of Study:
Research Article |
Subject:
Microbiology Received: 2022/07/3 | Accepted: 2022/09/18 | Published: 2023/06/22