Volume 15, Issue 57 (12-2024)
NCMBJ 2024, 15(57): 21-31 |
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Hajinourmohammadi A, Zargan J, Jafary H, Ebrahimi F. Investigating the antibacterial effects of extract and protein fractions isolated from Adenium obesum plant in laboratory conditions. NCMBJ 2024; 15 (57) :21-31
URL: http://ncmbjpiau.ir/article-1-1715-en.html
URL: http://ncmbjpiau.ir/article-1-1715-en.html
Department of Basic Science, Imam Hossein, University, Tehran, Iran. , jazrgan@ihu.ac.ir
Abstract: (166 Views)
Aim and Background: Infectious diseases caused by bacterial agents are one of the most common causes of death worldwide. Many of these agents are resistant to one or more antibiotics, some are multidrug resistant, and others are resistant to almost all commonly used antibiotics. Antimicrobial, anticancer, and antioxidant compounds have been reported from the extracts of different buffers derived from different plant species. In this study, the antibacterial activity of the buffered extract and protein fractions of the buffered extract of Adenium obesum were investigated.
Material and Methods: In this research, firstly, the electrophoretic-chromatographic pattern of the buffered plant leaf extract and then the antibacterial properties of the buffered extract in denatured and non-denatured forms of proteins as well as its protein fractions in the required concentrations in terms of micrograms per milliliter in Laboratory conditions (in-vitro) were tested using the minimum inhibitory concentration (MIC) assay for Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli and ANOVA statistical analysis.
Results: Different protein bands were observed in the SDS-PAGE spectrum of crude buffer extract. In the chromatographic study, 12 main peaks were separated and collected, of which 9 fractions contained protein. The results of this study showed that crude buffer extract with undenatured proteins from 0.02 μg/ml and crude buffer extract with denatured proteins from 0.04 μg/ml had a significant inhibitory effect on Staphylococcus aureus and Escherichia coli. The results of this study on the protein fractions of crude buffer extract also showed different effects on the tested bacteria, including the highest inhibition rate of Gram-positive bacteria related to fraction 8 with an average inhibition of 25% at a protein concentration of 0.01 μg/mL and similarly, it was shown in gram-negative bacteria related to fraction 3 with 0.16 μg/mL gelate and fraction 10 with 0.01 μg/mL gelate with an average inhibition of 11 percent.
Conclusion: The results of this study showed for the first time that denatured and non-denatured buffer extract and its protein fractions 3, 8, and 10 have antibacterial properties.
Material and Methods: In this research, firstly, the electrophoretic-chromatographic pattern of the buffered plant leaf extract and then the antibacterial properties of the buffered extract in denatured and non-denatured forms of proteins as well as its protein fractions in the required concentrations in terms of micrograms per milliliter in Laboratory conditions (in-vitro) were tested using the minimum inhibitory concentration (MIC) assay for Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli and ANOVA statistical analysis.
Results: Different protein bands were observed in the SDS-PAGE spectrum of crude buffer extract. In the chromatographic study, 12 main peaks were separated and collected, of which 9 fractions contained protein. The results of this study showed that crude buffer extract with undenatured proteins from 0.02 μg/ml and crude buffer extract with denatured proteins from 0.04 μg/ml had a significant inhibitory effect on Staphylococcus aureus and Escherichia coli. The results of this study on the protein fractions of crude buffer extract also showed different effects on the tested bacteria, including the highest inhibition rate of Gram-positive bacteria related to fraction 8 with an average inhibition of 25% at a protein concentration of 0.01 μg/mL and similarly, it was shown in gram-negative bacteria related to fraction 3 with 0.16 μg/mL gelate and fraction 10 with 0.01 μg/mL gelate with an average inhibition of 11 percent.
Conclusion: The results of this study showed for the first time that denatured and non-denatured buffer extract and its protein fractions 3, 8, and 10 have antibacterial properties.
Type of Study: Research Article |
Subject:
Cellular and molecular
Received: 2025/02/18 | Accepted: 2024/12/21 | Published: 2024/12/21
Received: 2025/02/18 | Accepted: 2024/12/21 | Published: 2024/12/21
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