Volume 15, Issue 57 (12-2024)
NCMBJ 2024, 15(57): 71-78 |
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Faezi Ghasemi M, Deshmir A. Cytotoxic effects of titanium dioxide nanoparticles (TiO2) on colon cancer (Coca-2) cell line. NCMBJ 2024; 15 (57) :71-78
URL: http://ncmbjpiau.ir/article-1-1719-en.html
URL: http://ncmbjpiau.ir/article-1-1719-en.html
Department of Microbiology, Lahijan Branch, Islamic Azad University, Lahijan, Iran , faezi_m@yahoo.com
Abstract: (178 Views)
Aim and Background: The use of nano materials has been proposed as a candidate for cancer cells treatments. The aim of this study was to investigate the cytotoxic effects of titanium oxide nanoparticles (TiO2) on colon cancer cell line (Caco-2).
Material and Methods: For the cell culture, the vial containing Caco-2 cells was cultured in RPMI 1640 medium. In order to evaluate the cytotoxic effects, titanium dioxide nanoparticles, in concentrations of 1, 5, 10, 25, 50 and 100 micrograms per milliliter were used on Caco-2 cells at time intervals of 24, 48 and 72 hours. Toxicity was measured by MTT method.
Results: The results showed that the survival percentage of Caco-2 cells in the used concentrations of TiO2 were 96.14%, 90.41%, 69.44%, 59.44%, 37.80, and 31.51%, respectively. Based on the obtained results, it was determined that titanium oxide nanoparticles in concentrations of 1 and 5 micrograms/ml have no significant effect on Caco-2 cells. However, by increasing the concentration of titanium nanoparticles from 10 μg/mL to 100 μg/mL, significant cytotoxic effects were observed in Caco-2 cells. Increasing titanium nanoparticle concentration from 10 to 100 μg/mL showed significant cytotoxic effects in Caco-2 cells compared to control (untreated) cells. The IC50 value was obtained at the concentration (50 μg/mL).
Conclusion: Overall, the present study shows that TiO2 can have a toxic effect on Caco-2 cells and be effective in development of anticancer drugs.
Material and Methods: For the cell culture, the vial containing Caco-2 cells was cultured in RPMI 1640 medium. In order to evaluate the cytotoxic effects, titanium dioxide nanoparticles, in concentrations of 1, 5, 10, 25, 50 and 100 micrograms per milliliter were used on Caco-2 cells at time intervals of 24, 48 and 72 hours. Toxicity was measured by MTT method.
Results: The results showed that the survival percentage of Caco-2 cells in the used concentrations of TiO2 were 96.14%, 90.41%, 69.44%, 59.44%, 37.80, and 31.51%, respectively. Based on the obtained results, it was determined that titanium oxide nanoparticles in concentrations of 1 and 5 micrograms/ml have no significant effect on Caco-2 cells. However, by increasing the concentration of titanium nanoparticles from 10 μg/mL to 100 μg/mL, significant cytotoxic effects were observed in Caco-2 cells. Increasing titanium nanoparticle concentration from 10 to 100 μg/mL showed significant cytotoxic effects in Caco-2 cells compared to control (untreated) cells. The IC50 value was obtained at the concentration (50 μg/mL).
Conclusion: Overall, the present study shows that TiO2 can have a toxic effect on Caco-2 cells and be effective in development of anticancer drugs.
Keywords: Titanium oxide Nanoparticles, Caco-2 cell line, Cytotoxic effects, Survival percentage.
Type of Study: Research Article |
Subject:
Cellular and molecular
Received: 2025/02/26 | Accepted: 2024/12/21 | Published: 2024/12/21
Received: 2025/02/26 | Accepted: 2024/12/21 | Published: 2024/12/21
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