Volume 15, Issue 57 (12-2024)
NCMBJ 2024, 15(57): 79-91 |
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Majdizadeh M, Eftekhari-Vash L, Zohrab-Beigi Y, Hghiralsadat B F, Khadem S M S. Characterization of PEGylated nano-niosomes containing Gemcitabine and evaluation of their cytotoxicity on human fibroblast (HFF) cell Line and MCF-7 breast cancer cell Line. NCMBJ 2024; 15 (57) :79-91
URL: http://ncmbjpiau.ir/article-1-1721-en.html
URL: http://ncmbjpiau.ir/article-1-1721-en.html
Mohammad Majdizadeh 
, Lida Eftekhari-Vash , Yasaman Zohrab-Beigi , Bibi Fatemeh Hghiralsadat , Seyyed Mohammad Sadegh Khadem
, Lida Eftekhari-Vash , Yasaman Zohrab-Beigi , Bibi Fatemeh Hghiralsadat , Seyyed Mohammad Sadegh Khadem
4. Department of Advanced Medical Sciences and Technologies, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran , Fhaghirosadat@gmail.com
Abstract: (152 Views)
Aim and Background: Gemcitabine is one of the most famous chemotherapy drugs and its use is subject to limitations. In the present study, Gemcitabine with the aim of increasing its antitumor properties was injected into the PEGylated niosomal nano-carriers and the toxicity of non-drug niosomes was studied on the fibroblast cell line (HFF) and the toxicity of the drug-containing niosomes to the breast cancer cell line (MCF-7).
Material and Methods: Niosomal vesicles were prepared using span 60(77.5%), cholesterol (17.5%) and polyethylene glycol (5%) by thin-film method and Gemcitabineis were loaded into the niosomes. Drug loading percentage was measured in nano-carriers. Their physicochemical properties were assayed, And the drug release rate was examined at 37 degrees Celsius, pH 7.4 and 42 degrees Celsius, pH 5.4. At the end, the toxicity of non-drug niosomes was studied on the fibroblast cell line (HFF) and the toxicity of the drug-containing niosomes on the MCF-7 breast cancer cell line ().
Results: PEGylated niosomes containing Gemcitabineis showed a size of 126.6nm, 39.1 ± 3.28% drug encapsulation efficiency, -29.91±0.42 mV of zeta potential. The release of the drug from niosomes was slow and morphological examination of the nanoparticles showed that they have a smooth and spherical surface. Cellular studies showed that non-drug niosomes produce little toxicity to HFF cell line and its toxicity are not significant. Also, the toxicity of noisome Gemcitabine compared to non-noisome Gemcitabine on the MCF-7 cell was significantly higher (p-value<0.01).
Conclusion: The results of this study indicate that PEGylated niosomal nano-carriers containing Gemcitabineis having the proper physicochemical properties, can be an appropriate carrier for drug delivery to breast cancer cells.
Material and Methods: Niosomal vesicles were prepared using span 60(77.5%), cholesterol (17.5%) and polyethylene glycol (5%) by thin-film method and Gemcitabineis were loaded into the niosomes. Drug loading percentage was measured in nano-carriers. Their physicochemical properties were assayed, And the drug release rate was examined at 37 degrees Celsius, pH 7.4 and 42 degrees Celsius, pH 5.4. At the end, the toxicity of non-drug niosomes was studied on the fibroblast cell line (HFF) and the toxicity of the drug-containing niosomes on the MCF-7 breast cancer cell line ().
Results: PEGylated niosomes containing Gemcitabineis showed a size of 126.6nm, 39.1 ± 3.28% drug encapsulation efficiency, -29.91±0.42 mV of zeta potential. The release of the drug from niosomes was slow and morphological examination of the nanoparticles showed that they have a smooth and spherical surface. Cellular studies showed that non-drug niosomes produce little toxicity to HFF cell line and its toxicity are not significant. Also, the toxicity of noisome Gemcitabine compared to non-noisome Gemcitabine on the MCF-7 cell was significantly higher (p-value<0.01).
Conclusion: The results of this study indicate that PEGylated niosomal nano-carriers containing Gemcitabineis having the proper physicochemical properties, can be an appropriate carrier for drug delivery to breast cancer cells.
Keywords: Key words: Gemcitabine, Nano-Niosome, MCF-7 cells, HFF cells, Breast Cancer, Kinetics of drug release.
Type of Study: Research Article |
Subject:
Cellular and molecular
Received: 2025/03/2 | Accepted: 2024/12/21 | Published: 2024/12/21
Received: 2025/03/2 | Accepted: 2024/12/21 | Published: 2024/12/21
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