Volume 15, Issue 59 (7-2025)
NCMBJ 2025, 15(59): 49-61 |
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Talebi G, Saffarian P, Hakemi-Vala M, Sadeghi A, Yadegar A. Investigating the effect of extracellular vesicles isolated from clinical strains of Helicobacter pylori on induction of epithelial-mesenchymal transition in insulin-resistant HepG2 cell line. NCMBJ 2025; 15 (59) :49-61
URL: http://ncmbjpiau.ir/article-1-1768-en.html
URL: http://ncmbjpiau.ir/article-1-1768-en.html
Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran , babak_y1983@yahoo.com
Abstract: (134 Views)
Aim and Background: Helicobacter pylori is a Gram-negative and microaerophilic pathogen that can release extracellular vesicles (EVs) containing proteins and different virulence factors. In addition to gastroduodenal diseases, this bacterium also plays a role in extra-gastroduodenal diseases such as insulin resistance (IR). Epithelial-mesenchymal transition (EMT) is considered a key process in the development of cancer, and several factors contribute to the initiation of this process, but the exact role of insulin in the EMT process has not yet been elucidated. This study aimed to investigate the effect of H. pylori-derived EVs on the induction of EMT in the insulin-resistant HepG2 cell line.
Materials and Methods: Two clinical isolates of H. pylori were obtained from patients with gastritis and peptic ulcer. Ultracentrifugation was used to extract H. pylori-derived EVs. In order to investigate the effect of EVs on the induction of EMT in IR, the HepG2 cell line was used to establish the IR model. An RT-qPCR assay was performed to evaluate the expression level of key genes involved in the EMT process, such as E-cadherin, snail, vimentin.
Results: The results of RT-qPCR showed that H. pylori-derived EVs can increase the expression of snail and vimentin and decrease the expression of E-cadherin genes under IR conditions.
Conclusion: The results obtained from the present study showed that H. pylori-derived EVs are capable of inducing the EMT process in the HepG2 cell line in addition to inducing hepatic IR.
Materials and Methods: Two clinical isolates of H. pylori were obtained from patients with gastritis and peptic ulcer. Ultracentrifugation was used to extract H. pylori-derived EVs. In order to investigate the effect of EVs on the induction of EMT in IR, the HepG2 cell line was used to establish the IR model. An RT-qPCR assay was performed to evaluate the expression level of key genes involved in the EMT process, such as E-cadherin, snail, vimentin.
Results: The results of RT-qPCR showed that H. pylori-derived EVs can increase the expression of snail and vimentin and decrease the expression of E-cadherin genes under IR conditions.
Conclusion: The results obtained from the present study showed that H. pylori-derived EVs are capable of inducing the EMT process in the HepG2 cell line in addition to inducing hepatic IR.
Keywords: Helicobacter pylori, insulin resistance, epithelial-mesenchymal transition, HepG2 cells, E-cadherin
Type of Study: Research Article |
Subject:
Microbiology
Received: 2025/09/8 | Accepted: 2025/07/1 | Published: 2025/07/1
Received: 2025/09/8 | Accepted: 2025/07/1 | Published: 2025/07/1
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