Volume 15, Issue 60 (12-2025)
NCMBJ 2025, 15(60): 15-25 |
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Rahimipour N, Sazegar H, Sharifnia F. Investigation of the Effect of Supernatant from Probiotic Bacteria Lactobacillus Sakei on the SK-OV-3 Cell Line in Ovarian Cancer. NCMBJ 2025; 15 (60) :15-25
URL: http://ncmbjpiau.ir/article-1-1781-en.html
URL: http://ncmbjpiau.ir/article-1-1781-en.html
- Assistant Professor, Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
Abstract: (26 Views)
Aim and Background: Ovarian cancer is one of the most dangerous types of malignancies in the female reproductive system and accounts for the highest mortality rates. This study aimed to induce apoptosis in SK-OV-3 cancer cells using metabolites from Lactobacillus sakei bacteria.
Materials and Methods: Different concentrations of Lactobacillus sakei supernatant (0.5, 1, 2, 3, 4, and 5 mg/mL) were collected and lyophilized. The appropriate cytotoxic concentration of the supernatant was determined at 24, 48, and 72 hours after treatment of SK-OV-3 cells. Then, the induction of apoptosis and the change in the expression of apoptosis-related genes were investigated under the influence of the IC50 concentration of the supernatant.
Results: The MTT test results showed that the concentration of 5 mg/mL of the supernatant had the highest cytotoxic effect on SK-OV-3 cells at 72 hours, and the flow cytometry results confirmed the induction of apoptosis at this concentration. Also, a decrease in the expression of BCL-2 and Survivin genes (anti-apoptotic genes) and an increase in the expression of BAX and FAS genes (pro-apoptotic genes) under the influence of the supernatant at this concentration and time were confirmed (p<0.05).
Conclusion: The results of this study demonstrate that the supernatant of Lactobacillus sakei bacteria is capable of inducing apoptosis and altering the expression of genes related to programmed cell death in SK-OV-3 cancer cells.
Materials and Methods: Different concentrations of Lactobacillus sakei supernatant (0.5, 1, 2, 3, 4, and 5 mg/mL) were collected and lyophilized. The appropriate cytotoxic concentration of the supernatant was determined at 24, 48, and 72 hours after treatment of SK-OV-3 cells. Then, the induction of apoptosis and the change in the expression of apoptosis-related genes were investigated under the influence of the IC50 concentration of the supernatant.
Results: The MTT test results showed that the concentration of 5 mg/mL of the supernatant had the highest cytotoxic effect on SK-OV-3 cells at 72 hours, and the flow cytometry results confirmed the induction of apoptosis at this concentration. Also, a decrease in the expression of BCL-2 and Survivin genes (anti-apoptotic genes) and an increase in the expression of BAX and FAS genes (pro-apoptotic genes) under the influence of the supernatant at this concentration and time were confirmed (p<0.05).
Conclusion: The results of this study demonstrate that the supernatant of Lactobacillus sakei bacteria is capable of inducing apoptosis and altering the expression of genes related to programmed cell death in SK-OV-3 cancer cells.
Type of Study: Research Article |
Subject:
Cellular and molecular
Received: 2025/12/15 | Accepted: 2025/12/1 | Published: 2025/12/1
Received: 2025/12/15 | Accepted: 2025/12/1 | Published: 2025/12/1
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