Volume 15, Issue 60 (12-2025)
NCMBJ 2025, 15(60): 41-60 |
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Mohebbi A, Hojati V, Aflatoonian R, Majidi Zolbin M. Investigation of targeted metabolites and organic acids resulting from cellular metabolism in the culture medium of cells extracted from endometriosis tissue using the GC/MS technique: a metabolomics approach. NCMBJ 2025; 15 (60) :41-60
URL: http://ncmbjpiau.ir/article-1-1784-en.html
URL: http://ncmbjpiau.ir/article-1-1784-en.html
Associate Professor, Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
Abstract: (28 Views)
Aim and Background: Endometriosis, a chronic inflammatory gynecological disorder, features ectopic endometrial-like glands and stroma, impacting ~10% of reproductive-age women and causing substantial morbidity. Limited insights into its pathogenesis prompted this metabolomics-based investigation to delineate metabolic perturbations in ectopic tissues under controlled physiological conditions, minimizing confounders.
Materials and Methods: Ectopic endometrial tissues from endometriosis patients were procured laparoscopically, while eutopic endometrial tissues from controls were sampled via aspiration during the proliferative menstrual phase. Post-collagenase digestion, 200,000 cells/well were cultured to confluence; media were then static for 72 h. Secreted metabolites were extracted and profiled using Shimadzu's organic acid diagnostic panel on a GC/MS system.
Results: Twenty-four metabolites were quantified. Ectopic samples exhibited significant reductions in fumaric acid and 2-ketoglutarate (P<0.05), alongside elevations in 4-hydroxyphenyllactic acid, palmitic acid, and lactate (P<0.05). Pyruvate, citrate, and 2-hydroxyisovalerate were nominally higher, albeit non-significantly. Pathway enrichment revealed disruptions in arginine biosynthesis, pyruvate metabolism, glycolysis/gluconeogenesis, and fatty acid pathways (including unsaturated fatty acid synthesis, β-oxidation, elongation, and GPI-anchor biosynthesis).
Conclusion: Krebs cycle dysregulation, coupled with upregulated inflammatory/oxidative stress metabolites, likely promotes ectopic cell survival and immune evasion. These metabolomic signatures underscore potential biomarkers for early endometriosis diagnosis and novel therapeutic targets.
Materials and Methods: Ectopic endometrial tissues from endometriosis patients were procured laparoscopically, while eutopic endometrial tissues from controls were sampled via aspiration during the proliferative menstrual phase. Post-collagenase digestion, 200,000 cells/well were cultured to confluence; media were then static for 72 h. Secreted metabolites were extracted and profiled using Shimadzu's organic acid diagnostic panel on a GC/MS system.
Results: Twenty-four metabolites were quantified. Ectopic samples exhibited significant reductions in fumaric acid and 2-ketoglutarate (P<0.05), alongside elevations in 4-hydroxyphenyllactic acid, palmitic acid, and lactate (P<0.05). Pyruvate, citrate, and 2-hydroxyisovalerate were nominally higher, albeit non-significantly. Pathway enrichment revealed disruptions in arginine biosynthesis, pyruvate metabolism, glycolysis/gluconeogenesis, and fatty acid pathways (including unsaturated fatty acid synthesis, β-oxidation, elongation, and GPI-anchor biosynthesis).
Conclusion: Krebs cycle dysregulation, coupled with upregulated inflammatory/oxidative stress metabolites, likely promotes ectopic cell survival and immune evasion. These metabolomic signatures underscore potential biomarkers for early endometriosis diagnosis and novel therapeutic targets.
Type of Study: Research Article |
Subject:
Biochemistry
Received: 2025/12/17 | Accepted: 2025/12/1 | Published: 2025/12/1
Received: 2025/12/17 | Accepted: 2025/12/1 | Published: 2025/12/1
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