Volume 15, Issue 60 (12-2025)
NCMBJ 2025, 15(60): 61-78 |
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Khosravy M, Hosseini F, Razavi M R, Khavari Nejad R A. Evaluation of antibiotic sensitivity, biofilm formation ability, and IL-1ra gene polymorphism in Acinetobacter baumannii isolates from patients with hospital-acquired pneumonia. NCMBJ 2025; 15 (60) :61-78
URL: http://ncmbjpiau.ir/article-1-1785-en.html
URL: http://ncmbjpiau.ir/article-1-1785-en.html
Department of biology, North Tehran Branch, Islamic Azad University, Tehran, Iran , hosseinimicrobiology@gmail.com
Abstract: (12 Views)
Aims and Background: This study aimed to evaluate the antibiotic sensitivity, biofilm formation capacity, and the association of IL-1ra gene polymorphism with pneumonia in Acinetobacter baumannii isolates obtained from patients with hospital-acquired pneumonia.
Materials and Methods: Among 100 patients with pneumonia, 30 A. baumannii isolates were identified from sputum samples. Blood samples were collected from these 30 patients as well as from 30 healthy individuals to analyze IL-1ra gene polymorphism using polymerase chain reaction (PCR) and sequencing. Antibiotic resistance was evaluated using the disk diffusion method and minimum inhibitory concentration (MIC). The expression levels of the biofilm-associated genes pgaA, blaPER-1, and bap were assessed by real-time PCR.
Results: All 30 isolates were resistant to most tested antibiotics and remained susceptible to colistin. The expression of blaPER-1 was lower than that of bap and pgaA in A. baumannii isolates (p< 0.001). A significant difference in the IL-1ra genotypic distribution was observed between the patient and healthy groups (p = 0.049), while allelic distribution did not differ significantly (p = 0.181).
Conclusion: A. baumannii strains show high resistance against a wide range of antibiotics. The expression of biofilm formation genes in A. baumannii showed that these genes increase pathogenicity through biofilm formation and other effective factors. There was a significant relationship between IL1-ra genomic polymorphism and A. baumannii pneumonia.
Materials and Methods: Among 100 patients with pneumonia, 30 A. baumannii isolates were identified from sputum samples. Blood samples were collected from these 30 patients as well as from 30 healthy individuals to analyze IL-1ra gene polymorphism using polymerase chain reaction (PCR) and sequencing. Antibiotic resistance was evaluated using the disk diffusion method and minimum inhibitory concentration (MIC). The expression levels of the biofilm-associated genes pgaA, blaPER-1, and bap were assessed by real-time PCR.
Results: All 30 isolates were resistant to most tested antibiotics and remained susceptible to colistin. The expression of blaPER-1 was lower than that of bap and pgaA in A. baumannii isolates (p< 0.001). A significant difference in the IL-1ra genotypic distribution was observed between the patient and healthy groups (p = 0.049), while allelic distribution did not differ significantly (p = 0.181).
Conclusion: A. baumannii strains show high resistance against a wide range of antibiotics. The expression of biofilm formation genes in A. baumannii showed that these genes increase pathogenicity through biofilm formation and other effective factors. There was a significant relationship between IL1-ra genomic polymorphism and A. baumannii pneumonia.
Type of Study: Research Article |
Subject:
Microbiology
Received: 2025/12/20 | Accepted: 2025/12/1 | Published: 2025/12/1
Received: 2025/12/20 | Accepted: 2025/12/1 | Published: 2025/12/1
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