Volume 16, Issue 62 (5-2026)
NCMBJ 2026, 16(62): 49-62 |
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Hemmati Dezaki Z, Parivar K, Goodarzi V, Nourani M R. Fabrication and Evaluation of a Cobalt-Containing Three-Layer Nanofibrous Scaffold and Its Antibacterial Properties. NCMBJ 2026; 16 (62) :49-62
URL: http://ncmbjpiau.ir/article-1-1821-en.html
URL: http://ncmbjpiau.ir/article-1-1821-en.html
Tissue Engineering and Regenerative Medicine Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Abstract: (322 Views)
Extended Abstract
Background and Aim: Bioactive glasses have attracted considerable attention in tissue engineering due to their excellent biocompatibility, bioactivity, and antibacterial properties. The aim of this study was to fabricate and evaluate a three-layer nanofibrous scaffold containing cobalt-doped bioactive glass nanoparticles for potential wound-healing applications. The scaffold was composed of polyurethane (PU), polycaprolactone (PCL), and collagen and was fabricated using the electrospinning technique to mimic the structure of the natural extracellular matrix.
Materials and Methods:The physicochemical properties of the fabricated scaffolds were characterized using Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). FTIR analysis confirmed the successful incorporation of the scaffold components and the formation of appropriate chemical interactions among them. SEM images revealed a porous nanofibrous structure favorable for cell attachment and proliferation.The biological performance of the scaffolds was evaluated using L929 fibroblast cells. The results demonstrated that the incorporation of cobalt-doped bioactive glass significantly enhanced cell adhesion, spreading, and proliferation compared with the control groups. In addition, the presence of collagen improved cell–scaffold interactions and provided a suitable microenvironment for tissue regeneration.
Results:The antibacterial activity of the scaffolds was assessed against Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus. The cobalt-containing scaffolds exhibited greater antibacterial activity and larger inhibition zones than the control and non-doped scaffolds. This effect was particularly noticeable against Gram-negative bacteria.
Conclusion: Overall, the results indicate that incorporating cobalt-doped bioactive glass nanoparticles into a three-layer nanofibrous scaffold significantly improves both biological and antibacterial properties. Therefore, the developed scaffold may serve as a promising candidate for skin tissue engineering, wound healing, and the treatment of chronic and infected wounds.
Background and Aim: Bioactive glasses have attracted considerable attention in tissue engineering due to their excellent biocompatibility, bioactivity, and antibacterial properties. The aim of this study was to fabricate and evaluate a three-layer nanofibrous scaffold containing cobalt-doped bioactive glass nanoparticles for potential wound-healing applications. The scaffold was composed of polyurethane (PU), polycaprolactone (PCL), and collagen and was fabricated using the electrospinning technique to mimic the structure of the natural extracellular matrix.
Materials and Methods:The physicochemical properties of the fabricated scaffolds were characterized using Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). FTIR analysis confirmed the successful incorporation of the scaffold components and the formation of appropriate chemical interactions among them. SEM images revealed a porous nanofibrous structure favorable for cell attachment and proliferation.The biological performance of the scaffolds was evaluated using L929 fibroblast cells. The results demonstrated that the incorporation of cobalt-doped bioactive glass significantly enhanced cell adhesion, spreading, and proliferation compared with the control groups. In addition, the presence of collagen improved cell–scaffold interactions and provided a suitable microenvironment for tissue regeneration.
Results:The antibacterial activity of the scaffolds was assessed against Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus. The cobalt-containing scaffolds exhibited greater antibacterial activity and larger inhibition zones than the control and non-doped scaffolds. This effect was particularly noticeable against Gram-negative bacteria.
Conclusion: Overall, the results indicate that incorporating cobalt-doped bioactive glass nanoparticles into a three-layer nanofibrous scaffold significantly improves both biological and antibacterial properties. Therefore, the developed scaffold may serve as a promising candidate for skin tissue engineering, wound healing, and the treatment of chronic and infected wounds.
Type of Study: Research Article |
Subject:
Microbiology
Received: 2026/06/20 | Accepted: 2026/05/31 | Published: 2026/05/31
Received: 2026/06/20 | Accepted: 2026/05/31 | Published: 2026/05/31
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