Volume 3, Issue 10 (6-2013)                   NCMBJ 2013, 3(10): 79-84 | Back to browse issues page

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Rahmani A, Seighali N, Ebrahimzadeh H. A study on Peroxidase Activity Alterations in Corms of Saffron (Crucus Sativus L.) exposed to different H2O2 concentrations and pH measurement during Dormancy and Waking. NCMBJ. 2013; 3 (10) :79-84
URL: http://ncmbjpiau.ir/article-1-338-en.html
, rh.abbas@gmail.com
Abstract:   (15535 Views)

Aim and Background. Per o xi d ases a re o n e o f t he m o st i m p o rta n t enz y mes that p artici pa t es in many p h y s i o lo g i c al p rocesses in p l a n ts. Peroxidases are heme containing proteins that utilize H2O2 in the oxidation of various organic and inorganic substrates. In this research, the activity of peroxidase, was investigated in Crocus sativus L. co r m s extracts . The activity of peroxidase was studied with different c on c e ntr at i o n o f H 2 O 2 and pH measurement .

Materials and Methods. In this study t h e pl a nt cor m s were p r epa r e d in Ju l y as dor m ant stage and Novamber as waking stage . C o r m s were us e d as t h e enz y me e x trac t i on sou r ce and w as u s e d f o r f u rt h e r st ud y .

Results. T h e re s ul t s s h o w e d t h at t h e o pti m u m p H o f e n z y m e activity i n dor m an c y s ta g e i s on e un i t higher t h a n th e w a k i ng . T he r e sul t s sug g ested t h at in c r e a si n g c on c e ntr at i o n o f H 2 O 2 up to t h e 8 0 mM i n cr e as e s the enz y ma t ic acti v ity a nd higher c oncentra ti ons of H 2 O 2 de c r ea s e the pe r oxida s e act i vity .

Conclusion. According t o th e k i n e ti cs r e s ults , i t se e m s t h at at le a s t tw o is o f or m s o f t h e p e r o x i d a s es ex i s t i n s a f fr on co r m s a n d s o ea c h i s o f or m c ould t a k e p a r t i n so m e dif f ere n t p r oc e s s es i n g r o w t h and d e v e l o p m ent.

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Type of Study: Research Article | Subject: Physiology
Received: 2013/05/27

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