Aim and Background: “Asparaginase” is an enzyme that plays a key role in curing acute lymphoblastic leukemia (ALL). Due to its antineoplastic property, it is widely used in chemotherapy. The goal of this study was to explore and introduce extracellular L-asparaginase enzymes produced by Bacillus spp. which potentially possess more desired serological properties and less side effects comparing to those of common commercial types. Materials and Methods: The Bacillus spp. isolated from protein rich soils were cultured in modified M9 media. Asparaginase producing colonies were differed based on color change in media. Then the produced enzyme was purified and its activity was evaluated. Also its molecular weight was measured. Those Bacillus spp. which were capable of producing enzymes with desired activity, were identified using molecular method.

Results: New L-asparaginase producer Bacillus strains were identified and the general properties of extracted enzymes were studied.

Conclusion: As extracted L-asparaginase from different bacteria has shown different anti-cancer effects, exploring for microorganisms that produce this enzyme, is one of the main ways that would lead us to an enzyme with ideal medical aspects.