Volume 5, Issue 19 (7-2015)
NCMBJ 2015, 5(19): 39-46 |
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Rouholamin S, Zahedi B, Saei A, Nazarian Firouzabadi F. COMPARISON OF THREE GENOMIC RNA EXTRACTION METHODS FROM POMEGRANATE PEEL. NCMBJ 2015; 5 (19) :39-46
URL: http://ncmbjpiau.ir/article-1-648-en.html
URL: http://ncmbjpiau.ir/article-1-648-en.html
Department of Herball Products, Lorestan University, Lorestan- Iran. , Sepidehrouholamin@yahoo.com
Abstract: (12253 Views)
Aims and Background: RNA extraction with high quantity and quality is a fundamental requirement for molecular biology studies. Presence of carbohydrate, tannins, proteins and polyphenols compounds have created problem for extracted RNA from woody plant. Therefore, it is important to a suitable extraction method to produce a good quality RNA.
Materials and Methods: Methods for RNA extraction were compared to choose the best method of RNA extraction from pomegranate (Punica granatum L.) peel. These methods included 1-Zarei et al 2- IHTB 3-CTAB–LiCl modified method.
Results: Of the methods tested, Zarei et al method is the most appropriate method for plants with high phenolic compounds, especially pomegranate. Hence the need for the use of dangerous chemical material and extraction kits is not felt.
Conclusion: At the end of experiment with attention to results and comparison between methods, Zarei et al method was selected as the best method for RNA extraction from fruit peel with high quantity, quality and purity. Maximum amount of RNA with high quality that extracted from Zarei et al method was 642 ng per 100 mg peel tissue.
Materials and Methods: Methods for RNA extraction were compared to choose the best method of RNA extraction from pomegranate (Punica granatum L.) peel. These methods included 1-Zarei et al 2- IHTB 3-CTAB–LiCl modified method.
Results: Of the methods tested, Zarei et al method is the most appropriate method for plants with high phenolic compounds, especially pomegranate. Hence the need for the use of dangerous chemical material and extraction kits is not felt.
Conclusion: At the end of experiment with attention to results and comparison between methods, Zarei et al method was selected as the best method for RNA extraction from fruit peel with high quantity, quality and purity. Maximum amount of RNA with high quality that extracted from Zarei et al method was 642 ng per 100 mg peel tissue.
Keywords: Spectrophotometry, RNA Extraction, Gel electrophoresis, Punica granatum, polyphenols compounds
Type of Study: Research Article |
Subject:
Cellular and molecular
Received: 2015/07/15 | Accepted: 2015/07/15 | Published: 2015/07/15
Received: 2015/07/15 | Accepted: 2015/07/15 | Published: 2015/07/15
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