Volume 5, Issue 19 (7-2015)
NCMBJ 2015, 5(19): 69-76 |
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farooghi N, maleki M, Ghasemi Germi K, Abdolmaleki A. The study of toxicity and teratogenic effects of Nerium oleander leave’s extract on chicken embryo. NCMBJ 2015; 5 (19) :69-76
URL: http://ncmbjpiau.ir/article-1-652-en.html
URL: http://ncmbjpiau.ir/article-1-652-en.html
Young Researchers & Elite Club, Hamedan Branch, Islamic Azad University, Hamedan, Iran. , Abdolmalekiarash1364@gmail.com
Abstract: (11498 Views)
Aims and Background: Nerium oleander is a medicinal plant with various biological activities such as antibacterial, antioxidant and cytotoxic effects. In the present work, methanolic extract of plant was used for the evaluation of toxicity and teratogenic effects on chicken embryo as an animal model.
Materials and Methods: Methanolic extracts were prepared. Methanolic extract solutions in DMSO were injected in air sac of chicken eggs at concentrations of 5, 10, 20, 40, 60 and 80 μg/egg. Seventy two hours after incubation, eggs were placed at 37.7-38C° incubator. Results: On the day 19 after incubation, eggs were opened and shown that 78.4, 65, 56.7, 46.7, 31.66 and 21.66 percent of embryos were alive, Results showed that LD50 was at the concentration of 43.60μg/egg. Morphological and skeletal abnormalities in the treatment groups showed that club foot and beak deformity were occurred in morphology while caudal vertebrate deletions, unossification or uncalcification of caudal vertebrates were appeared in skeleton of the Chickens.
Conclusion: The study of methanolic extract showed that, with increasing concentration, mortality increased. The extract in low concentrations had negligible toxicity and teratogenic effects which can be used as therapeutic aims.
Materials and Methods: Methanolic extracts were prepared. Methanolic extract solutions in DMSO were injected in air sac of chicken eggs at concentrations of 5, 10, 20, 40, 60 and 80 μg/egg. Seventy two hours after incubation, eggs were placed at 37.7-38C° incubator. Results: On the day 19 after incubation, eggs were opened and shown that 78.4, 65, 56.7, 46.7, 31.66 and 21.66 percent of embryos were alive, Results showed that LD50 was at the concentration of 43.60μg/egg. Morphological and skeletal abnormalities in the treatment groups showed that club foot and beak deformity were occurred in morphology while caudal vertebrate deletions, unossification or uncalcification of caudal vertebrates were appeared in skeleton of the Chickens.
Conclusion: The study of methanolic extract showed that, with increasing concentration, mortality increased. The extract in low concentrations had negligible toxicity and teratogenic effects which can be used as therapeutic aims.
Type of Study: Research Article |
Subject:
Cellular and molecular
Received: 2015/07/16 | Accepted: 2015/07/16 | Published: 2015/07/16
Received: 2015/07/16 | Accepted: 2015/07/16 | Published: 2015/07/16
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